... address the mechanism by which the transcription factors Nkx6.1 (Nkx6-1) and Nkx6.2 (Nkx6-2) orchestrate development of the endocrine alpha- and beta-cell lineages. Specifically, we assayed for the rescue of insulin-producing beta-cells in Nkx6.1 mutant mice upon restoring Nkx6 activity in select...

... other islet cell types develop normally. However, the molecular events downstream of NKX6.1, as well as the molecular pathways that ensure residualβ-cell formation in the absence of NKX6.1 are largely unknown. Here, we show that the Nkx6.1 paralog, Nkx6.2 , is expressed during pancreas development...

.... Indirect immunofluorescence analyses were performed on cryosections as described previously ( Briscoe et al.,2000 ). The following primary antibodies were used: rabbit anti-Nkx6.1 ( Jensen et al.,1996 ); guinea pig anti-Nkx6.2 and mouse anti-Evx1( Vallstedt et al., 2001 );mouse anti-Islet1, mouse anti-2H3...