... is specifically upregulated upon induction of the π lineage and not in response to other LIN-12/Notch-mediated decisions. We determined that dual regulation by LIN-12 and FOS-1 is required for egl-13 expression at specification and for complete rescue of egl-13 mutants. We found that fos-1 mutants exhibit uterine...

...Natalie de Souza; Laura G. Vallier; Hanna Fares; Iva Greenwald The vulval precursor cells (VPCs) of Caenorhabditis elegans are polarized epithelial cells that adopt a precise pattern of fates through regulated activity of basolateral LET-23/EGF receptor and apical LIN-12/Notch. During VPC...

...’ in the intestine. We have analyzed the development of twist to determine how left-right and anterior-posterior asymmetries are generated within the intestinal primordium. The twist requires the LIN-12/Notch-like signaling pathway of C. elegans . All cells within the intestinal primordium initially express LIN-12...

...Diane Levitan; Iva Greenwald ABSTRACT Presenilins have been implicated in the development of Alzheimer’s disease and in facilitating LIN-12/Notch activity. Here, we use genetic methods to explore the relationship between C. elegans LIN-12 and SEL-12 presenilin. Reducing sel-12 activity can suppress...

...Diane Levitan; Iva Greenwald ABSTRACT We have used a LIN-12::GFP fusion protein to examine LIN-12 accumulation during cell fate decisions important for vulval development. During the naturally variable anchor cell (AC)/ventral uterine precursor cell (VU) decision of the somatic gonad...

... cell) or later, just before the decision to commit to one of the three M-lineage fates (SM/bw/cc). Whenever CeMyoD acts, it is unlikely to act alone. At present, at least two other components affecting the hermaphrodite coelomocyte/sex myoblast decision are known. One of these components is LIN-12...

...Chenhui Wen; Mark M. Metzstein; Iva Greenwald ABSTRACT LIN-12/NOTCH proteins mediate cell-cell interactions that specify cell fates. Previous work suggested that sup-17 facilitates lin-12 signalling in Caenorhabditis elegans . Here, we show that sup-17 encodes a member of the ADAM family...

...Barth Grant; Iva Greenwald ABSTRACT Previous work indicated that sel-1 functions as a negative regulator of lin-12 activity, and predicted that SEL-1 is a secreted or membrane associated protein. In this study, we describe cell ablation experiments that suggest sel-1 mutations elevate lin-12...

...Ivan P. G. Moskowitz; Joel H. Rothman ABSTRACT Cell-cell interactions mediated by LIN-12 and GLP-1, members of the LNG (LIN-12, Notch, GLP-1) family of receptors, are required to specify numerous cell fates during development of the nematode Caenorhabditis elegans . Maternally expressed GLP-1...

...Sioux Christensen; Voula Kodoyianni; Marcus Bosenberg; Lisa Friedman; Judith Kimble ABSTRACT The homologous receptors LIN-12 and GLP-1 mediate diverse cell-signaling events during development of the nematode Caenorhabditis elegans . These two receptors appear to be functionally interchangeable...

...Kevin Fitzgerald; Iva Greenwald ABSTRACT Ligands of the Delta/Serrate/lag-2 (DSL) family and their receptors, members of the lin-12/Notch family, mediate cell-cell interactions that specify cell fate in invertebrates and vertebrates. In C. elegans , two DSL genes, lag-2 and apx-1 , influence...

...Anna P. Newman; John G. White; Paul W. Sternberg ABSTRACT The anchor cell (AC) of the Caenorhabditis elegans gonad has a critical role in the development of a functional egglaying system, which is accomplished through cell-cell interactions. Lateral inhibitory lin-12 -mediated signaling among two...

...Samuel T. Henderson; Dali Gao; Eric J. Lambie; Judith Kimble ABSTRACT The C. elegans lag-2 gene is required for several cell-cell interactions that rely on the receptors GLP-1 and LIN-12. In this paper, we report that lag-2 encodes a putative membrane protein with sequence similarity to Drosophila...

...Kevin Fitzgerald; Hilary A. Wilkinson; Iva Greenwald ABSTRACT Members of the lin-12/Notch gene family encode receptors for intercellular signals and are found throughout the animal kingdom. In many animals, the presence of at least two lin-12/Notch genes raises the issue of the significance...

...Eric J. Lambie; Judith Kimble ABSTRACT Two homologous genes, lin-12 and glp-1 , encode transmembrane proteins required for regulatory cell interactions during C. elegans development. Based on their single mutant phenotypes, each gene has been thought to govern a distinct set of cell fates. We show...

...Judith Austin; Eleanor M. Maine; Judith Kimble ABSTRACT Cell-cell interactions play a significant role in controlling cell fate during development of the nematode Caenorhabditis elegans . It has been found that two genes, glp-1 and lin-12 , are required for many of these decisions, glp-1...