...Anwesha Guru; Surat Saravanan; Deepanshu Sharma; Maithreyi Narasimha ABSTRACT Apical constriction powers amnioserosa contraction during Drosophila dorsal closure. The nucleation, movement and dispersal of apicomedial actomyosin complexes generates pulsed apical constrictions during early closure...

..., embryonic dorsal closure and pupal thorax closure in Drosophila are useful experimental models. We find that Pvr mutants have defects in dorsal midline closure with incomplete amnioserosa internalization and epidermal zippering, as well as cardia bifida. These defects are relatively mild in comparison...

...Ginger L. Hunter; Janice M. Crawford; Julian Z. Genkins; Daniel P. Kiehart We demonstrate that ion channels contribute to the regulation of dorsal closure in Drosophila , a model system for cell sheet morphogenesis. We find that Ca 2+ is sufficient to cause cell contraction in dorsal closure...

...Raphaël Rousset; Sophie Bono-Lauriol; Melanie Gettings; Magali Suzanne; Pauline Spéder; Stéphane Noselli In Drosophila melanogaster , dorsal closure is a model of tissue morphogenesis leading to the dorsal migration and sealing of the embryonic ectoderm. The activation of the JNK signal...

...Sami Bahri; Simon Wang; Ryan Conder; Juliana Choy; Stephanie Vlachos; Kevin Dong; Carlos Merino; Stephan Sigrist; Cristina Molnar; Xiaohang Yang; Edward Manser; Nicholas Harden Dorsal closure (DC) of the Drosophila embryo is a model for the study of wound healing and developmental epithelial...

... of deafness in mammals. Here we show that the Drosophila myosin XV homolog, Sisyphus, is expressed at high levels in leading edge cells and their cellular protrusions during the morphogenetic process of dorsal closure. Sisyphus is required for the correct alignment of cells on opposing sides of the fusing...

..., we show that Drosophila Fer localises to adherens junctions in the dorsal epidermis and regulates a major morphological event, dorsal closure. Mutations in Src42A cause defects in dorsal closure similar to those seen in dfer mutant embryos. Furthermore, Src42A mutations enhance the dfer mutant...

... between the two cell types of the dorsal appendage primordia. In the embryo, Ed is absent from the amnioserosa during dorsal closure,generating an Ed expression border with the lateral epidermis that coincides with the actomyosin cable present at this interface. In both cases, ed mutant epithelia exhibit...

...Romi Biswas; David Stein; E. Richard Stanley Embryonic dorsal closure (DC) in Drosophila is a series of morphogenetic movements involving the bilateral dorsal movement of the epidermis (cell stretching) and dorsal suturing of the leading edge (LE) cells to enclose the viscera. The Syk family...

... that Src42A is essential for proper cell-cell matching during dorsal closure. Src42A, which is functionally redundant to Src64 , was found to interact genetically with shotgun , a gene encoding E-cadherin, and armadillo , a Drosophila β-catenin. Immunoprecipitation and a pull-down assay indicated that Src42A...

...Véronique Morel; Alfonso Martinez Arias At the end of germband retraction, the dorsal epidermis of the Drosophila embryo exhibits a discontinuity that is covered by the amnioserosa. The process of dorsal closure (DC) involves a coordinated set of cell-shape changes within the epidermis...

... molecularly characterized the Drosophila homologue of MYPT, and analyzed its mutant phenotypes. We find that Drosophila MYPT is required for cell sheet movement during dorsal closure, morphogenesis of the eye, and ring canal growth during oogenesis. Our results indicate that the regulation...

...James W. Bloor; Daniel P. Kiehart The small GTPase Rho is a molecular switch that is best known for its role in regulating the actomyosin cytoskeleton. We have investigated its role in the developing Drosophila embryonic epidermis during the process of dorsal closure. By expressing the dominant...

... implicated in dorsal closure and is not mediated by the JNK pathway, which is also necessary for Dpp activity in these cells. In addition, we show that pnr behaves as a selector-like gene in generating morphological diversity in the dorsoventral body axis. It is responsible for maintaining a subdivision...

...Beth E. Stronach; Norbert Perrimon The leading edge (LE) is a single row of cells in the Drosophila embryonic epidermis that marks the boundary between two fields of cells: the amnioserosa and the dorsal ectoderm. LE cells play a crucial role in the morphogenetic process of dorsal closure...

... during dorsal closure, while Wingless signaling regulates segmental patterning of the larval cuticle via Armadillo. wingless or armadillo mutant embryos secrete a lawn of ventral denticles; armadillo mutants also exhibit dorsal closure defects. We found that mutations in puckered , a phosphatase...

... severe defects in head involution and imperfect dorsal closure. Two phenotypes are associated with reduction of maternal Rho1 activity: the actin cytoskeleton is disrupted in egg chambers, especially in the ring canals and embryos display patterning defects as a result of improper maintenance...

...Cherie Lynne Byars; Katherine L. Bates; Anthea Letsou During dorsal closure in Drosophila melanogaster , cells of the lateral epidermis migrate over the amnioserosa to encase the embryo. At least three classes of dorsal-open group gene products are necessary for this morphogenetic movement. Class I...

...Julia Zeitlinger; Dirk Bohmann* Dorsal closure, a morphogenetic movement during Drosophila embryogenesis, is controlled by the Drosophila JNK pathway, D-Fos and the phosphatase Puckered (Puc). To identify principles of epithelial closure processes, we studied another cell sheet movement that we...

... dominant inhibitory ( N17DRacA ), and wild-type versions of the DRacA cDNA under control of an Hsp70 promoter. Expression of the N17DRacA transgene during embryonic development causes a high frequency of defects in dorsal closure which are due to disruption of cell shape changes in the lateral epidermis...