...Alexis Villars; Gaëlle Letort; Léo Valon; Romain Levayer ABSTRACT Accurately counting and localising cellular events from movies is an important bottleneck of high-content tissue/embryo live imaging. Here, we propose a new methodology based on deep learning that allows automatic detection...

.... The probabilistic nature of transcription has led to studies analyzing the effect of enhancer–promoter interactions and bursting activities. Live-imaging studies on enhancer–promoter dynamics suggest that, at least in the case of the pluripotency gene Sox2 and its distal enhancer SCR, there is little correlation...

... 20 embryos (for dsRNAi experiments) and 100 embryos (for transgenic injections) were aligned on a block of agar and transferred to a coverslip using a thin layer of heptane glue. If the injected embryos were for live imaging, embryos would be aligned with their ventral side facing the glue...

...Emmanuel Martin; Magali Suzanne ABSTRACT Cell and developmental biology increasingly require live imaging of protein dynamics in cells, tissues or living organisms. Thanks to the discovery and development of a panel of fluorescent proteins over the last decades, live imaging has become a powerful...

..., but successful removal of the MES. Novel static- and live-imaging approaches reveal that the MES is removed through streaming migration of epithelial trails and islands to reach the oral and nasal epithelial surfaces. Epithelial trail cells that express the basal epithelial marker ΔNp63 begin to express periderm...

... in avian embryos during primitive streak development before stage (st.) 4, this migration at later stages of brain development has remained uninvestigated. By live imaging of epiblast cells sparsely labeled with green fluorescence protein, we investigated anterior epiblast cell migration to form individual...

..., this is the first quantitative live imaging of meristem development in a system with numerous whorls of floral organs, as well as an apocarpous gynoecium. This study provides crucial information for our understanding of how the spatial-temporal regulation of floral meristem behavior is achieved in both evolutionary...

...Azelle Hawdon; Asma Aberkane; Jennifer Zenker ABSTRACT With the advancement of cutting-edge live imaging technologies, microtubule remodelling has evolved as an integral regulator for the establishment of distinct differentiated cells. However, despite their fundamental role in cell structure...

... and to produce sibling cystoblasts that will in turn enter differentiation. GSCs and cystoblasts contain spectrosomes, membranous structures essential for orientation of the mitotic spindle and that, particularly in GSCs, change shape depending on the cell cycle phase. Using live imaging and a fusion protein...

... and applications to lineage tracing in live-imaging data and outline software methodologies that can be applied to various cell-tracking challenges. Beyond the introduction and advancement of new microscopes capable of imaging large and sensitive specimens (of which there has been an explosion in recent years...

... translational repression of zygotic hunchback mRNAs. Hunchback Drosophila embryo Translation SunTag Live imaging MS2 The gap gene hunchback ( hb ) is essential for body plan specification during Drosophila embryogenesis, primarily by establishing the gene expression boundaries...

... of individual cells in inner and outer hair cell fate control of embryonic cochlear epithelium. Atoh1 Cochlea Hair cell Live imaging Mouse The sensory epithelium of the mammalian cochlea, called the organ of Corti, contains mechanosensory hair cells (HCs) and supporting cells (SCs...

...-lethal activation of apoptotic signaling with the combination of a sensitive indicator for caspase 3 activation and in vivo live-imaging analysis of Drosophila . During thorax closure in pupal development, caspase 3 activation was specifically observed at the leading edge cells, with no signs...

...Huili Li; Jing Zhang; Lee Niswander ABSTRACT Micronutrition is essential for neural tube closure, and zinc deficiency is associated with human neural tube defects. Here, we modeled zinc deficiency in mouse embryos, and used live imaging and molecular studies to determine how zinc deficiency affects...

... and that this behavior depends upon mechanical forces. Quantitative live imaging and mathematical modeling allow us to correlate this tissue convergence with the underlying flow forces. We predict that tissue convergence is associated with the direction of the mean wall shear stress and of the gradient of harmonic phase...

.... Natl. Acad. Sci. USA   106 , 20812 - 20817 . 10.1073/pnas.0906464106 Sugiyama , M. , Saitou , T. , Kurokawa , H. , Sakaue-Sawano , A. , Imamura , T. , Miyawaki , A. and Iimura , T. ( 2014 ). Live imaging-based model selection reveals periodic regulation...

... morphological assessments of cellular rearrangements and time-lapse imaging to visualize cochlear remodeling in mouse. Analysis of cell redistribution showed that the cochlea extends through a combination of radial intercalation and cell growth. Live imaging demonstrated that concomitant cellular intercalation...

... and adult male gonads . Nat. Protoc.   1 , 1725 - 1731 . 10.1038/nprot.2006.235 Bardet , P.-L. , Kolahgar , G. , Mynett , A. , Miguel-Aliaga , I. , Briscoe , J. , Meier , P. and Vincent , J.-P. ( 2008 ). A fluorescent reporter of caspase activity for live imaging...

... vessels, and hence is fundamental for vascular network formation. Anastomosis can occur between two sprouts and involve two tip cells (‘head-to-head’ anastomosis), or between sprouts and a functional blood vessel, involving only one tip cell (‘head-to-side’ anastomosis) ( Fig. 1 ). Recent live-imaging...

... analyses of cardiac valve formation in zebrafish illustrate the complex cellular rearrangements that occur during this process in vivo . Cardiac valves Endocardium Live imaging Notch Wnt Zebrafish Apc The correct formation and function of cardiac valves is crucial to ensure efficient...