...Daniel Castranova; Bakary Samasa; Marina Venero Galanternik; Aniket V. Gore; Allison E. Goldstein; Jong S. Park; Brant M. Weinstein ABSTRACT The zebrafish has become a widely used animal model due, in large part, to its accessibility to and usefulness for high-resolution optical imaging. Although...

...Simon Berger; Silvan Spiri; Andrew deMello; Alex Hajnal ABSTRACT Several microfluidic-based methods for Caenorhabditis elegans imaging have recently been introduced. Existing methods either permit imaging across multiple larval stages without maintaining a stable worm orientation, or allow for very...

.... , Lamprecht , M. R. , Clarke , C. , Kang , I. H. , Friman , O. , Guertin , D. A. , Chang , J. H. , Lindquist , R. A. , Moffat , J. , Golland , P. and Sabatini , D. M. ( 2006 ). CellProfiler: image analysis software for identifying and quantifying cell phenotypes...

... expression in gpc4 mutants at least partially contributes to defects in endodermal migration. Fig. 6. Endodermal defects in gpc4 mutants are suppressed by knocking down fn1a and lamb1a . (A-D) Epifluorescence still images of the anterior region of the endodermal sheet in 6s embryos derived...

... and used to define stages. With the advent of high-throughput 3D imaging tools that capture embryo morphology in microscopic detail, we now present the first 4D atlas staging system for mouse embryonic development using optical projection tomography and image registration methods. By tracking 3D...

...-cell level. Endothelium Imaging RUNX1 SOX17 Mice Human Hemogenic Hematopoiesis Endothelial-to-hematopoietic transition Scanning electron microscopy Endothelial-to-hematopoietic transition (EHT) during embryogenesis provides the first long-term hematopoietic stem and progenitor...

... intravital dye labeling and 3D imaging, we discovered that systems-level vascular patterning in the kidney is dependent on the kinetics of vascular mural cell (VMC) differentiation. Conditional ablation of the TALE transcription factor Pbx1 in renal VMC progenitors in the mouse led to the premature...

... expressed separately. The proteins indicated at the top of each image were expressed in salivary glands. The loci that were bound by the fusion proteins were visualized by immunostaining (shown in yellow) using the antibodies indicated. Loci that were bound predominantly by co-expressed dKeap1 and CncC...

.... The IMPC has selected three-dimensional (3D) imaging to phenotype these mouse lines at relevant stages of embryonic development in an attempt to discover the cause of lethality using detailed anatomical information. Rate of throughput is paramount as IMPC production centers have been given the ambitious...

..., distribution and reproduction in any medium provided that the original work is properly attributed. Imaging Chromosomes Meiosis Mouse Oocyte In oocytes, chromosome mis-segregation is common and generates aneuploid embryos, so causing lowered implantation rates, pregnancy loss...

..., for generating the transgenic lines that form the basis of this manuscript; L. Huitema, A. van Impel, I. Logister and B. Ponsioen for providing images for Fig. 3 ; and Josi Peterson-Maduro and Ive Logister for many improvements to the protocol. J.B. is grateful to Arndt Siekmann for support. References...

... capacity in animals, and the potential for differentiated melanocyte cell division in development and regeneration remains largely unexplored. Here, we use time-lapse imaging of the developing zebrafish to show that while most melanocytes arise from undifferentiated precursor cells, an unexpected...