...Philipp M. Tschaikner; Dominik Regele; Ruth Röck; Willi Salvenmoser; Dirk Meyer; Michel Bouvier; Stephan Geley; Eduard Stefan; Pia Aanstad ABSTRACT Hedgehog (Hh) ligands act as morphogens to direct patterning and proliferation during embryonic development. Protein kinase A (PKA) is a central...

.... Additionally, lack of AKAP3 caused global changes of the sperm proteome and mislocalization of sperm proteins, including accumulation of RNA metabolism and translation factors and displacement of PKA subunits in mature sperm, which may underlie misregulated PKA activity and immotility in sperm. Interestingly...

...Nancy Nader; Raphael Courjaret; Maya Dib; Rashmi P. Kulkarni; Khaled Machaca Vertebrate oocytes arrest at prophase of meiosis I as a result of high levels of cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) activity. In Xenopus , progesterone is believed to release meiotic arrest...

... raft reorganization and glycosylphosphatidylinositol-anchored protein shedding, which are signatures of sperm maturation, but few did so in Lcn2 null mice. Furthermore, we found that LCN2 binds to membrane phosphatidylethanolamine to reinforce lipid raft reorganization via a PKA-dependent mechanism...

...Miquel Tuson; Mu He; Kathryn V. Anderson Protein kinase A (PKA) is an evolutionarily conserved negative regulator of the hedgehog (Hh) signal transduction pathway. PKA is known to be required for the proteolytic processing event that generates the repressor forms of the Ci and Gli transcription...

...Steven A. Marks; Daniel Kalderon Hedgehog (Hh) signaling activates full-length Ci/Gli family transcription factors and prevents Ci/Gli proteolytic processing to repressor forms. In the absence of Hh, Ci/Gli processing is initiated by direct Pka phosphorylation. Despite those fundamental...

... in zebrafish by the E(Spl) factors Her5 and Her11, but the molecular cascades cooperating with Her5/11, and those accounting for their reduced effect in the medial IZ, remain unknown. We demonstrate here that the kinases Gsk3β and cAMP-dependent protein kinase A (PKA) are novel determinants of IZ formation...

..., functions of these transcription factors in neural crest cells are enhanced by PKA signaling. * Author for correspondence (e-mail: wakasama@mail.tains.tohoku.ac.jp ) 24 1 2006 © 2006. 2006 Neural crest Sox9 Slug Snail2 PKA BMP EMT Quail Neural crest...

... by localizing gurken ( grk ) RNA. Here, we report that Sqd is also essential for anteroposterior (AP) axis formation. We identified sqd in a screen for modifiers of the Protein Kinase A ( PKA ) oogenesis polarity phenotype. The AP defects of sqd mutant oocytes resemble those of PKA mutants in several ways...

... of rickets , which encodes a G-protein coupled receptor in the glycoprotein hormone family that is a putative bursicon receptor. Furthermore, stimulation of components downstream of bursicon, such as a membrane permeant analog of cAMP, or ectopic expression of constitutively active forms of G proteins or PKA...

... is HCO 3 - coupled to enhancement of evoked Ca 2+ entry and acceleration of flagellar beat frequency? We used a pharmacological approach to evaluate the hypothesis that stimulation of sperm adenylyl cyclase, increased cAMP content, and activation of PKA underlie the actions of HCO 3 - . Fig. 6A...

.... This asymmetric pattern correlates with normal development of the left-right axis. We now show that the Wnt and PKA signaling pathways control left-right determination in the chick embryo through Nodal . A Wnt/β-catenin pathway controls Nodal expression in and around Hensen's node, without affecting the upstream...

... to GVBD in nemertean oocytes that had been pre-injected with a cAMP fluorosensor. Such a rise in cAMP seemed to involve G-protein-mediated signaling and protein kinase A (PKA) stimulation, based on the inhibition of 5-HT-induced GVBD by specific antagonists of these transduction steps. Although...

... exhibit maximal Ci activity. It has been previously shown that stabilization does not suffice for activation. Consistent with this finding, our results suggest that the mechanisms regulating nuclear import, stabilization and activation are distinct from each other. Finally, we show that cos2 and pka , two...

... by two opposing forces, the action of the NLS and that of at least two regions targeting Ci to the cytoplasm. Further our data show that loss of PKA or Costal-2 activity does not fully mimic Hh signaling, demonstrating that Ci proteolysis and Ci activation are two distinct events which are regulated...

... protein kinase (PKA) by 8-Br-cAMP was not able to fully bypass the morphological defects of srfA − mutant spores, although this treatment induced spiA mRNA expression. Our results suggest that DdSRF is required for full maturation of spores and participates in the regulation of the expression of the spore...

... Differentiation Inducing Factor-1 (DIF1) together with cyclic AMP, or (2) 8-Br-cAMP, which is believed to penetrate cell membrane and activate cAMPdependent protein kinase (PKA). Most of the mutants were markedly defective in forming stalk cells in response to DIF-1 plus cAMP, confirming a close relationship...