... for live imaging, and that this protein can be used for quantitative applications, such as FRAP or laser ablation. Finally, we believe that this fluorescent protein, with the set of new possibilities it offers, constitutes an important tool for cell, developmental and mechano-biologists in their current...

.../user-licence-1-1/ Highlighted Article: FRAP and optogenetic experiments reveal that Lgl dynamics in the lateral cortex of epithelial cells do not require binding to Scrib-Dlg, but depend on their ability to limit aPKC activity. Epithelial cell Apical-basal polarity Basolateral...

... germline morphogens, but also somatic determinants. * Author for correspondence ( wirtz@jhu.edu ) Competing interests statement 18 5 2010 © 2010. C. elegans FCS FRAP MEX-5 Live-cell microscopy Protein diffusion A common mechanism of generating...

...Gabriella Ficz; Rainer Heintzmann; Donna J. Arndt-Jovin Fluorescence recovery after photobleaching (FRAP) microscopy was used to determine the kinetic properties of Polycomb group (PcG) proteins in whole living Drosophila organisms (embryos) and tissues (wing imaginal discs and salivary glands...