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Keywords: Cell tracking
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Journal Articles
Journal: Development
Development (2022) 149 (21): dev200292.
Published: 31 October 2022
...-GFP/EnVT15159-MS2 injected with either buffer (BI) or dsRNA against ftz ( ftz KD). Cell tracking based on the Gap43-mCherry membrane signal, spatiotemporal movie synchronisation, domain strain rate calculations, cell selection criteria and contoured heat map generation were performed...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (18): dev198994.
Published: 9 September 2021
... and applications to lineage tracing in live-imaging data and outline software methodologies that can be applied to various cell-tracking challenges. Beyond the introduction and advancement of new microscopes capable of imaging large and sensitive specimens (of which there has been an explosion in recent years...
Journal Articles
Journal: Development
Development (2021) 148 (18): dev199822.
Published: 1 September 2021
.... Nanobody Nucleus GFP maturation Live in vivo imaging Cell tracking Drosophila melanogaster National Institutes of Health http://dx.doi.org/10.13039/100000002 R35GM118146 American Heart Association http://dx.doi.org/10.13039/100000968 18POST34080493 California...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (9): 1725–1734.
Published: 1 May 2017
... are necessary to systematically analyze epithelial organization and dynamics at single-cell resolution. We developed SEGGA, an easy-to-use software for automated image segmentation, cell tracking and quantitative analysis of cell shape, polarity and behavior in epithelial tissues. SEGGA is free, open source...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (14): 2895–2900.
Published: 15 July 2014
... evaluated this functionality of EDGE4D based on the neighbor relations of cells. Since cells undergo minimal neighbor exchange and do not divide during dorsal fold formation, EDGE4D should report stable cell-cell contacts if the cell tracking is accurate ( Fig. 1 H). To evaluate neighbor stability, we...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2013) 140 (15): 3266–3274.
Published: 1 August 2013
... Cell tracking Caenorhabditis elegans The interpretation of complex in vivo phenotypes is a crucial and challenging step required to achieve a functional understanding of molecular networks. Advances in imaging technology offer an unprecedented opportunity for systems-level studies...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2013) 140 (2): 459–470.
Published: 15 January 2013
... this intriguing concept, we developed a single-cell tracking approach that enables automatic detection of cell cycle phases in live (stem) cells expressing fluorescent ubiquitylation-based cell-cycle indicator (FUCCI) probes. Using this tool, we have identified distinctive changes in lengths and fluorescence...
Includes: Multimedia, Supplementary data
Journal Articles
Journal: Development
Development (2012) 139 (2): 359–372.
Published: 15 January 2012
... supported model. Here, we combine four-dimensional imaging with custom cell tracking software and photoactivatable fluorophore labeling to determine the cellular dynamics underlying OCM in zebrafish. Although cell division contributes to growth, we find it dispensable for eye formation. OCM depends instead...
Includes: Multimedia, Supplementary data