...Albert Tsai; Justin Crocker ABSTRACT An embryo experiences increasingly complex spatial and temporal patterns of gene expression as it matures, guiding the morphogenesis of its body. Using super-resolution fluorescence microscopy in Drosophila melanogaster embryos, we observed that the nuclear...

...) #24749]. All flies used were strains of Drosophila melanogaster and reared at 25°C on standard fly media. See Table S1 for details regarding stocks used. Our observation of increased pMad + cell number in germline-specific brk mutants or RNAi was surprising given our findings...

... in vivo imaging Cell tracking Drosophila melanogaster National Institutes of Health http://dx.doi.org/10.13039/100000002 R35GM118146 American Heart Association http://dx.doi.org/10.13039/100000968 18POST34080493 California Institute Of Technology https...

.../eLife.28275.026 Harrison , M. M. , Li , X.-Y. , Kaplan , T. , Botchan , M. R. and Eisen , M. B. ( 2011 ). Zelda binding in the early Drosophila melanogaster embryo marks regions subsequently activated at the maternal-to-zygotic transition . PLoS Genet. 7 , e1002266...

... veins . Annu. Rev. Genet.   1940 , 28 . Grieder , N. C. , Morata , G. , Affolter , M. and Gehring , W. J. ( 2009 ). Spalt major controls the development of the notum and of wing hinge primordia of the Drosophila melanogaster wing imaginal disc . Dev. Biol.   329 , 315 - 326...

... tyrosine kinase in gut development in Drosophila melanogaster . EMBO Rep.   4 , 781 - 786 . 10.1038/sj.embor.embor897 Lu , X. , Chou , T. B. , Williams , N. G. , Roberts , T. and Perrimon , N. ( 1993 ). Control of cell fate determination by p21ras/Ras1, an essential...

... by guidance signals towards somatic gonadal precursor cells (SGPs). In Drosophila melanogaster , SGP-derived Hedgehog (Hh), which serves as a guidance cue for the PGCs, is potentiated by mesodermally restricted HMGCoA-reductase (Hmgcr) and the ABC transporter Multi-drug-resistant-49 (Mdr49). Given...

... development. Drosophila melanogaster Embryogenesis lncRNA Spatiotemporal transcriptome Neurogenesis Development of complex tissues from naïve primordia requires the precise spatiotemporal deployment of transcriptional programs as cells subdivide, specify and differentiate. Owing...

...-1/ Summary: In Drosophila , neuroblasts and cortex glia use a receptor tyrosine kinase-dependent feed-forward mechanism to actively maintain each other during neurogenesis, which contributes to glial tumorigenesis. Niche Glia Neuroblast PDGFR Drosophila melanogaster...

..., 2013 ). Drosophila melanogaster Epithelial-to-mesenchymal (EMT) Mesenchymal-to-epithelial (MET) Fibroblast growth factor (FGF) Adherens junction Integrin Bazooka Summary: FGF regulates epithelial-mesenchymal transition in mesoderm to support collective movement at gastrulation...

... requirements for effective Hh shedding remained undefined. In this work, by using Drosophila melanogaster as a model, we show that mutagenesis of the N-terminal Cardin–Weintraub (CW) motif inactivates recombinant Hh proteins to variable degrees and, if overexpressed in the same compartment, converts them...

... PNS during early pupal phase. Dendrite Pruning Microtubule Kinesin Plus end Drosophila melanogaster The physiological degeneration of synapses, axons or dendrites without loss of the parent neuron is known as pruning. Pruning is an important developmental mechanism that is used...

... Drosophila melanogaster Polycomb silencing is essential for the developmental regulation of gene expression ( Grossniklaus and Paro, 2014 ; Comet et al., 2016 ; Kundu et al., 2017 ). The silencing needs to be robust to tightly repress the expression of developmental genes in undifferentiated cells...

... transients modulate the temporal dynamics of Dpp presentation in developmental BMP signaling, impacting the downstream transcriptional response and tissue morphogenesis. Fly wing morphogenesis Dpp BMP Bioelectric signaling KCNJ2 Drosophila melanogaster Dominant-negative mutations...

... a cullin-mediated EGFR-dependent mechanism and by an alternative route. Capicua COP9 signalosome Drosophila melanogaster EGFR Nedd8 Ubiquitin ligase Wing disc Regulation of protein stability is an important feature of many signaling pathways. Crucial pathway components can...

.... Wingless Wnt Shavenbaby Ovo SoxNeuro Denticle Morphogenesis Drosophila melanogaster Information from signaling pathways must be interpreted and modulated for the proper execution of cellular events during development. Much of developmental biology research has focused on the signaling...

... is essential for efficient pre-mRNA splicing. Drosophila melanogaster Tracheal system Branching morphogenesis mRNA splicing Spliceosome Faint sausage Fandango mRNA splicing is required to process nearly all eukaryotic transcripts. As splicing can be rate-limiting for efficient gene...

... Jaglarz , M. K. and Howard , K. R. ( 1994 ). Primordial germ cell migration in Drosophila melanogaster is controlled by somatic tissue . Development   120 , 83 - 89 . Jaglarz , M. K. and Howard , K. R. ( 1995 ). The active migration of Drosophila primordial germ cells...

...Girish Deshpande; Diane Manry; Nicholas Jourjine; Vladic Mogila; Henny Mozes; Tzofia Bialistoky; Offer Gerlitz; Paul Schedl Coalescence of the embryonic gonad in Drosophila melanogaster requires directed migration of primordial germ cells (PGCs) towards somatic gonadal precursor cells (SGPs...

... ). How one becomes many: blastoderm cellularization in Drosophila melanogaster . Bioessays   24 , 1012 - 1022 . 10.1002/bies.10184 Millard , T. H. and Martin , P. ( 2008 ). Dynamic analysis of filopodial interactions during the zippering phase of Drosophila dorsal closure...