...Brett Horr; Ryan Kurtz; Ankit Pandey; Benjamin G. Hoffstrom; Elizabeth Schock; Carole LaBonne; Dominique Alfandari ABSTRACT Monoclonal antibodies are powerful and versatile tools that enable the study of proteins in diverse contexts. They are often utilized to assist with identification...

...Rhonda Simper-Ronan; Kate Brilliant; Donna Flanagan; Marie Carreiro; Helen Callanan; Edmond Sabo; Douglas C. Hixson We have used monoclonal antibodies against cell-surface developmental epitopes in combination with micromagnetic beads to isolate phenotypically defined subpopulations...

... monoclonal antibodies to identify the absorptive and secretory cells in the epithelium, and we have used these antibodies to examine the part that Delta-Notch signalling plays in producing the diversity of intestinal cell types. Several Notch receptors and ligands are expressed in the gut. In particular...

... sheath cell monoclonal antibody immunoglobulin superfamily GPI-linked axon outgrowth insect The generation of cellular diversity during neuronal development is an important prerequisite for establishing the complex cellular connections necessary for nervous system function. The stereotypy...

... of functional SLF and c-Kit in fetuses with mutant alleles of Sl and W loci pro-duces only minor effects on the myeloid and early ery-throid progenitor cells in the fetal liver, although the level of the late erythroid progenitor cells is significantly affected. We used an anti-c-Kit monoclonal antibody...

...Richard J. Mullen; Charles R. Buck; Alan M. Smith ABSTRACT A battery of monoclonal antibodies (mAbs) against brain cell nuclei has been generated by repeated immunizations. One of these, mAb A60, recognizes a vertebrate nervous systemand neuron-specific nuclear protein that we have named NeuN ( Neu...

... endocytic and biosynthetic activities suggest that macrophages contribute major undefined functions during growth, turnover and modelling of fetal tissues. Abbreviations: Ab, antibody; ag, antigen; mAb, monoclonal antibody; MΦ, macrophage. † Correspondence and reprint requests * Cicely Wald...

...Elaine C. Seaver; Rolf O. Karlstrom; Michael J. Bastiani ABSTRACT To identify molecules important for pathfinding by growing axons, monoclonal antibodies (mAb) have been generated against embryonic grasshopper tissue. One mAb, 2B2, shows labeling exclusively in the nervous system. It recognizes...

...Burkhard Schlosshauer; Dorothee Grauer; Dieter Dütting; Jens Vanselow ABSTRACT To generate monoclonal antibodies, immunogen fractions were purified from embryonic chick retinae by temperature-induced detergent-phase separation employing Triton X-114. Under reducing conditions, the monoclonal...

...Olivier Pourquié; Monique Coltey; Jean-Léon Thomas; Nicole M. Le Douarin We have identified a glycoprotein (BEN) of 95 –100 ×10 3 M r using a monoclonal antibody. This protein is transiently expressed at the cell surface of the peripherally projecting neurons, i.e. motoneurons of the spinal cord...

...Burkhard Schlosshauer; Dieter Dütting; Monika Wild ABSTRACT Using an immunosuppression technique, the monoclonal antibody 2A1 has been generated specific for a 140 ×10 3 M r cytoplasmic-membrane-associated protein as shown by subcellular fractionation and Western blot analysis. The antigen...

... viridescens . J. Morph . 94 , 21 – 70 . 10.1002/jmor.1050940103 Chiquet , M. and Fambrough , D. M. ( 1984a ). Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis . J. Cell Biol . 98 , 1926 – 1936 . 10.1083/jcb.98.6.1926...

...E. A. Jones; H. R. Woodland ABSTRACT A monoclonal antibody, 2G9, has been identified and characterised as a marker of neural differentiation in Xenopus . The epitope is present throughout the adult central nervous system and in peripheral nerves. Staining is first detected in embryos at stage 21...

...Hiroshi Ohinata; Shin Tochinai; Chiaki Katagiri ABSTRACT To analyze the ontogenic emergence of leukocytes during early development, a mouse monoclonal antibody (IgG 1 ), designated as XL-1, was produced against the peritoneal macrophages of adult Xenopus laevis . The XL-1 determinant was expressed...

... the oviduct. The collection of unlabelled secreted proteins enabled us to produce a monoclonal antibody, which was used in the second series of experiments on oviduct-embryo interactions. The results confirmed that SOP 92 binds to the zona pellucida and moreover showed that this protein crosses the zona...

...J. Paul Knox; Susan Day; Keith Roberts A monoclonal antibody (JIM4) has been derived that recognizes a series of glycoproteins associated with the plasma membrane of a suspension-cultured carrot cell line and also an arabinogalactan proteoglycan secreted by the cultured cells. Immunocytochemistry...

... matrix basement membrane laminin monoclonal antibody The extracellular matrix (ECM) is thought to be important in the regulation of polarization, adhesion, migration and differentiation of cells during embryogenesis. The role and the nature of ECM components are well documented in vertebrates...

...Hilary J. Gower; Stephen E. Moore; George Dickson; VICKI L. Elsom; Ramesh Nayak; Frank S. Walsh ABSTRACT Monoclonal antibody 24.1D5 reacts specifically with an epitope expressed on the cell surface of mononucleate myoblasts in primary cultures of human skeletal muscle cells, but not with either...

... , H. ( 1988 ). Monoclonal antibodies against larval nervous system of Xenopus laevis; their specificities and application to analysis of neural development . Neuroscience 25 , 291 – 305 . 10.1016/0306-4522(88)90027-9 Nieuwkoop , P. D. & Faber , J. ( 1967 ). Normal...

...David L. Adelson; Tom Humphreys ABSTRACT We have generated and characterized a monoclonal antibody (McA Tg-HYL) that recognizes sea urchin hyalin as evidenced by immunofluorescence staining of the hyaline layer (HL) and immunoblot staining of the hyalin protein band. On immunoblots of HL extracts...