... of the distinct molecular mechanisms modulating BAF chromatin binding, including its combinatory assemblies, DNA/histone modification-binding modules and post-translational modifications, as well as its interactions with proteins, RNA and lipids. This Review aims to serve as a primer for future studies to decode...

...Mark D. Biggin; William McGinnis ABSTRACT Recent advances have shed new light on how the Q50 homeoproteins act in Drosophila . These transcription factors have remarkably similar and promiscuous DNA-binding specificities in vitro; yet they each specify distinct developmental fates in vivo. One...

... shares many features with X-inactivation, including differential DNA methylation. In this paper we consider when methylation differences in early development affecting X-chromosome activity and imprinting are established. There are processes of methylation and demethylation occurring in early development...

... of human globin mRNA and protein when this DNA was injected into fertilized eggs. Further localization of regulatory sequences was accomplished by injecting marked, 5’-deleted keratin gene DNA into fertilized eggs and evaluating tissue specificity of expression. All 5’ flanking DNA upstream from -487 could...

... studies have been carried out by gene transfer, and are supported by in vitro analyses of DNA-protein interactions in the regulatory regions of two genes that are expressed in a lineage-specific manner. Expression of both of these markers of cell fate specification is controlled by diffusible DNA-binding...

...-chromosomally localized to Xql3–q21.1, using anonymous probe DXYS1; a LOD score of 3· 07 was obtained. We are preparing cosmid libraries from DNA from mouse cell lines containing only the relevant part of the human X chromosome, introduced by chromosome-mediated gene transfer. Cosmids that contain human X...

... of cells (protoderm) which faces the blastocyst cavity. Also, all cells in female marsupial conceptuses so far examined show preferential paternal X-inactivation. To test for a possible correlation between cell position, paternal X-inactivation and DNA hypomethylation, marsupial DNA preparations from three...

...P.T. Sharpe; J. R. Miller; E. P. Evans; M. D. Burtenshaw; S. J. Gaunt1 A homeobox-containing clone has been isolated from an adult mouse kidney cDNA library and shown by DNA sequence analysis to be a new isolate, Hox-6·1 † . A genomic clone containing Hox-6 . 1 has been isolated and found...

...Roberta R. Franks; Barbara R. Hough-Evans; Roy J. Britten; Eric H. Davidson A method is described for microinjection of cloned DNA into the zygote nucleus of Lytechinus variegatus . Eggs of this species are unusually transparent, facilitating visual monitoring of the injection process. The initial...

...Jan Tesarik; Vaclav Kopečný; Michelle Plachot; Jacqueline Mandelbaum ABSTRACT Human embryos from the 2-cell to the morula stage, obtained by in vitro fertilization, were incubated with [ 3 H]thymidine or [ 3 H]uridine so as to achieve labelling of all replicating nuclear DNA and the newly...

...Lalji Singh; Shoichi Matsukuma; K. W. Jones ABSTRACT A study, by means of Y-chromosome-specific repeated DNA probes, of mouse (ST) with small testes is reviewed. Mouse ST was shown to be a somatic mosaic of 10 % XY and 90 % XX cells. The cellular composition of the azoospermie testis reflected...

... layers. It is proposed that much of the methylation observed in somatic tissues acts to stabilize and reinforce prior events that regulate the activity of specific genes, chromosome domains or the X chromosome (in females). Fetal germ cell DNA is markedly undermethylated and we favour the idea...

... close correlation of the observed structural changes with the cell cycle data ( Newport & Kirschner, 1982 ; for recent review see Satoh, 1985 ). For structural studies on embryo-injected DNA we chose injection experiments of bacteriophage λ-DNA for the following reasons. (i...