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Keywords: Morpholino
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Journal Articles
Journal: Development
Development (2020) 147 (19): dev193029.
Published: 12 October 2020
... cardiovascular system. In contrast, in zebrafish, previous studies have reported that although embryos injected with tek morpholinos (MOs) exhibit severe vascular defects, tek mutants display no obvious vascular malformations. To further investigate the function of zebrafish Tek, we generated a panel of loss...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2020) 147 (18): dev189373.
Published: 23 September 2020
..., we determine the role of both the Activin/Nodal and BMP pathways as they function in Capitella axis patterning. Antisense morpholino oligonucleotides were targeted to Ct-Smad2/3 and Ct-Smad1/5/8 , transcription factors specific to the Activin/Nodal and BMP pathways, respectively. Following...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2009) 136 (21): 3585–3595.
Published: 1 November 2009
... and depleted endogenous wnt4 translation using a morpholino oligonucleotide (MO) ( Saulnier et al., 2002 ). Embryos injected with notch-ICD mRNA and wnt4 MO were cultured to stage 32, where they were analysed fo r nephrin expression. Fig. 2. wnt4 overexpression induces the same phenotypes...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2008) 135 (17): 2865–2871.
Published: 1 September 2008
.... A previous study in zebrafish had proposed that a small group (<10) of early retinal growth cones were pioneers based on their significant separation from the next growth cones to follow( Stuermer, 1988 ). It is clear from our ath5 morpholino injections that these few growth cones are not functionally...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2007) 134 (13): 2449–2458.
Published: 1 July 2007
...-signaling-depleted embryos,suggesting a signal from rhombomere 5-6 may also be required for otic fate maintenance. We show that rhombomere 5 wnt8b expression is absent in both RA-signaling-depleted embryos and in fgf3;tcf2 double mutants,and inactivation of wnt8b in fgf3 mutants by morpholino injection...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2006) 133 (4): 725–735.
Published: 15 February 2006
... optic commissure; black dots mark the position of the optic recess. * Corresponding author (e-mail: Karlstrom@bio.umass.edu ) 19 12 2005 © 2006. 2006 AC Chiasm Commissure Ephrin Gfap Glial bridge Morpholino Netrin POC Semaphorin Slit...
Journal Articles
Journal: Development
Development (2005) 132 (16): 3643–3656.
Published: 15 August 2005
... the expression of Slit guidance molecules that then help to regulate glial cell position and axon guidance across the midline of the forebrain. 3 6 2005 ©2005. 2005 Zebrafish Cyclopamine Morpholino Axon guidance POC Chiasm Gfap Glia hedgehog slit you-too We have...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (15): 3381–3392.
Published: 1 August 2005
...Bruno Reversade; Hiroki Kuroda; Hojoon Lee; Ashley Mays; Edward M. De Robertis To address the patterning function of the Bmp2, Bmp4 and Bmp7 growth factors, we designed antisense morpholino oligomers (MO) that block their activity in Xenopus laevis . Bmp4 knockdown was sufficient to rescue...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (14): 3267–3279.
Published: 15 July 2005
...-specific marker N-tubulin by whole-mount in situ hybridization (MO-C: 1% embryos with reduction of N-tubulin,n =16; Fig. 1F ). By contrast, injection of morpholinos specific for Gli1 (MO-1), Gli2 (MO-2) or Gli3 (MO-3) inhibited primary neuron differentiation [MO-1: 66.7%, n =12 ( Fig. 1C ); MO-2:50...
Journal Articles
Journal: Development
Development (2005) 132 (10): 2489–2499.
Published: 15 May 2005
...)causes sirenomelia in the mouse. Tsg is a Bmp- and chordin-binding protein that has multiple effects on Bmp metabolism in the extracellular space; Bmp7 is one of many Bmps and is shown here to bind to Tsg. In Xenopus ,co-injection of Tsg and Bmp7 morpholino oligonucleotides (MO) has a synergistic effect...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2004) 131 (22): 5659–5669.
