...Chunzhu Song; Shannon N. Leahy; Emma M. Rushton; Kendal Broadie ABSTRACT Both mRNA-binding Fragile X mental retardation protein (FMRP; Fmr1) and mRNA-binding Staufen regulate synaptic bouton formation and glutamate receptor (GluR) levels at the Drosophila neuromuscular junction (NMJ) glutamatergic...

.... M. , Bowen , D. C. , Valenzuela , D. M. , Simmons , M. V. , Poueymirou , W. T. , Thomas , S. , Kinetz , E. , Compton , D. L. , Rojas , E. , Park , J. S. et al. ( 1996 ). The receptor tyrosine kinase MuSK is required for neuromuscular junction formation...

... differentiation at developing neuromuscular junctions remains unknown. By using axon severing and microfluidic chamber assays, we first showed that treatment of a protein synthesis inhibitor, cycloheximide, inhibits agrin-induced presynaptic differentiation in cultured Xenopus spinal neurons. Newly synthesized...

... balancing synaptic GluR subtypes remain unclear. Herein, we show that the two subtypes of GluRs (A and B) expressed at Drosophila neuromuscular junction synapses mutually antagonize each other in terms of their relative synaptic levels and affect subsynaptic localization of each other, as shown by super...

... in each hemisegment. Premature activation of Side in muscle precursors abrogated dorsal migration of motor nerves, resulting in larvae with a complete loss of neuromuscular junctions on dorsal-most muscles. High-speed videography showed that these larvae failed to maintain substrate contact...

... moiety. At the glutamatergic neuromuscular junction (NMJ) synapse, we find that Notum secreted from the postsynaptic muscle acts to strongly modulate synapse growth, structural architecture, ultrastructural development and functional differentiation. In N otum null flies, we find upregulated...

... of Drosophila BAR-domain proteins and identified islet cell autoantigen 69 kDa (ICA69) as one of the key regulators of morphological differentiation of the larval neuromuscular junction (NMJ). We show that Drosophila ICA69 colocalizes with α-Spectrin at the NMJ. The conserved N-BAR domain of ICA69 deforms...

...Julien Messéant; Jérôme Ezan; Perrine Delers; Konstantin Glebov; Carmen Marchiol; Franck Lager; Gilles Renault; Fadel Tissir; Mireille Montcouquiol; Nathalie Sans; Claire Legay; Laure Strochlic Understanding the developmental steps that shape formation of the neuromuscular junction (NMJ) connecting...

.... Extracellular matrix metalloproteinases (Mmps) regulated by secreted tissue inhibitors of metalloproteinases (Timps), cleave secreted and membrane-associated targets to sculpt the extracellular environment and modulate intercellular signaling. Here, we test the roles of Mmp at the neuromuscular junction (NMJ...

... by suppressing the denervation-dependent expression of Myog and Gdf5. Muscle Nerve Regeneration Denervation Neuromuscular junction Myogenin Dach2 Hdac9 Gdf5 Brachypodia Motor nerve Communication between nerve and muscle takes place at the neuromuscular junction (NMJ) where presynaptic...

... that modulate synaptic transmission and plasticity. Here, we define novel roles for a recently described scaffold protein, Dsychronic (DYSC), at the Drosophila larval neuromuscular junction. DYSC is the Drosophila homolog of whirlin/DFNB31, a PDZ domain protein linked to Usher syndrome, the most common form...

...Anne-Marie Lüchtenborg; Gonzalo P. Solis; Diane Egger-Adam; Alexey Koval; Chen Lin; Maxime G. Blanchard; Stephan Kellenberger; Vladimir L. Katanaev Drosophila neuromuscular junctions (NMJs) represent a powerful model system with which to study glutamatergic synapse formation and remodeling. Several...

... expression in a tissue-specific manner. Null mutants of Katanin 60 are pupal lethal, demonstrating that it is essential for viability. Loss-of-function mutants of Katanin 60 showed excess satellite boutons, reduced neurotransmission efficacy, and more enlarged cisternae at neuromuscular junctions...

...Mikolaj Sulkowski; Young-Jun Kim; Mihaela Serpe Effective communication between pre- and postsynaptic compartments is required for proper synapse development and function. At the Drosophila neuromuscular junction (NMJ), a retrograde BMP signal functions to promote synapse growth, stability...

...-N-acetylglucosaminyl-transferase I) function, and Mgat1 nulls are the most compromised N-glycosylation condition that survive long enough to permit synaptogenesis studies. At the Drosophila neuromuscular junction (NMJ), Mgat1 mutants display selective loss of lectin-defined carbohydrates...

... until E15.5, but is then partially lost in the most distal parts of the muscle. Ultrastructural observations of neuromuscular junctions (NMJs) in the distal region of the diaphragm reveal a normal organization, but a lower density of nerve terminals capped by terminal Schwann cells in E18.5 mutant when...

...Haitao Wu; Yisheng Lu; Arnab Barik; Anish Joseph; Makoto Mark Taketo; Wen-Cheng Xiong; Lin Mei Neuromuscular junction (NMJ) formation requires proper interaction between motoneurons and muscle cells. β-Catenin is required in muscle cells for NMJ formation. To understand underlying mechanisms, we...

...@mail.med.upenn.edu ) Competing interests statement The authors declare no competing financial interests. 22 12 2011 © 2012. Zebrafish Muscle specific kinase MuSK Unplugged Wnt11 Dishevelled Rab11 Recycling endosome Neuromuscular junction Planar cell polarity...

...Rebecca E. James; Heather T. Broihier The BMP pathway is essential for scaling of the presynaptic motoneuron arbor to the postsynaptic muscle cell at the Drosophila neuromuscular junction (NMJ). Genetic analyses indicate that the muscle is the BMP-sending cell and the motoneuron is the BMP...

... specifically to acetylcholine receptors in the postsynaptic membrane. We found that the intrinsic synaptic vesicle protein SV2A was localized at a minority of synaptic sites ( Fig. 1A ). Similar results were obtained in several limb and trunk muscles, although the fraction of neuromuscular junctions (NMJs...