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Keywords: Laser ablation
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Journal Articles
Journal: Development
Development (2022) 149 (16): dev200495.
Published: 22 August 2022
... for live imaging, and that this protein can be used for quantitative applications, such as FRAP or laser ablation. Finally, we believe that this fluorescent protein, with the set of new possibilities it offers, constitutes an important tool for cell, developmental and mechano-biologists in their current...
Includes: Supplementary data
Journal Articles
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Plant development
Journal: Development
Development (2021) 148 (18): dev198820.
Published: 16 June 2021
... not perturb polarity in Arabidopsis . (A) Schematic of the lateral ablations in VM. (B) Overview of representative VM before and after laser ablation. The growth of the meristem was followed for 5 days. The wounds are marked by yellow lightning signs and yellow arrowheads. The initial stages of leaf...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (2): dev195339.
Published: 26 January 2021
... is limited, additional complementary models would be useful. Here, we show that the ENS in zebrafish larvae at 10-15 days postfertilization is highly regenerative. After laser ablation, the number of enteric neurons recovered to ∼50% of the control by 10 days post-ablation (dpa). Using transgenic lines...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (7): 1350–1361.
Published: 1 April 2017
... density increased along the AP axis, thus relieving strain. We used laser ablation to isolate mesectoderm cells from the influence of other tissues. Uncoupling the mesectoderm from intercalating cells did not affect cell division orientation. Conversely, separating the mesectoderm from the anterior...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2015) 142 (11): 1992–2001.
Published: 1 June 2015
.... and Kuhlemeier , C. ( 2000 ). Auxin regulates the initiation and radial position of plant lateral organs . Plant Cell 12 , 507 - 518 . 10.1105/tpc.12.4.507 Reinhardt , D. , Frenz , M. , Mandel , T. and Kuhlemeier , C. ( 2003a ). Microsurgical and laser ablation analysis...
Journal Articles
Journal: Development
Development (2010) 137 (20): 3469–3476.
Published: 15 October 2010
... play a central role in establishing proper fates along the entire DV axis in this animal; laser ablation of midline cells causes a failure to form neurogenic ectoderm and Ph-sim RNAi results in severely dorsalized embryos lacking both neurogenic ectoderm and the appendage-bearing lateral ectoderm...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2005) 132 (1): 15–26.
Published: 1 January 2005
...-existing primordia influence further phyllotaxis. Here, we repeat these experiments and compare them with the results of laser ablation of incipient primordia. Furthermore, we explore to what extent the different pre-existing primordia influence the size and position of new organs, and hence phyllotaxis...
Journal Articles
Journal: Development
Development (2003) 130 (22): 5307–5318.
Published: 15 November 2003
... outgrowth of follower axons. Here, the dependencies between early and late outgrowing axons in the ventral cord were studied systematically with laser ablation experiments and a detailed analysis of mutants using multi-color GFP markers. Different classes of axon were affected to a different extent when...
Journal Articles
Journal: Development
Development (2003) 130 (17): 4073–4083.
Published: 1 September 2003
... of the stem cells. We have undertaken a series of high-precision laser ablation and microsurgical tissue removal experiments to test the functions of different parts of the tomato meristem, and to reveal their interactions. Ablation of the central zone led to ectopic expression of the WUSCHEL gene...
Journal Articles
Journal: Development
Development (2002) 129 (3): 617–624.
Published: 1 February 2002
... and dbd in a live specimen prior to laser ablation. (C) The same neurones as shown in B 12 hours after laser ablation of the four neurones ventral to dbd. One of the neurones is missing (*) and the other three neurones exhibit a condensed cell body, loss of dendrites and blebbing. (D) The same neurones 30...