... ). The preparation of Drosophila embryos for live-imaging using the hanging drop protocol . J. Vis. Exp. , e1206 . 10.3791/1206 Roy , S. , Ernst , J. , Kharchenko , P. V. , Kheradpour , P. , Negre , N. , Eaton , M. L. , Landolin , J. M. , Bristow , C. A. , Ma , L...

...Emmanuel Martin; Magali Suzanne ABSTRACT Cell and developmental biology increasingly require live imaging of protein dynamics in cells, tissues or living organisms. Thanks to the discovery and development of a panel of fluorescent proteins over the last decades, live imaging has become a powerful...

..., but successful removal of the MES. Novel static- and live-imaging approaches reveal that the MES is removed through streaming migration of epithelial trails and islands to reach the oral and nasal epithelial surfaces. Epithelial trail cells that express the basal epithelial marker ΔNp63 begin to express periderm...

... in avian embryos during primitive streak development before stage (st.) 4, this migration at later stages of brain development has remained uninvestigated. By live imaging of epiblast cells sparsely labeled with green fluorescence protein, we investigated anterior epiblast cell migration to form individual...

..., this is the first quantitative live imaging of meristem development in a system with numerous whorls of floral organs, as well as an apocarpous gynoecium. This study provides crucial information for our understanding of how the spatial-temporal regulation of floral meristem behavior is achieved in both evolutionary...

...Azelle Hawdon; Asma Aberkane; Jennifer Zenker ABSTRACT With the advancement of cutting-edge live imaging technologies, microtubule remodelling has evolved as an integral regulator for the establishment of distinct differentiated cells. However, despite their fundamental role in cell structure...

... and to produce sibling cystoblasts that will in turn enter differentiation. GSCs and cystoblasts contain spectrosomes, membranous structures essential for orientation of the mitotic spindle and that, particularly in GSCs, change shape depending on the cell cycle phase. Using live imaging and a fusion protein...

... and applications to lineage tracing in live-imaging data and outline software methodologies that can be applied to various cell-tracking challenges. * These authors contributed equally to this work ‡ Author for correspondence ( kmcdole@mrc-lmb.cam.ac.uk ) Competing interests The authors...

... regulation of hb , including quantitative live-imaging studies of the output from the proximal enhancer and P2 promoter, with nascent transcription detected as early as nc9-10 ( Garcia et al., 2013 ; Lucas et al., 2013 ). In comparison, little is known about the translatability of zygotic hb mRNAs...

... positive feedback. Summary: Time-lapse three-dimensional imaging is a powerful method to analyze spatiotemporal gene expression changes of individual cells in inner and outer hair cell fate control of embryonic cochlear epithelium. Atoh1 Cochlea Hair cell Live imaging Mouse...

...-lethal activation of apoptotic signaling with the combination of a sensitive indicator for caspase 3 activation and in vivo live-imaging analysis of Drosophila . During thorax closure in pupal development, caspase 3 activation was specifically observed at the leading edge cells, with no signs...

...Huili Li; Jing Zhang; Lee Niswander ABSTRACT Micronutrition is essential for neural tube closure, and zinc deficiency is associated with human neural tube defects. Here, we modeled zinc deficiency in mouse embryos, and used live imaging and molecular studies to determine how zinc deficiency affects...

... and that this behavior depends upon mechanical forces. Quantitative live imaging and mathematical modeling allow us to correlate this tissue convergence with the underlying flow forces. We predict that tissue convergence is associated with the direction of the mean wall shear stress and of the gradient of harmonic phase...

.... Confocal microscopy Live imaging Quail embryo Axis elongation Proliferation PSM Multi-tissue Tissue deformations Morphogenesis Summary: A combination of time-lapse imaging and image analysis techniques allows visualization and quantification of the complex choreography and sliding behaviors...

... morphological assessments of cellular rearrangements and time-lapse imaging to visualize cochlear remodeling in mouse. Analysis of cell redistribution showed that the cochlea extends through a combination of radial intercalation and cell growth. Live imaging demonstrated that concomitant cellular intercalation...

... from GFP, Venus and Cerulean fluorophores reveal apoptotic cell dynamics throughout the whole apoptotic process within fixed and living Drosophila tissues. Apoptosis Caspase-activity sensor Drosophila Live imaging The main function of epithelia is to form and maintain a physical...

...) ( Fig. 1 ). Recent live-imaging studies of developing ISVs, cerebral blood vessels and the SIV of the zebrafish embryo revealed a series of cell behaviors that succeed each other in a stereotypical mode, starting from initial filopodial contacts and ending with a novel, multicellular connection...

... © 2016. Published by The Company of Biologists Ltd 2016 Summary: 3D analyses of cardiac valve formation in zebrafish illustrate the complex cellular rearrangements that occur during this process in vivo . Cardiac valves Endocardium Live imaging Notch Wnt Zebrafish Apc...

... and fate choice in the zebrafish retina - a continuously growing neural tissue - reveals key features of late retinal neurogenesis. Stem cells Progenitor cells Retina Ciliary marginal zone Live imaging Clonal analysis Zebrafish The retinas of fish and amphibians grow continuously...

... stem cell Mouse blastocyst Chimaera Live imaging Pluripotent stem cells provide a valuable system to explore intrinsic and extrinsic requirements for self-renewal in vitro . Murine embryonic stem cells (ESCs) are derived from epiblasts of late blastocysts ( Boroviak et al., 2014 ; Brook...