... ) and by kidney/body weight ratios ( Fig. 2I ; Fig. S2G ). To assess the effect of Ntn1 loss on nephron generation, we performed acid maceration of adult kidneys followed by glomerular counting and found significantly fewer glomeruli in Ntn1 SPKO kidneys ( Fig. S2H ). Glomerular counts correlated with kidney...

... for renal cell fate choice and ciliogenesis in zebrafish. esrrγa deficiency altered proximodistal nephron patterning, decreased the multiciliated cell populace and disrupted ciliogenesis in the nephron, Kupffer's vesicle and otic vesicle. These phenotypes were consistent with interruptions in prostaglandin...

...Kathryn Duvall; Lauren Crist; Alison J. Perl; Naomi Pode Shakked; Praneet Chaturvedi; Raphael Kopan ABSTRACT Notch signaling promotes maturation of nephron epithelia, but its proposed contribution to nephron segmentation into proximal and distal domains has been called into doubt. We leveraged...

...Brooke E. Chambers; Eleanor G. Clark; Allison E. Gatz; Rebecca A. Wingert ABSTRACT A functional vertebrate kidney relies on structural units called nephrons, which are epithelial tubules with a sequence of segments each expressing a distinct repertoire of solute transporters. The transcriptiona`l...

...Brooke E. Chambers; Gary F. Gerlach; Eleanor G. Clark; Karen H. Chen; Anna E. Levesque; Ignaty Leshchiner; Wolfram Goessling; Rebecca A. Wingert ABSTRACT Renal functional units known as nephrons undergo patterning events during development that create a segmental array of cellular compartments...

...Caramai N. Kamei; Thomas F. Gallegos; Yan Liu; Neil Hukriede; Iain A. Drummond ABSTRACT Zebrafish kidneys use resident kidney stem cells to replace damaged tubules with new nephrons: the filtration units of the kidney. What stimulates kidney progenitor cells to form new nephrons is not known. Here...

... the formation of micro-organoids, each containing six to ten nephrons that were surrounded by endothelial and stromal populations. Single cell transcriptional profiling confirmed the presence and transcriptional equivalence of all anticipated renal cell types consistent with a previous organoid culture method...

... and progenitor populations during organogenesis. The mammalian kidney maintains a population of self-renewing stem cells that differentiate to give rise to thousands of nephrons, which are the functional units that carry out filtration to maintain physiological homeostasis. The polycomb repressive complex 2...

...Christopher J. Rowan; Winny Li; Hovhannes Martirosyan; Steven Erwood; Di Hu; Yun-Kyo Kim; Sepideh Sheybani-Deloui; Jaap Mulder; Joshua Blake; Lin Chen; Norman D. Rosenblum ABSTRACT Normal kidney function depends on the proper development of the nephron: the functional unit of the kidney. Reciprocal...

... developing nephron tubules reveals that cell division is not oriented in the longitudinal (or planar-polarized) axis. However, cell division is oriented perpendicular to the apical-basal axis. Absence of afadin in vivo leads to misorientation of apical-basal cell division in nephron tubules. Together...

...Lori L. O'Brien; Qiuyu Guo; YoungJin Lee; Tracy Tran; Jean-Denis Benazet; Peter H. Whitney; Anton Valouev; Andrew P. McMahon Nephron endowment is determined by the self-renewal and induction of a nephron progenitor pool established at the onset of kidney development. In the mouse, the related...

... mesenchyme cells to regulate their differentiation into nephrons. Here, we characterize the role of a putative binding partner of Fat4, the protocadherin Dchs1. Mutation of Dchs1 in mice leads to increased numbers of cap mesenchyme cells, which are abnormally arranged around the ureteric bud tips...

...Yuwen Li; Jiao Liu; Wencheng Li; Aaron Brown; Melody Baddoo; Marilyn Li; Thomas Carroll; Leif Oxburgh; Yumei Feng; Zubaida Saifudeen Contrary to its classic role in restraining cell proliferation, we demonstrate here a divergent function of p53 in the maintenance of self-renewal of the nephron...

... ( Alk3 IMP null ). Alk3 -deficient mice exhibit simple renal hypoplasia characterized by decreases in both kidney size and nephron number but normal tissue architecture. These defects are preceded by a decreased contribution of Alk3 -deleted cells to the metanephric blastema and reduced expression...

... hypoplasia with deficient nephron formation. In mutant kidneys, aberrant cell death occurs within the metanephric mesenchyme (MM),particularly in the cortical nephrogenic zone, which provides the progenitors for recurring rounds of nephron formation. Prior to mutant morphological changes, Wnt4 and Lim1...

... for this protein in the formation of organs derived from the intermediate mesoderm. * Author for correspondence (e-mail: N.Hastie@hgu.mrc.ac.uk) 10 02 1998 20 01 1999 © 1999 by Company of Biologists 1999 Adrenal Epicardium Nephron Transgenics WT1 YAC Mouse Human Wilms...

... of nephron development and epithelial cell differentiation which is amenable to genetic analysis. The pronephros consists of two nephrons with fused glomeruli and paired pronephric tubules and ducts. Nephron formation occurs after the differentiation of the pronephric duct with both the glomeruli and tubules...