... for multiple rounds of DNA replication, likely via endocycles, in trophoblast giant cells. Furthermore, MYC supports the expression of DNA replication and nucleotide biosynthesis genes along with ribosomal RNA. Increased DNA damage and senescence occur in trophoblast giant cells without Myc , accompanied...

... at the two-cell stage regulates asynchronous cell cycling. Caenorhabditis   elegans Early embryo Cell cycle CYB-3 cyclin Asynchrony DNA replication How cell cycle control pathways are regulated during early embryogenesis and the means by which cell cycle timing impacts development...

... zebrafish have global DNA hypomethylation and display embryonic defects, including a small liver, and they die as larvae. We make the surprising finding that, despite their reduced organ size, uhrf1 mutants express high levels of genes controlling S-phase and have many more cells undergoing DNA replication...

.... This observation suggested that the maintenance error of CpG methylation on LINE-1 during DNA replication often occurs in the cultivation of wild-type ESCs, which was consistent with a recent study ( Arand et al., 2012 ). The frequency of hemimethylated LINE-1 CpG sites was significantly increased in Uhrf1...

...Kristopher H. McConnell; Michael Dixon; Brian R. Calvi DNA replication origin activity changes during development. Chromatin modifications are known to influence the genomic location of origins and the time during S phase that they initiate replication in different cells. However, how chromatin...

... destruction of Cdt1 limits DNA replication to a single round per cell cycle in Xenopus egg extracts . Genes Dev. 19 , 114 – 126 . Arias E. E. , Walter J. C. ( 2006 ). PCNA functions as a molecular platform to trigger Cdt1 destruction and prevent re-replication . Nat. Cell Biol. 8...

... progression during C. elegans development, both in the germ line and in the early embryo. Our results indicate that LRR-1 acts as a nuclear substrate-recognition subunit of a Cullin 2-RING E3 ligase complex (CRL2 LRR-1 ), which ensures DNA replication integrity. LRR-1 contains a typical BC/Cul-2 box and binds...

... College London, Gower Street, London WC1E 6BT, UK 26 11 2004 ©2005. 2005 Cell cycle GFP-PCNA XCL100 ERK Sea urchin DNA replication MAP kinase signalling pathways play major roles in cell proliferation(reviewed by Hulleman and Boonstra,2001 ; Roovers and Assoian,2000...

... these developmentally specific types of DNA replication. Moreover, the ability of the CDK site mutant to rapidly inhibit BrdU incorporation suggests that Dup is required for fork elongation during amplification. In the context of findings from human and other cells, our results indicate that stringent regulation of Dup...

... for the activity of nearby DNA replication origin(s). Ori-β is the major origin in the locus, and is a sequence-specific element that is sufficient for high-level amplification in combination with ACE3. Sequence requirements for amplification were examined using a transgenic construct that was buffered from...

... ). Embryos lacking Gnu protein undergo DNA replication and centrosome proliferation without chromosome condensation or mitotic segregation. We have identified the gnu gene encoding a novel phosphoprotein dephosphorylated by Protein phosphatase 1 at egg activation. Gnu is normally expressed in the nurse cells...

...Michelle F. Pflumm; Michael R. Botchan ABSTRACT The origin recognition complex (ORC) is a six subunit complex required for eukaryotic DNA replication initiation and for silencing of the heterochromatic mating type loci in Saccharomyces cerevisiae . Our discovery of the Drosophila ORC complex...

...Douglas D. Fenger; Janet L. Carminati; Deborah L. Burney-Sigman; Helena Kashevsky; Jessica L. Dines; Lisa K. Elfring; Terry L. Orr-Weaver ABSTRACT Following completion of meiosis, DNA replication must be repressed until fertilization. In Drosophila , this replication block requires the products...

.... Amplification proceeds through repeated firing of one or more DNA replication origins located near the center of each gene cluster. Hypomorphic mutant alleles of the chiffon gene cause thin, fragile chorions and female sterility, and were found to eliminate chorion gene amplification. Null alleles of chiffon...

... on the first DNA replication. Moreover, activation of microinjected transgenes from proximal enhancers occurs in the late 2-cell embryo and in the male pronucleus of 1-cell embryos blocked for DNA replication; whereas, for integrated transgenes, proximal enhancer activity is subject to position effects...

..., sealed with nail varnish and examined under a Zeiss photomicroscope III. In a previous report ( Hutchison et al . 1987 ), we had demonstrated that extracts prepared from Xenopus eggs supported periodic DNA replication on sperm pronuclei. In addition, the periodic properties of the cytoplasm...

... be integrated into the fish genome, that the resulting transgenic fish are mosaic and that some of these mosaic individuals give rise to stable lines of transgenic fish. 23 02 1988 © 1988 by Company of Biologists 1988 germ-line transgenic zebrafish microinjection mosaicism DNA...

...Susannah Varmuza; Valerie Prideaux; Rashmi Kothary; Janet Rossant Mouse trophoblast giant cells undergo successive rounds of DNA replication resulting in amplification of the genome. It has been difficult to determine whether giant cell chromosomes are polyploid as in liver cells or polytene...

... of the polypeptide by developing muscle cells. Embryos that had been permanently arrested with aphidicolin in the early cleavage stages up to the 32-cell stage did not express the antigen. DNA replications may be required for the antigen expression. Embryos that had been arrested with cytochalasin B in the 8-cell...

... of these activities is considered and their relationship in integrating DNA synthesis during early cleavage is discussed. Copyright © 1985 by Company of Biologists 1985 oocyte maturation cell cycle DNA replication mitosis maturation promoting factor (MPF) cytostatic factor (CSF) meiosis amphibia...