... membrane play an important role in the proper elongation of the developing egg chamber; however, the molecular mechanisms contributing to basement membrane mechanical properties are not fully understood. Here, we systematically analyze the contributions of individual ECM components towards the molecular...

...Darcy Andersen; Sally Horne-Badovinac Organs are formed from multiple cell types that make distinct contributions to their shape. The Drosophila egg chamber provides a tractable model to dissect such contributions during morphogenesis. Egg chambers consist of 16 germ cells (GCs) surrounded...

... is determined by the expression of the pipe gene in a subpopulation of ventral follicle cells in the developing egg chamber. The Pipe protein exhibits homology to vertebrate glycosaminoglycan sulfotransferases. In a previous study, we demonstrated that embryonic DV polarity depends upon the sulfotransferase...

...-proteasome. These complexes are composed of three constant polypeptides – Skp1, Cullin1/3 and Roc1/Rbx1– and a fourth variable adapter, the F-box protein. Slimb (Slmb) is a Drosophila F-Box protein that fulfills several roles in development and cell physiology. We analyzed its participation in egg chamber...

... cells into apoptosis( Prendergast, 1999 ). Fig. 7. Growth of germline and somatic tissues in the ovary is tightly coordinated.(A,B) An egg chamber with a dm 2 mutant germline(arrowhead) exhibits abnormal perdurance of Fas III expression in the overlying wild-type follicle cells. (C,D...

.... In this process, dome controls the expression of the apical determinant Crumbs. In contrast to the ligand Upd, whose expression is limited to a pair of polar cells at both ends of the egg chamber, dome is expressed in all germline and follicle cells. However, the Dome protein is specifically localized...

... egg chambers appeared to have fng + polar cells ( Fig. 5 A). These follicles exhibit a consistent defect in which the epithelium is incomplete at the termini next to the fng − stalk cells, leaving some germline cells uncovered by follicle cells ( Fig. 5 A). Since this gap in the follicle...

... to the developing oocyte. Analysis of Src64 egg chambers showed defects in the ring canals that interconnect the oocyte and its 15 associated nurse cells. Src64 ring canals fail to accumulate the high levels of tyrosine phosphorylation that are normally present. Despite the reduced tyrosine phosphorylation, known...

... cytoskeleton axis formation RNA localization Bicaudal-D ( Bic-D ) egg chamber follicle cell nurse cell Asymmetric localization of patterning factors within cells often underlies developmental patterning, and Drosophila oogenesis has proven to be a valuable system for studying how...

... depleted in larvae, the resulting adult phenotypes demonstrate that RLC is required in a stage-specific fashion for proper development of eye and leg imaginal discs. When RLC is depleted in adult females, oogenesis is reversibly disrupted. Without RLC induction, developing egg chambers display a succession...