...Catherine C. Baker; Lorenzo Gallicchio; Neuza R. Matias; Douglas F. Porter; Lucineh Parsanian; Emily Taing; Cheuk Tam; Margaret T. Fuller ABSTRACT During meiosis, germ cell and stage-specific components impose additional layers of regulation on the core cell cycle machinery to set up an extended G2...

.... At a molecular level, VCP activity supports spermatocyte gene expression by downregulating a repressive histone modification, mono-ubiquitylated H2A (H2Aub), during meiosis. Remarkably, experimentally blocking H2Aub in VCP- RNAi testes is sufficient to overcome the meiotic-arrest phenotype and to promote...

...Oleksandr Kirsanov; Taylor A. Johnson; Bryan A. Niedenberger; Taylor N. Malachowski; Benjamin J. Hale; Qing Chen; Brad Lackford; Jiajia Wang; Anukriti Singh; Karen Schindler; Brian P. Hermann; Guang Hu; Christopher B. Geyer ABSTRACT Retinoic acid (RA) is the proposed mammalian ‘meiosis inducing...

... by completing an essential lengthy differentiation program before initiating meiosis. The molecular and cellular changes directing these developmental processes remain largely undefined. This wide gap in knowledge is due to two unresolved technical challenges: (1) lack of robust and reliable in vitro models...

... regulated translation of key cell cycle regulators during oocyte maturation, which is essential for sexual reproductivity. The Zar protein family has been implicated in translational regulation during female meiosis in a variety of vertebrate species and so far two members, Zar1 and Zar2 (also known...

... development. RTCB regulated the splicing of transcripts related to DNA methylation and DNA damage repair. In addition, it regulated the resumption of oocyte meiosis by affecting CDK1 activation. Moreover, the loss of RTCB suppressed zygotic genome activation (ZGA) and decreased translation at the global level...

... observed during distinct stages of gamete development, which include specific activities with critical meiotic functions. SWI/SNF chromatin remodelers are recognized regulators of gene transcription and DNA repair, but their composition and functions in meiosis are poorly understood. We have generated...

...Rudra Prasanna Banerjee; Martin Srayko ABSTRACT In most sexually reproducing animals, sperm entry provides the signal to initiate the final stages of female meiosis. In Caenorhabditis elegans , this signal is required for completion of female anaphase I and entry into meiosis II (MII). memi - 1/2/3...

... of parthenogenetic hybrids are genetic copies of their mother; however, the cellular mechanism enabling the production of unreduced cells is largely unknown. Here, we show that oocytes go through meiosis in three widespread, or even strongly invasive, obligate parthenogenetic complexes of geckos, namely in diploid...

..., which is associated with microtubules for the maintenance of spindle poles in mitosis, is also involved in actin dynamics in murine oocyte meiosis, co-localizing with microtubules during mouse oocyte maturation. Depletion of KIFC1 caused the failure of polar body extrusion, and we found that meiotic...

... of Biologists Ltd 2022 Summary: A component of the dose compensation complex promotes germline differentiation by regulating levels of a ribosomal protein in female Drosophila . Differentiation Meiosis Msl3 RpS19 Set2 and Rbfox1 National Institutes of Health 2R01GM111770-06...

...Mansour Aboelenain; Karen Schindler ABSTRACT Mammalian oocytes are transcriptionally quiescent, and meiosis and early embryonic divisions rely on translation of stored maternal mRNAs. Activation of these mRNAs is mediated by polyadenylation. Cytoplasmic polyadenylation binding element 1 (CPEB1...

... show that loss of EWSR1 in late meiotic prophase I does not affect proper meiosis completion, but does result in defective spermatid elongation and chromocenter formation in the developing germ cells. As a result, male mice lacking EWSR1 after pachynema are sterile. We found that, in Ewsr1 CKO round...

... of meiosis, especially in the adult, is not well understood. Here, we reveal that NP95 (also known as UHRF1) and DNMT1 – two essential proteins for maintenance DNA methylation – are co-expressed in spermatogonia and are necessary for meiosis in male germ cells. We find that Np95 - or Dnmt1 -deficient...

...Chun-Wei Feng; Guillaume Burnet; Cassy M. Spiller; Fiona Ka Man Cheung; Kallayanee Chawengsaksophak; Peter Koopman; Josephine Bowles ABSTRACT In mice, the entry of germ cells into meiosis crucially depends on the expression of stimulated by retinoic acid gene 8 ( Stra8 ). Stra8 is expressed...

...Daniel J. Barbosa; Vanessa Teixeira; Joana Duro; Ana X. Carvalho; Reto Gassmann ABSTRACT The microtubule motor cytoplasmic dynein 1 (dynein) and its essential activator dynactin have conserved roles in spindle assembly and positioning during female meiosis and mitosis, but their contribution...

... regulatory protein 1 (ESRP1) and found that it induced female infertility in mice. Esrp1 deletion perturbed spindle organization, chromosome alignment and metaphase-to-anaphase transformation in oocytes. The first polar body extrusion was blocked during oocyte meiosis owing to abnormal activation of spindle...

... receptor Meiosis Auxin-inducible degron Transcription factors perform diverse roles in the development of specialized cell types. Many work synergistically in gene regulatory networks to execute complex programs of cell differentiation ( Drapek et al., 2017 ; Levine and Davidson, 2005 ; Medwig...

...Yu Jiang; Hui-Ying Zhang; Zhen Lin; Ye-Zhang Zhu; Chao Yu; Qian-Qian Sha; Ming-Han Tong; Li Shen; Heng-Yu Fan ABSTRACT The most significant feature of meiosis is the recombination process during prophase I. CXXC finger protein 1 (CXXC1) binds to CpG islands and mediates the deposition of H3K4me3...

... and daughter cells. GSC Stem-cyst Abscission Mitosis Meiosis BMP Bam WDR4 Stem cell self-renewal and differentiation must be balanced for proper tissue homeostasis ( Nakada et al., 2011 ; Simons and Clevers, 2011 ; Spradling et al., 2011 ). This balance is known to be coordinated...