... to demonstrate that EIF4A3 promotes cortical development by controlling progenitor mitosis, cell fate and survival. Eif4a3 haploinsufficiency in mice causes extensive cell death and impairs neurogenesis. Using Eif4a3 ; p53 compound mice, we show that apoptosis has the most impact on early neurogenesis, while...

...-field microscopy; at later stages (32-512 cell), we relied on the localization of a fluorescent protein, Venus or Tomato, fused to a nuclear localization signal (NLS-3Venus or NLS-Tomato). The NLS protein accumulates in the nucleus during interphase, whereas from NEB to NER (mitosis) the signal...

... separation during cell division ( Hara and Fukagawa, 2020 ; Petry, 2016 ). The organization and temporal assembly of the spindle are all important features that dictate the outcome of division ( Lu and Johnston, 2013 ). One crucial aspect of cell division is the temporal control of mitosis...

... fusion, processes that are needed to form the first zygotic nucleus to start a new life. Drosophila meiosis Mitosis Spindle assembly Clathrin Bicaudal D BicD TACC CH-TOG Microtubule transport DeGradFP Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung...

... spindle attachments, abnormal centrosome number and relaxed cell cycle checkpoints ( Ganem et al., 2009 ; Soto et al., 2019 ). Regardless of the mechanism, chromosomes that are mis-segregated during mitosis will become encapsulated into micronuclei and can contribute to aneuploidy in subsequent divisions...

... of centrosomes and formation of the mitotic spindle ( Schmit and Ahmad, 2007 ). Previous studies have shown that Aurka, Cdk1 and other mitotic kinases are part of a feedback activation loop during mitosis entry ( Cazales et al., 2005 ; Van Horn et al., 2010 ). The activity of the Aurka-Ccnb1-Cdk1 axis peaks...

... activity. EMT MMP14 Delamination Mitosis Neural crest Polarity Matrix metalloproteinases (MMPs) are widely expressed in cancer and inflammation ( Bonnans et al., 2014 ; Khokha et al., 2013 ). Most of the attention has been focused on their role in matrix remodeling. However, we now know...

... in prolonged mitosis due to extension of early mitotic stages. This disruption is correlated with depletion of the populations of upper layer neurons in the cortex. In contrast, early cortical neural stem cells proliferate and expand normally in the absence of Sp2. These results indicate a stage-specific...

...-p26 silence nanos translation, downregulate a subset of microRNAs involved in germ cell differentiation and suppress ribosomal biogenesis via dMyc to limit germ cell mitosis. We also found that the human ortholog of Wh (WDR4) interacts with TRIM32 in human cells. Our results show that Wh...

... in interneuron loss. Our results demonstrate that interneuron development is intimately dependent upon progenitor mitosis duration and uncover a crucial post-transcriptional regulator of interneuron fate relevant for neurodevelopmental pathologies. This article has an associated ‘ The people behind the papers...

...Tadashi Nakagawa; Teng Zhang; Ryo Kushi; Seiji Nakano; Takahiro Endo; Makiko Nakagawa; Noriko Yanagihara; David Zarkower; Keiko Nakayama The mitosis-meiosis transition is essential for spermatogenesis. Specific and timely downregulation of the transcription factor DMRT1, and consequent induction...

... , D. , Porcellini , A. , Avvedimento , E. V. and Gottesman , M. E. ( 1996 ). Requirement for cAMP-PKA pathway activation by M phase-promoting factor in the transition from mitosis to interphase . Science   271 , 1718 - 1723 . 10.1126/science.271.5256.1718 Han , S. J...

.../0896-6273(94)90022-1 Lu , B. , Rothenberg , M. , Jan , L. Y. and Jan , Y. N. ( 1998 ). Partner of Numb colocalizes with Numb during mitosis and directs Numb asymmetric localization in Drosophila neural and muscle progenitors . Cell   95 , 225 - 235 . 10.1016/S0092-8674(00...

... Bax or Trp53 (also known as p53 ) rescues the survival of neural progenitors and reduces the growth restriction imposed by Aspm deletion. Our data show that Aspm functions to regulate mitosis and to mitigate DNA damage during CGNP cell division, causes microcephaly through progenitor apoptosis when...

... ( johnstca@unm.edu ) Competing interests statement The authors declare no competing financial interests. © 2013. Published by The Company of Biologists Ltd 2013 Spindle Microtubules Oriented cell division Polarity Mitosis Centrosome All multicellular animals...

... Chromatin Mitosis Epigenetics Gene expression For more than a century it has been known that ‘heritable traits’, or gene expression patterns, are faithfully transmitted from mother to daughter cells as they divide. For much of this time, we have also known that multicellular organisms generally...

...Leonardo G. Guilgur; Pedro Prudêncio; Tânia Ferreira; Ana Rita Pimenta-Marques; Rui Gonçalo Martinho Epithelial cells mostly orient the spindle along the plane of the epithelium (planar orientation) for mitosis to produce two identical daughter cells. The correct orientation of the spindle relies...

...Gonzalo Fernández-Miranda; Marianna Trakala; Javier Martín; Beatriz Escobar; Alejandra González; Norbert B. Ghyselinck; Sagrario Ortega; Marta Cañamero; Ignacio Pérez de Castro; Marcos Malumbres Mitosis is controlled by multiple kinases that drive cell cycle progression and prevent chromosome mis...

... the donor nucleus and the recipient cell was not required at the time of transfer, as long as synchrony was reached by the first mitosis. Our findings demonstrate the remarkable flexibility of the reprogramming process and support the importance of nuclear transcriptional regulators in mediating...

... mitosis are responsible for spindle positioning, leading to precise orientation of the cleavage furrow during ACD in the cells that give rise to the germ lineage in ascidians. References Barr F. A. , Gruneberg U. ( 2007 ). Cytokinesis: placing and making the final cut . Cell 131...