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Keywords: Genome editing
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Journal Articles
Journal: Development
Development (2021) 148 (19): dev199536.
Published: 13 October 2021
... maternal crispants enables the identification of maternal-effect genes in a single generation. Crispr Maternal effect Genome editing Early development Zebrafish Kpna7 Fhdc3 National Institutes of Health http://dx.doi.org/10.13039/100000002 GM065303 In zebrafish...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (7): dev193565.
Published: 15 April 2021
... of subsequent effects on cell behavior. Here, we present a single-plasmid approach for genome editing in chick embryos to study experimentally perturbed cells in an otherwise normal embryonic environment. To achieve this, we have engineered a plasmid that encodes Cas9 protein, gene-specific guide RNA (gRNA...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2020) 147 (19): dev193029.
Published: 12 October 2020
.... Y. , Fu , Y. , Reyon , D. , Maeder , M. L. , Tsai , S. Q. , Sander , J. D. , Peterson , R. T. , Yeh , J. R. and Joung , J. K . ( 2013 ). Efficient genome editing in zebrafish using a CRISPR-Cas system . Nat. Biotechnol.   31 , 227 - 229 . 10.1038/nbt.2501...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (13): 2428–2444.
Published: 01 July 2017
... enhancer elements near these target genes. Functional experiments performed in vitro and in vivo confirm that this network of genes requires ERK, ERG and p300 activity. Finally, genome-editing and transgenic approaches demonstrate that a highly conserved ERG-bound enhancer located upstream of HLX (which...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (4): 635–648.
Published: 15 February 2017
... ); and (4) engineering of glioma mutations ( TP53 deletion; H3F3A point mutations). These resources and optimised methods enable facile and scalable genome editing in mammalian NSCs, providing significant new opportunities for functional genetic analysis. To demonstrate possibilities for loss-of-function...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (1): 3–7.
Published: 01 January 2017
... technological advances in single-cell analysis, allowing in-depth analysis of the human embryo. This field could shortly gain more momentum as novel genome editing technologies might, for the first time, also allow functional genetic studies in the human embryo. In this Spotlight article, we summarize...
Journal Articles
Journal: Development
Development (2016) 143 (22): 4097–4100.
Published: 15 November 2016
... Stem Cell Symposium this year. Bioinformatics Computational biology Epigenetics Genome editing Stem cells Transcription Quantitative approaches have been boosted across many areas of biology by the relentless improvements in sequencing, imaging, computational methods...
Journal Articles
Journal: Development
Development (2016) 143 (15): 2868–2875.
Published: 01 August 2016
...Ira L. Blitz; Margaret B. Fish; Ken W. Y. Cho CRISPR/Cas9 genome editing is revolutionizing genetic loss-of-function analysis but technical limitations remain that slow progress when creating mutant lines. First, in conventional genetic breeding schemes, mosaic founder animals carrying mutant...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (13): 2344–2355.
Published: 01 July 2016
.... Using CRISPR/Cas9-mediated genome editing, we generated and analyzed developmental defects in mice carrying additional Golgb1 loss-of-function mutations, which supported a crucial requirement for Golgb1 in palate development. Through maxillary explant culture assays, we demonstrate that the Golgb1...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (11): 2025–2037.
Published: 01 June 2016
... Mutagenesis Genome editing RNP CrispantCal CrispRVariants Cas9 nuclease-mediated mutagenesis through non-homologous end joining (NHEJ) repair enables rapid, site-directed mutagenesis of candidate genes in zebrafish for somatic as well as stable germline mutant analysis ( Chang et al., 2013...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (24): 4827–4830.
Published: 15 December 2014
...Uwe Irion; Jana Krauss; Christiane Nüsslein-Volhard The introduction of engineered site-specific DNA endonucleases has brought precise genome editing in many model organisms and human cells into the realm of possibility. In zebrafish, loss-of-function alleles have been successfully produced...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (21): 4115–4120.
Published: 01 November 2014
... ( albertostolfi@gmail.com ; lc121@nyu.edu ) Competing interests The authors declare no competing financial interests. 18 6 2014 1 9 2014 © 2014. Published by The Company of Biologists Ltd 2014 Ascidians CRISPR/Cas9 Ciona Ebf Genome editing Islet Recent advances have...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (17): 3331–3339.
Published: 01 September 2014
... the activation of the Shh response ( Corbit et al., 2005 ; Huangfu and Anderson, 2005 ; Rohatgi et al., 2007 ). This model explains the widespread activation of the Shh response observed in the absence of Ptch1 ( Goodrich et al., 1997 ). Patched1 Patched2 Shh Genome editing Mouse embryonic stem...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (3): 707–714.
Published: 01 February 2014
... nucleases (TALENs), which require either complex design and selection or laborious construction. Thus, easy and efficient genome editing tools are still highly desirable for this species. Here, we report that RNA-guided Cas9 nuclease resulted in precise targeted gene disruption in all ten X. tropicalis...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2013) 140 (24): 4982–4987.
Published: 15 December 2013
...Alexander Hruscha; Peter Krawitz; Alexandra Rechenberg; Verena Heinrich; Jochen Hecht; Christian Haass; Bettina Schmid Gene modifications in animal models have been greatly facilitated through the application of targeted genome editing tools. The prokaryotic CRISPR/Cas9 type II genome editing...
Includes: Supplementary data