...Jessica Hitchcock; Katherine Hughes; Sara Pensa; Bethan Lloyd-Lewis; Christine J. Watson ABSTRACT Post-lactational mammary gland regression encompasses extensive programmed cell death and removal of milk-producing epithelial cells, breakdown of extracellular matrix components and redifferentiation...

...Oscar Cazares; Sharmila Chatterjee; Pinky Lee; Catherine Strietzel; J. W. Bubolz; Gwyndolen Harburg; Jon Howard; Sol Katzman; Jeremy Sanford; Lindsay Hinck ABSTRACT In the mammary gland, how alveolar progenitor cells are recruited to fuel tissue growth with each estrus cycle and pregnancy remains...

...Johanna I. Englund; Alexandra Ritchie; Leander Blaas; Hanne Cojoc; Nalle Pentinmikko; Julia Döhla; Sharif Iqbal; Manuel Patarroyo; Pekka Katajisto ABSTRACT Epithelial attachment to the basement membrane (BM) is essential for mammary gland development, yet the exact roles of specific BM components...

...Christine J. Watson; Walid T. Khaled ABSTRACT The mammary gland is a unique tissue and the defining feature of the class Mammalia. It is a late-evolving epidermal appendage that has the primary function of providing nutrition for the young, although recent studies have highlighted additional...

... a crucial role for RAB6A/A′ in the lactogenic function of the mammary gland and suggest that the trafficking pathways controlled by RAB6A/A′ depend on cell-type specialization and tissue context. Overall, these data demonstrate that Rab6a/a ′ expression is upregulated during pregnancy in luminal cells...

... the postnatal development of mammary gland and influences breast carcinogenesis by binding to the estrogen receptor ERα. ERα acts as a transcription factor but also elicits rapid signaling through a fraction of ERα expressed at the membrane. Here, we have used the C451A-ERα mouse model mutated...

.... Mammary glands appeared functional at the onset of lactation in mutant mice; however, myoepithelial cell morphology was markedly altered, suggesting cellular compensation mechanisms involving cytoskeleton reorganization. Notably, lactation was not sustained in mutant females, and the glands underwent...

...Jakub Sumbal; Zuzana Koledova ABSTRACT Fibroblast growth factor (FGF) signaling is crucial for mammary gland development. Although multiple roles for FGF signaling in the epithelium have been described, the function of FGF signaling in mammary stroma has not been elucidated. In this study, we...

...-glutamine, 5 μg/ml bovine insulin (Sigma-Aldrich), and penicillin-streptomycin (Gibco Life Technologies), as described ( Deugnier et al., 2002 ). Cells were routinely checked for mycoplasma contamination by Hoechst staining. Thoracic and inguinal mammary glands from three to six pubertal (6-week-old...

...Emilia Peuhu; Reetta Virtakoivu; Anja Mai; Anni Wärri; Johanna Ivaska In the mammary gland, vimentin intermediate filaments are expressed in stromal cells and in basal epithelial cell populations, including gland-reconstituting mammary stem cells, with largely undefined functions. Here, we have...

...Gillian J. Wilson; Kay D. Hewit; Kenneth J. Pallas; Claire J. Cairney; Kit M. Lee; Christopher A. Hansell; Torsten Stein; Gerard J. Graham Macrophages are important regulators of branching morphogenesis during development and postnatally in the mammary gland. Regulation of macrophage dynamics...

... of Health/Eunice Kennedy Shriver National Institute of Child Health and Human Development [Grant 5P01 HD038129-15 Functional Development of the Mammary Gland (PI, S.M.A.) Richer (PI Project 3)]; National Institutes of Health [Building Interdisciplinary Research Careers in Women's Health Scholarship K12...

... of the membrane-anchored matrix metalloproteinases MMP14 (MT1-MMP) and MMP15 (MT2-MMP), which drive epithelial cell invasion by remodeling the extracellular matrix and triggering associated signaling cascades. However, the roles that these proteinases play during mammary gland development in vivo remain undefined...

... motile epithelial cells whose collective migration drives mammary ductal elongation. Collective cell migration MAPK signaling Branching morphogenesis Epithelial development Tubulogenesis Mammary gland Epithelial tubes are an essential building block of animal organs but the mechanisms...

...Jamie L. Inman; Claire Robertson; Joni D. Mott; Mina J. Bissell The development of the mammary gland is unique: the final stages of development occur postnatally at puberty under the influence of hormonal cues. Furthermore, during the life of the female, the mammary gland can undergo many rounds...

...Xiaohong Zhang; Denisse Martinez; Zuzana Koledova; Guijuan Qiao; Charles H. Streuli; Pengfei Lu FGF signaling is essential for mammary gland development, yet the mechanisms by which different members of the FGF family control stem cell function and epithelial morphogenesis in this tissue...

... in early lethality. To explore the role of this basally restricted transcription factor in the mammary gland, we generated a cre -inducible mouse model. MMTV- or K14- cre -mediated deletion of Id4 led to a delay in ductal morphogenesis, consistent with previous findings using a germ-line knockout mouse...

... stratification are accomplished through receptor tyrosine kinase signaling dependent vertical cell divisions within the luminal epithelial cell layer. The mammary gland is a powerful model system for studying transitions between simple and stratified epithelial organization, and for the corresponding changes...

... that plays an essential role in ovulation by regulating the expression of the epidermal growth factor-like family of growth factors. Although several studies indicate a role for RIP140 in breast cancer, its role in the development of the mammary gland is unclear. By using RIP140-null and RIP140 transgenic...

...Emanuela S. Milani; Heike Brinkhaus; Regula Dueggeli; Ina Klebba; Urs Mueller; Michael Stadler; Hubertus Kohler; Matthew J. Smalley; Mohamed Bentires-Alj Tyrosine phosphorylation plays a fundamental role in mammary gland development. However, the role of specific tyrosine phosphatases...