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Development (2002) 129 (18): 4185–4192.
Published: 15 September 2002
...Chris R. L. Thompson; Mark S. Bretscher NEM-sensitive factor (NSF) is an essential protein required during membrane transport. We replaced part of the endogenous D. discoideum NSF gene ( nsfA ) by a PCR-mutagenised library and isolated 11 mutants temperature-sensitive (ts) for growth. Two...