... of the third instar. This change in barrier permeability is driven by the steroid hormone ecdysone and correlates with changes in localization of Coracle, a component of the septate junctions that is required for the late-larval impermeable epithelial barrier. Based on these observations, we propose...

... replacement ( Tettamanti and Casartelli, 2019 ). Intensive studies based on the larva-pupa transition of D . melanogaster have demonstrated the determinant role of ecdysone signaling in PCD processes that affect the salivary glands, midgut and fat body. Ecdysone, or rather its most bioactive derivative 20...

... identify the Ecdysone Receptor (EcR) in the PTTH-expressing neurons (PTTHn) as a regulator of developmental maturation onset. Loss of EcR in these PTTHn impairs PTTH signaling, which delays maturation. We find that the steroid ecdysone dose-dependently affects Ptth transcription, promoting its expression...

... in the same cells to respond to spatial cues through regulation of chromatin accessibility. Temporal transcription factor Chromatin accessibility Enhancer Gene regulation Ecdysone Drosophila Cis -regulatory regions such as enhancers and promoters interpret multiple types of inputs...

...Coralie Drelon; Michael F. Rogers; Helen M. Belalcazar; Julie Secombe ABSTRACT In Drosophila , the larval prothoracic gland integrates nutritional status with developmental signals to regulate growth and maturation through the secretion of the steroid hormone ecdysone. While the nutritional signals...

... to pupal stages but the expression of Fos is specifically activated by ecdysone receptor B1 (EcRB1) at early pupal stages, suggesting that ecdysone signaling provides temporal control of the regulation of dendrite pruning by JNK signaling. Thus, our work not only identifies a novel pathway involved...

... studies. Of note, even among these studies, there are considerable differences in the reported phenotypes between groups and publications ( Grönke et al., 2010 ; Ikeya et al., 2002 ; Rulifson et al., 2002 ; Zhang et al., 2009 ). To further define the role of ecdysone signalling in IPCs, we first...

...H. Frederik Nijhout; Emily Laub; Laura W. Grunert ABSTRACT The wing imaginal disks of Lepidoptera can be grown in tissue culture, but require both insulin and ecdysone to grow normally. Here, we investigate the contributions the two hormones make to growth. Ecdysone is required to maintain mitoses...

... by the duration of this phase, which is under neuroendocrine control. The neuropeptide prothoracicotropic hormone (PTTH) has been proposed to play a central role in controlling the length of the larval phase through regulation of ecdysone production, a steroid hormone that initiates larval molting...

... in the optic lobe during early larval stages by boosting symmetric self-renewing divisions while preventing differentiation. Neuroepithelium differentiation in late larvae requires the transcriptional silencing of chinmo by ecdysone, the main steroid hormone, therefore allowing coordination of neural stem cell...

...-licence-1-1/ Summary: Identification of a requirement for 20-hydroxyecdysone in tissue patterning leads to the discovery that dynamic cell rearrangements and shape changes contribute to oriented tissue growth in the Drosophila wing disc. Ecdysone Insulin Culture Imaginal disc...

... - with multiple cis -regulatory elements integrating nutritional and tissue-specific inputs. dFOXO Ecdysone Insulin receptor Retinoblastoma Transcriptional enhancer The insulin signaling pathway plays essential roles in growth and metabolism in metazoans. In mammals, the insulin receptor (INSR...

... timing is believed to be determined by the release of a steroid hormone, ecdysone (E), from the prothoracic gland. Here, we demonstrate that the ecdysone-20-monooxygenase Shade determines pupation timing by converting E to 20-hydroxyecdysone (20E) in the fat body, which is the organ that senses...

... LC-MS/MS Dietary sterols Ecdysone Ecdysteroids Makisterone A Drosophila melanogaster is emerging as a powerful model in which to study hormonal control of growth, metabolism and development (reviewed by Padmanabha and Baker, 2014 ; Shim et al., 2013 ; Tennessen and Thummel, 2011...

... and germ cell differentiation, and promotes the accumulation of Broad-Z1, which is also a target of the steroid hormone ecdysone. Epistasis analysis shows that Activin controls cell proliferation in an ecdysone-independent manner and TF differentiation by affecting ecdysone targets. We propose...

... to fine-tune the dynamics of hormone production. In Drosophila , transient increases of the steroid prohormone ecdysone, produced at each larval stage, are necessary to trigger moulting and metamorphosis. Binding of the active ecdysone (20-hydroxyecdysone) to its receptor (EcR) is followed...

... coordinate growth and timing of maturation during development, the mechanisms generating these pulses are not known. Here we show that the ecdysone steroid pulse that drives the juvenile-adult transition in Drosophila is determined by feedback circuits in the prothoracic gland (PG), the major steroid...

... that activin signaling regulates competence of the prothoracic gland to receive PTTH and insulin signals, and that these two pathways act at the mRNA and post-transcriptional levels, respectively, to control ecdysone biosynthetic enzyme expression. This dual regulatory circuitry may provide a cross-check...

.... Why do larvae arrest growth upon dietary sterol depletion? One possibility is that growth requires normal levels of membrane sterol. Another possibility is that sterol depletion might reduce sterol levels below a threshold required for ecdysone biosynthesis. Pulses of the steroid hormone ecdysone...

... guided by a principal steroid hormone, ecdysone. Among the multiple conversion steps for synthesizing ecdysone from dietary cholesterol, the conversion of 7-dehydrocholesterol to 5β-ketodiol, the so-called ‘Black Box’, is thought to be the important rate-limiting step. Although a number of genes...