Published: 15 November 2004
... of a morpholino that blocks the mRNA splicing reaction that gives DeltaD its terminal valine, on which the interaction depends. Embryos treated in this way appear normal with respect to some known functions of DeltaD as a Notch ligand, including the control of somite segmentation, neurogenesis, and hypochord...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2004) 131 (20): 5091–5102.
Published: 15 October 2004
...`jumpstart' of otic specification that is maintained by the Dlx3b-Pax2a pathway. * Author for correspondence (e-mail: monte@uoneuro.uoregon.edu ) 9 7 2004 © 2004. 2004 dlx3b fgf3 fgf8 foxi1 Inner ear Morpholino Otic placode pax8 pax2a sox9a sox9b Zebrafish...
Journal Articles
Journal: Development
Development (2004) 131 (15): 3637–3647.
Published: 1 August 2004
.... One of the genes specifically expressed in the lateral plate mesoderm, and later in its derivative, the visceral mesoderm, is the Fox gene FoxF1. Its function is critical for Xenopus gut development, and embryos injected with FoxF1 morpholino display abnormal gut development. In the absence of FoxF1...
Journal Articles
Journal: Development
Development (2004) 131 (10): 2431–2441.
Published: 15 May 2004
... stage. We therefore examined its functions by blocking its translation using morpholino oligos. In Mixer-depleted embryos, the expression of many signaling ligands and transcription factors was affected. In particular, we found that the expression of several genes, including several normally expressed...
Journal Articles
Journal: Development
Development (2004) 131 (8): 1729–1740.
Published: 15 April 2004
... primary domains, one each in the head and trunk. Although the position of the anterior domain is conserved, the posterior domain is located at least four segments anterior to that of Drosophila . Knockdown phenotypes generated with morpholino oligonucleotides, as well as embryonic and parental RNA...
Journal Articles
Journal: Development
Development (2004) 131 (3): 613–627.
Published: 1 February 2004
... (hpf), and restricted to the head from 48 hpf onwards. Morpholino-induced prep1.1 loss-of-function caused significant apoptosis in the CNS. Hindbrain segmentation and patterning was affected severely, as revealed by either loss or defective expression of several hindbrain markers ( foxb1.2/mariposa...
Journal Articles
Journal: Development
Development (2003) 130 (20): 4943–4953.
Published: 15 October 2003
...Ana Cristina Silva; Mario Filipe; Klaus-Michael Kuerner; Herbert Steinbeisser; José António Belo We analyzed the endogenous requirement for Cerberus in Xenopus head development. `Knockdown' of Cerberus function by antisense morpholino oligonucleotides did not impair head formation in the embryo...
Journal Articles
Journal: Development
Development (2003) 130 (10): 2213–2224.
Published: 15 May 2003
... for correspondence (e-mail: monte@uoneuro.uoregon.edu ) 19 2 2003 © 2003. 2003 dlx3b dlx4b Inner ear Morpholino Olfactory placode sox9a sox9b Zebrafish To understand how Fgf signals may specify cells to form the otic placode,we studied the functions of four transcriptions...
Journal Articles
Journal: Development
Development (2003) 130 (8): 1549–1564.
Published: 15 April 2003
... development, we have analyzed the requirements for gli1 and gli2 during zebrafish embryogenesis. We report that detour ( dtr ) mutations encode loss-of-function alleles of gli1 . In contrast to mouse Gli1 mutants, dtr mutants and embryos injected with gli1 antisense morpholino oligonucleotides display defects...
Journal Articles
Journal: Development
Development (2002) 129 (22): 5279–5287.
Published: 15 November 2002
... and 6). Depletion of fgf3 by injection of antisense morpholino (fgf3-MO) suppresses the ear patterning defects in val/val embryos: Excess and ectopic hair cells are eliminated, expression of anterior otic markers is reduced or ablated, and zp23 is expressed throughout the medial wall of the otic vesicle...