... contexts. Loss of NMD factors leads to embryonic lethality in organisms spanning the phylogenetic scale, but the mechanism remains unknown. Here, we report that the core NMD factor, UPF2, is required for expansion of epiblast cells within the inner cell mass of mice in vivo . We identify NMD target mRNAs...

... embryogenesis. These complex tasks are resolved through an intricate combination of signals controlling the induction of extraembryonic lineages and, at the same time, safeguarding the pluripotent epiblast. Here, we delineate the signals orchestrating primate epiblast and amnion identity. We encapsulated...

...Koya Yoshihi; Kagayaki Kato; Hideaki Iida; Machiko Teramoto; Akihito Kawamura; Yusaku Watanabe; Mitsuo Nunome; Mikiharu Nakano; Yoichi Matsuda; Yuki Sato; Hidenobu Mizuno; Takuji Iwasato; Yasuo Ishii; Hisato Kondoh ABSTRACT Despite previous intensive investigations on epiblast cell migration...

... differentiation was evident in teratomas and readily induced in vitro . Global transcriptome analyses highlighted commonality in transcription factor expression across the three species, while global comparison with porcine embryo stages showed proximity to bilaminar disc epiblast. Clonal genetic manipulation...

... conservation observed. Single-cell transcriptomics analysis of human embryos previously enabled us to identify transcription factors, including the zinc-finger protein KLF17, that are enriched in the human epiblast and naïve human embryonic stem cells (hESCs). Here, we show that KLF17 is expressed coincident...

... that enable both robust cell-type proportioning despite variable initial conditions in the precursor cells, and the re-establishment of these proportions upon perturbations in a developing tissue remain to be characterized. Here, we report that the differentiation of robust proportions of epiblast-like...

... Self-organisation Epiblast Primitive endoderm One of the most fundamental questions in biology is: how can one cell, a fertilised zygote, give rise to all of the different cell types found in the adult body? The sequential formation of different types of cells that arrange themselves...

...Sofiane Hamidi; Yukiko Nakaya; Hiroki Nagai; Cantas Alev; Takeya Kasukawa; Sapna Chhabra; Ruda Lee; Hitoshi Niwa; Aryeh Warmflash; Tatsuo Shibata; Guojun Sheng ABSTRACT The pluripotent epiblast gives rise to all tissues and organs in the adult body. Its differentiation starts at gastrulation, when...

...Maria Rostovskaya; Giuliano G. Stirparo; Austin Smith ABSTRACT Human naïve pluripotent stem cells (PSCs) share features with the pre-implantation epiblast. They therefore provide an unmatched opportunity for characterising the developmental programme of pluripotency in Homo sapiens . Here, we...

... these studies, which suggest that the posterior epiblast and/or the nascent amnion in pregastrulation human embryos is a likely source of hPGCs, and that a different gene regulatory network controls PGCs in humans compared with in the mouse. Such studies on the origins and mechanisms of hPGC specification...

... a conditional deletion system to investigate embryonic patterning and lineage specification in response to loss of Oct4. We first observe ectopic expression of Nanog in Oct4-negative postimplantation epiblast cells. The expression domains of lineage markers are subsequently disrupted. Definitive endoderm...

...Anna Piliszek; Zofia E. Madeja; Berenika Plusa Formation of epiblast (EPI) – the founder line of all embryonic lineages – and extra-embryonic supportive tissues is one of the key events in mammalian development. The prevailing model of early mammalian development is based almost exclusively...

... to the pluripotent epiblast (EPI), which forms the embryo proper, and the primitive endoderm (PrE), which forms extra-embryonic yolk sac tissues. All inner cells coexpress lineage markers such as Nanog and Gata6 at embryonic day (E) 3.25, and the EPI and PrE precursor cells eventually segregate to exclusively...

...Mubeen Goolam; Magdalena Zernicka-Goetz The separation of embryonic from extra-embryonic tissues within the inner cell mass to generate the epiblast (EPI), which will form the new organism, from the primitive endoderm (PE), which will form the yolk sac, is a crucial developmental decision. Here, we...

... of early post-implantation epiblast and are distinct from primed epiblast. They also exhibit a genome-wide increase in DNA methylation, intermediate between early and late epiblast. These findings are consistent with the proposition that naïve cells transition to a distinct formative phase of pluripotency...

... and trophectoderm do not originate from the epiblast during embryo development, and although some reports suggest they may be generated from ESCs in vitro ( Morgani et al., 2013 ), this would not proceed via the formative phase. During the formative interval, pluripotent cells have lost naïve identity...

...Thorsten Boroviak; Jennifer Nichols ABSTRACT Naïve pluripotent mouse embryonic stem cells (ESCs) resemble the preimplantation epiblast and efficiently contribute to chimaeras. Primate ESCs correspond to the postimplantation embryo and fail to resume development in chimaeric assays. Recent data...

...Audrey Laurent; Francesco Blasi Between implantation and gastrulation, mouse pluripotent epiblast cells expand enormously in number and exhibit a remarkable hypersensitivity to DNA damage. Upon low-dose irradiation, they undergo mitotic arrest followed by p53-dependent apoptosis, whereas the other...

... expressed in the human pluripotent epiblast, including the transcription factor KLF17 . Key components of the TGF-β signalling pathway, including NODAL , GDF3 , TGFBR1/ALK5 , LEFTY1 , SMAD2 , SMAD4 and TDGF1 , are also enriched in the human epiblast. Intriguingly, inhibition of TGF-β signalling abrogates...

...Sylvain Bessonnard; Laurane De Mot; Didier Gonze; Manon Barriol; Cynthia Dennis; Albert Goldbeter; Geneviève Dupont; Claire Chazaud During blastocyst formation, inner cell mass (ICM) cells differentiate into either epiblast (Epi) or primitive endoderm (PrE) cells, labeled by Nanog and Gata6...