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Keywords: Live imaging
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Journal Articles
Journal: Development
Development (2024) 151 (5): dev202132.
Published: 4 March 2024
...Shufan Lin; Bomyi Lim ABSTRACT Although fluctuations in transcription factor (TF) dosage are often well tolerated, TF dosage modulation can change the target gene expression dynamics and result in significant non-lethal developmental phenotypes. Using MS2/MCP-mediated quantitative live imaging...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2023) 150 (13): dev201747.
Published: 30 June 2023
...Alexis Villars; Gaëlle Letort; Léo Valon; Romain Levayer ABSTRACT Accurately counting and localising cellular events from movies is an important bottleneck of high-content tissue/embryo live imaging. Here, we propose a new methodology based on deep learning that allows automatic detection...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2023) 150 (12): dev201546.
Published: 20 June 2023
... presents compelling examples of the roles and regulation of transcriptional bursting in development and explores the question of how stochastic transcription leads to deterministic organism development. Development Enhancers Live imaging Modeling Promoters Transcriptional bursting...
Journal Articles
Journal: Development
Development (2022) 149 (21): dev200292.
Published: 31 October 2022
... 20 embryos (for dsRNAi experiments) and 100 embryos (for transgenic injections) were aligned on a block of agar and transferred to a coverslip using a thin layer of heptane glue. If the injected embryos were for live imaging, embryos would be aligned with their ventral side facing the glue...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2022) 149 (16): dev200495.
Published: 22 August 2022
...Emmanuel Martin; Magali Suzanne ABSTRACT Cell and developmental biology increasingly require live imaging of protein dynamics in cells, tissues or living organisms. Thanks to the discovery and development of a panel of fluorescent proteins over the last decades, live imaging has become a powerful...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2022) 149 (10): dev200181.
Published: 26 May 2022
..., but successful removal of the MES. Novel static- and live-imaging approaches reveal that the MES is removed through streaming migration of epithelial trails and islands to reach the oral and nasal epithelial surfaces. Epithelial trail cells that express the basal epithelial marker ΔNp63 begin to express periderm...
Includes: Supplementary data
Journal Articles
Journal Articles
In collection:
Plant development
Journal: Development
Development (2022) 149 (4): dev200256.
Published: 17 February 2022
..., this is the first quantitative live imaging of meristem development in a system with numerous whorls of floral organs, as well as an apocarpous gynoecium. This study provides crucial information for our understanding of how the spatial-temporal regulation of floral meristem behavior is achieved in both evolutionary...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (20): dev199909.
Published: 28 October 2021
...Azelle Hawdon; Asma Aberkane; Jennifer Zenker ABSTRACT With the advancement of cutting-edge live imaging technologies, microtubule remodelling has evolved as an integral regulator for the establishment of distinct differentiated cells. However, despite their fundamental role in cell structure...
Journal Articles
Journal Articles
Journal: Development
Development (2021) 148 (18): dev198994.
Published: 9 September 2021
... and applications to lineage tracing in live-imaging data and outline software methodologies that can be applied to various cell-tracking challenges. Beyond the introduction and advancement of new microscopes capable of imaging large and sensitive specimens (of which there has been an explosion in recent years...
Journal Articles
Journal: Development
Development (2021) 148 (18): dev196121.
Published: 15 April 2021
... translational repression of zygotic hunchback mRNAs. Hunchback Drosophila embryo Translation SunTag Live imaging MS2 The gap gene hunchback ( hb ) is essential for body plan specification during Drosophila embryogenesis, primarily by establishing the gene expression boundaries...
Includes: Supplementary data
Journal Articles
Journal Articles
Journal: Development
Development (2019) 146 (4): dev169037.
Published: 15 February 2019
...-lethal activation of apoptotic signaling with the combination of a sensitive indicator for caspase 3 activation and in vivo live-imaging analysis of Drosophila . During thorax closure in pupal development, caspase 3 activation was specifically observed at the leading edge cells, with no signs...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2018) 145 (24): dev169797.
Published: 13 December 2018
...Huili Li; Jing Zhang; Lee Niswander ABSTRACT Micronutrition is essential for neural tube closure, and zinc deficiency is associated with human neural tube defects. Here, we modeled zinc deficiency in mouse embryos, and used live imaging and molecular studies to determine how zinc deficiency affects...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (23): 4322–4327.
Published: 1 December 2017
... and that this behavior depends upon mechanical forces. Quantitative live imaging and mathematical modeling allow us to correlate this tissue convergence with the underlying flow forces. We predict that tissue convergence is associated with the direction of the mean wall shear stress and of the gradient of harmonic phase...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (23): 4462–4472.
Published: 1 December 2017
.... Natl. Acad. Sci. USA   106 , 20812 - 20817 . 10.1073/pnas.0906464106 Sugiyama , M. , Saitou , T. , Kurokawa , H. , Sakaue-Sawano , A. , Imamura , T. , Miyawaki , A. and Iimura , T. ( 2014 ). Live imaging-based model selection reveals periodic regulation...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (20): 3766–3776.
Published: 15 October 2017
... morphological assessments of cellular rearrangements and time-lapse imaging to visualize cochlear remodeling in mouse. Analysis of cell redistribution showed that the cochlea extends through a combination of radial intercalation and cell growth. Live imaging demonstrated that concomitant cellular intercalation...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (20): 3840–3846.
Published: 15 October 2017
... and adult male gonads . Nat. Protoc.   1 , 1725 - 1731 . 10.1038/nprot.2006.235 Bardet , P.-L. , Kolahgar , G. , Mynett , A. , Miguel-Aliaga , I. , Briscoe , J. , Meier , P. and Vincent , J.-P. ( 2008 ). A fluorescent reporter of caspase activity for live imaging...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (13): 2249–2260.
Published: 1 July 2016
... vessels, and hence is fundamental for vascular network formation. Anastomosis can occur between two sprouts and involve two tip cells (‘head-to-head’ anastomosis), or between sprouts and a functional blood vessel, involving only one tip cell (‘head-to-side’ anastomosis) ( Fig. 1 ). Recent live-imaging...