.... Bone Osteoblasts Notch signaling Fracture healing Regeneration Zebrafish Orthopaedic Research Society http://dx.doi.org/10.13039/100009283 National Institutes of Health http://dx.doi.org/10.13039/100000002 F32 DE026346 R01 AR066028 R01 DE030660 R01 DK074482 R35...

...Stephanie C. McMillan; Jing Zhang; Hue-Eileen Phan; Shirine Jeradi; Leona Probst; Matthias Hammerschmidt; Marie-Andrée Akimenko ABSTRACT During zebrafish fin regeneration, blastema cells lining the epidermis differentiate into osteoblasts and joint cells to reconstruct the segmented bony rays. We...

...Benjamin E. Armstrong; Astra Henner; Scott Stewart; Kryn Stankunas Zebrafish innately regenerate amputated fins by mechanisms that expand and precisely position injury-induced progenitor cells to re-form tissue of the original size and pattern. For example, cell signaling networks direct osteoblast...

...Tingsheng Yu; Martin Graf; Joerg Renn; Manfred Schartl; Daria Larionova; Ann Huysseune; Paul Eckhard Witten; Christoph Winkler ABSTRACT osterix ( osx ; sp7 ) encodes a zinc-finger transcription factor that controls osteoblast differentiation in mammals. Although identified in all vertebrate...

... of the blastema. Chemical interference with Notch signaling resulted in a complete block of regeneration. Notch signaling was not required for the earliest known cellular processes during blastema formation, i.e. dedifferentiation and migration of osteoblasts, but specifically interfered with proliferation...

...Weiguang Wang; Na Lian; Yun Ma; Lingzhen Li; Richard C. Gallant; Florent Elefteriou; Xiangli Yang Atf4 is a leucine zipper-containing transcription factor that activates osteocalcin ( Ocn ) in osteoblasts and indian hedgehog ( Ihh ) in chondrocytes. The relative contribution of Atf4 in chondrocytes...

... of double and triple transgenic larvae, we monitored in vivo formation and activation of osteoclasts, as well as their interaction with osteoblasts. Upon Rankl induction, GFP-positive osteoclasts are first observed in the intervertebral regions and then quickly migrate to the surface of mineralized neural...

...Hong Zhou; Wendy Mak; Robert Kalak; Janine Street; Colette Fong-Yee; Yu Zheng; Colin R. Dunstan; Markus J. Seibel Glucocorticoids are important regulators of bone cell differentiation and mesenchymal lineage commitment. Using a cell-specific approach of osteoblast-targeted transgenic disruption...

... as critical regulators of this process. Here, we show that the homeoprotein engrailed 1(EN1) is expressed during embryonic and perinatal craniofacial bone development, where it localizes to the skeletogenic mesenchyme, and,subsequently, to calvarial osteoblasts and osteoprogenitors. Mice lacking En1 exhibit...