... mass compaction. We show that nebel mutants exhibit reduced furrow-associated slow calcium waves (SCWs), caused at least in part by defective enrichment of calcium stores. RNP and FMA distal enrichment mirrors the medial-to-distal polarity of SCWs, and inhibition of calcium release or downstream...

... to fertilization. RGS2 G q Oocyte Meiotic maturation Calcium Egg activation Fully grown mammalian oocytes are arrested in meiotic prophase until a mid-cycle release of luteinizing hormone from the pituitary stimulates resumption of meiosis to the metaphase II (MII) stage, referred to herein...

...Isabela Ramos; Adrian Reich; Gary M. Wessel Egg activation at fertilization is an excellent process for studying calcium regulation. Nicotinic acid adenine dinucleotide-phosphate (NAADP), a potent calcium messenger, is able to trigger calcium release, likely through two-pore channels (TPCs...

...Shih-Lei Lai; Wan-Ling Yao; Ku-Chi Tsao; Anna J. S. Houben; Harald M. H. G. Albers; Huib Ovaa; Wouter H. Moolenaar; Shyh-Jye Lee Left-right (L-R) patterning is essential for proper organ morphogenesis and function. Calcium fluxes in dorsal forerunner cells (DFCs) are known to regulate the formation...

...Ludmila Francescatto; Sarah C. Rothschild; Alexandra L. Myers; Robert M. Tombes Intracellular calcium ion (Ca 2+ ) elevation on the left side of the mouse embryonic node or zebrafish Kupffer's vesicle (KV) is the earliest asymmetric molecular event that is functionally linked to lateral organ...

.... * Author for correspondence ( keith.jones@newcastle.edu.au ) 30 9 2009 © 2009. 2009 Calcium Cell cycle Fertilization Mouse CamKII (Camk2) Mouse eggs are ovulated following arrest at metaphase of the second meiotic division (metII). Fertilization breaks this arrest...

... is synergistic with that of progesterone and that this synergism is potentially highly significant in gamete interactions leading to fertilisation. * Author for correspondence (e-mail: s.j.publicover@bham.ac.uk ) 22 9 2008 © 2008. 2008 Calcium Cumulus Motility Nitric oxide Oviduct...

... a conserved early Ca 2+ requirement in DFC-like cell function in zebrafish and Xenopus . * Author for correspondence (e-mail: diane-slusarski@uiowa.edu ) 10 10 2007 © 2008. 2008 Calcium Laterality β-catenin Zebrafish Xenopus Dorsal forerunner cells Although vertebrates...

... of trunk muscles. Electrophysiological recordings from muscle showed that output from the CNS was normal in mutants, suggesting a defect in the muscle. Calcium imaging revealed that Ca 2+ transients were reduced in mutant fast muscle. Immunostaining demonstrated that ryanodine and dihydropyridine receptors...

...). * Author for correspondence (e-mail: kuwada@umich.edu ) 16 8 2004 © 2004. 2004 Zebrafish Accordion Behavior Muscle Calcium SERCA1 Brody diseasea Several factors have made the zebrafish an attractive vertebrate model for the study of motor development. First, forward...

... the micropipet from sticking to the chorion and follicle cells. Each micropipet was used only once. Protein and IP 3 concentrations given in the text refer to the final concentration in the egg cytoplasm. Ca 2+ measurements were made using calcium green-1 10-kDa dextran (Molecular Probes, Eugene, OR, USA...

...Mark Levasseur; Alex McDougall ABSTRACT The generation of calcium oscillations at fertilisation and during mitosis appears to be controlled by the cell cycle machinery. For example, the calcium oscillations in oocytes and embryos occur during metaphase and terminate upon entry into interphase. Here...

... . Dev. Biol . 116 , 519 – 523 . 10.1016/0012-1606(86)90152-1 Arnoult , C. , Lemos , J. R. , and Florman , HM . ( 1997 ). Voltage-dependent modulation of T-type calcium channels by protein tyrosine phosphorylation . EMBO J . 16 , 1593 – 1599 . 10.1093/emboj/16.7.1593...

... to define more precisely the function of this protein in the regulation of actin-bundle assembly in nurse cells. We report that recombinant Quail can bind and bundle filamentous actin in vitro in a manner similar to villin at a physiological calcium concentration. In contrast to villin, Quail is unable...

...Joanne Shearer; Céline De Nadai; Françoise Emily-Fenouil; Christian Gache; Michael Whitaker; Brigitte Ciapa ABSTRACT It is well known that stimulation of egg metabolism after fertilization is due to a rise in intracellular free calcium concentration. In sea urchin eggs, this first calcium signal...

... fertilisation. Sperm trigger a series of Ca 2+ oscillations that pause for 5 minutes while only the MPF activity is low and are present when both MPF and MAP kinase activity are elevated. We next attempted to determine whether the second phase of calcium oscillations is required to reactivate the MPF activity...

... 1998 Blastocyst Implantation Calcitonin receptor Calcium Integrin Mouse Establishment of pregnancy in mammals is dependent upon successful completion of the embryonic program leading to implantation of the blastocyst in the uterine wall. Bioactive agents produced within the maternal...

... absence of signal ( Fig. 2D ), confirming the specificity of the immunostaining. Furthermore, after artificial induction of the acrosome reaction with calcium ionophores in epididymal spermatozoa, no decrease in the amount of the 24 kDa tr-kit protein was detected by western blot analysis with respect...

... calcium fertilization sperm-egg fusion mouse The fertilizing sperm activates all vertebrate and invertebrate eggs by generating [Ca 2+ ] i transients in the egg cytoplasm ( Jaffe, 1983 , Whitaker and Steinhardt, 1982 ; Nuccitelli, 1991 ; Swann and Ozil, 1994 ). In nearly all cases the [Ca 2...

... for the Promotion of Science for Young Scientists to R. D. (No. 4141) and Grants-in-Aid from the Japanese Ministry of Education, Science, Culture and Sports to M. M. (No. 06559005). REFERENCES Berridge , M. J. ( 1993a ). Inositol trisphosphate and calcium signalling . Nature 361 , 315 – 325...