... stem cell Mouse blastocyst Chimaera Live imaging Pluripotent stem cells provide a valuable system to explore intrinsic and extrinsic requirements for self-renewal in vitro . Murine embryonic stem cells (ESCs) are derived from epiblasts of late blastocysts ( Boroviak et al., 2014 ; Brook...

... affects the maturation of PrE precursors. Our results suggest that the processes of PrE and epiblast segregation, and cell fate progression are interdependent, and place aPKC as a central player in the segregation of epiblast and PrE progenitors in the mouse blastocyst. ‡ Author for correspondence...

... Y. , Pawson T. , Rossant J. ( 2006 ). Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway . Dev. Cell 10 , 615 – 624 . Cheng A. M. , Saxton T. M. , Sakai R. , Kulkarni S. , Mbamalu G...

... of gene expression in single cells isolated from the mouse blastocyst revealed that expression profiles largely fall within two cohorts: one characterised by PrE-specific genes, the other by EPI-specific genes ( Kurimoto et al., 2006 ). The gene encoding platelet-derived growth factor receptor alpha...

... in developmental potential. These findings challenge earlier conclusions concerning cell lineage and determination in the primitive ectoderm that were based on development in vitro of IS-treated ICMs from giant blastocysts. INTRODUCTION By the onset of implantation the inner cell mass (ICM) of the mouse blastocyst...