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Keywords: Organoids
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Journal Articles
Journal: Development
Development dev.200561.
Published: 28 July 2022
... that have proven difficult to generate efficiently and reproducibly from mouse embryonic stem cells: definitive endoderm and neural organoids. We present new protocols for rapid generation of nearly pure definitive endoderm and forebrain-patterned neural organoids that model the development of prethalamic...
Journal Articles
In collection:
Human development
Journal: Development
Development (2022) 149 (8): dev199904.
Published: 3 May 2022
... clearance. These functions are executed deep within tissues, which are often poorly accessible and subject to environmental variability, especially in humans. Here, we discuss how multicellular 3D systems in a dish – organoids – are transitioning from a proof-of-principle approach to a timely, robust...
Journal Articles
Journal: Development
Development (2022) 149 (8): dev200361.
Published: 22 April 2022
... reprogrammed cells in a degranulation-dependent manner. Importantly, in vivo partial reprogramming is strongly reduced by adoptive transfer of NK cells, whereas it is significantly increased by their depletion. Notably, in the absence of NK cells, the pancreatic organoids derived from OSKM-expressing mice...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2022) 149 (7): dev197947.
Published: 31 March 2022
... organoid systems can now provide unprecedented insights into the physical principles of human development. Table 1. Force-sensing mechanisms * Authors for correspondence ( Phuong.Le@childrens.harvard.edu ; Karl.Koehler@childrens.harvard.edu ) Competing interests K.R.K...
Journal Articles
Journal: Development
Development (2022) 149 (1): dev199840.
Published: 12 January 2022
... and differentiation. Multiple distinct trophoblast states were identified, representing progenitor CTB, column CTB, SCT precursors and EVT. Lineage trajectory analysis identified a progenitor origin that was reproduced in human trophoblast stem cell organoids. Heightened expression of basal cell adhesion molecule...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (24): dev192914.
Published: 15 December 2021
... ). Integrated live imaging and molecular profiling of embryoid bodies reveals a synchronized progression of early differentiation . Sci. Rep. 6 , 31623 . 10.1038/srep31623 Brassard , J. A. and Lutolf , M. P. ( 2019 ). Engineering stem cell self-organization to build better organoids...
Journal Articles
Journal: Development
Development (2021) 148 (18): dev199611.
Published: 8 September 2021
...Nicola Gritti; Jia Le Lim; Kerim Anlaş; Mallica Pandya; Germaine Aalderink; Guillermo Martínez-Ara; Vikas Trivedi ABSTRACT Recent years have seen a dramatic increase in the application of organoids to developmental biology, biomedical and translational studies. Organoids are large structures...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (17): dev199814.
Published: 3 September 2021
... regulators that drive liver cell development and primary liver cancers. We also discuss emerging models derived from pluripotent stem cells, 3D organoids and bioengineering for improved studies of signalling pathways in liver cancer and regenerative medicine. * Author for correspondence ( hoodless...
Journal Articles
Journal: Development
Development (2021) 148 (8): dev194357.
Published: 20 April 2021
... of intestinal crypt cell proliferation. Despite increasing data, in-depth analysis on their specific action on intestinal stem cells is lacking. By using ex vivo 3D organoid cultures and molecular approaches, we observed early responses to T3 involving the T3-metabolizing enzyme Dio1 and the transporter Mct10...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2020) 147 (7): dev177972.
Published: 6 April 2020
...Michael A. Lensink; Karin R. Jongsma; Sarah N. Boers; Jacquelien J. Noordhoek; Jeffrey M. Beekman; Annelien L. Bredenoord ABSTRACT Organoids are three-dimensional multicellular structures grown in vitro from stem cells and which recapitulate some organ function. They are derivatives of living...
Journal Articles
Journal: Development
Development (2019) 146 (20): dev181917.
Published: 15 October 2019
... of factors affecting symmetry breaking and spatial fate choice during this developmental process could prove valuable for in vitro differentiation and organoid formation. HEK 293 cells were split to three wells (12-well plate, 50% confluence). After 12 h, the cells were transfected with constitutive pCMV...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (17): dev177188.
Published: 2 September 2019
... . Recent advances in the field of stem cell biology and organoid culture systems allow the expansion and differentiation of tissue-specific progenitors and pluripotent stem cells in vitro into functional hair cells and otic-like neurons. We discuss how inner ear organoids have been developed and how...
Journal Articles
Journal: Development
Development (2019) 146 (13): dev177220.
Published: 21 June 2019
... stem cells. Joo-Hyeon Lee (Cambridge Stem Cell Institute, UK) presented work on the dynamics of lung AT2 cells, using in vivo models as well as alveolar organoids ( Lee et al., 2017 ). Upon injury induced with bleomycin, AT2 cells are activated, modulate metabolic pathways and then replace lung...
Journal Articles
Journal: Development
Development (2019) 146 (8): dev166173.
Published: 16 April 2019
...Emily M. Holloway; Meghan M. Capeling; Jason R. Spence ABSTRACT Organoids are complex three-dimensional in vitro organ-like model systems. Human organoids, which are derived from human pluripotent stem cells or primary human donor tissue, have been used to address fundamental questions about human...
Journal Articles
Journal: Development
Development (2019) 146 (1): dev171686.
Published: 9 January 2019
.... Zack; Melissa C. Skala; David M. Gamm ABSTRACT Numerous protocols have been described for producing neural retina from human pluripotent stem cells (hPSCs), many of which are based on the culture of 3D organoids. Although nearly all such methods yield at least partial segments of retinal structure...
Includes: Supplementary data
Journal Articles
In collection:
Neural development
Journal: Development
Development (2018) 145 (22): dev172049.
Published: 19 November 2018
...Denis Jabaudon; Madeline Lancaster ABSTRACT The field of developmental neuroscience is benefitting from recent technological advances that allow access to organogenesis in vitro via organoid preparations. These methods have been applied to better understanding neural identity, and have opened up...
Journal Articles
Journal: Development
Development (2018) 145 (19): dev165795.
Published: 1 October 2018
... and nutrient homeostasis. Recently developed human intestinal organoid models include EECs, but their rarity makes it difficult to study their formation and function. Here, we used the EEC-inducing property of the transcription factor NEUROG3 in human pluripotent stem cell-derived human intestinal organoids...
Includes: Supplementary data
Journal Articles
In collection:
Human development
Journal: Development
Development (2018) 145 (17): dev163550.
Published: 16 August 2018
... interests. 16 7 2018 16 7 2018 © 2018. Published by The Company of Biologists Ltd 2018 Summary: ‘ Endoderm Development and Disease’, a Keystone symposium held in February 2018, emphasized the importance of using a variety of models, including organoids, for gaining a more complete...
Journal Articles
Journal: Development
Development (2018) 145 (11): dev163501.
Published: 8 June 2018
.... Here, we summarise key findings from this meeting, which brought together emerging leaders to discuss various topics, including pluripotency, organoids, endogenous regeneration, transcriptional regulation, clinical applications and emerging technologies. Much like the classical compositions...
Journal Articles
Journal: Development
Development (2017) 144 (21): 3894–3906.
Published: 1 November 2017
... of the embryo. Here, we use gastruloids, mESC-based organoids, as a model system with which to study this process. We find that gastruloids localise T/Bra expression to one end and undergo elongation similar to the posterior region of the embryo, suggesting that they develop an AP axis. This process relies...
Includes: Supplementary data