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Keywords: CRISPR/Cas9
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Journal Articles
Journal: Development
Development (2022) 149 (17): dev200552.
Published: 8 September 2022
... . 10.18637/jss.v031.i10 Bhattacharya , D. , Marfo , C. A. , Li , D. , Lane , M. and Khokha , M. K. ( 2015 ). CRISPR/Cas9: An inexpensive, efficient loss of function tool to screen human disease genes in Xenopus . Dev. Biol. 408 , 196 - 204 . 10.1016/j.ydbio.2015.11.003...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2022) 149 (11): dev200644.
Published: 6 June 2022
... currently used sea urchin. We leveraged this advantage to generate a knockout mutant of the sea urchin homolog of the drug transporter ABCB1, a major player in xenobiotic disposition for all animals. Using CRISPR/Cas9, we generated large fragment deletions of ABCB1 and used these readily detected deletions...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (24): dev199866.
Published: 23 December 2021
... of the motor domain acts as a STOP. In contrast, uncoordinated mitochondrial motility was observed in CRISPR/Cas9-GSK3β non-phosphorylatable-KHCS314A Drosophila larval axons, owing to decreased kinesin 1 attachment to microtubules and/or membranes, and reduced ATPase activity. Together, we propose that GSK3β...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (20): dev194613.
Published: 22 October 2021
... that MESP1 and MESP2 are required for mesoderm specification in a dose-dependent manner. Mesoderm specification MESP Canonical Wnt signaling CRISPR/Cas9 Japan Society for the Promotion of Science http://dx.doi.org/10.13039/501100001691 The mesodermal cells are derived from...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2021) 148 (5): dev196899.
Published: 9 March 2021
... in the mouse inner ear and also in other organs. Summary: We have established a quick genetic method, CRISPR-stop, that reveals the functions of lethal genes without the need to generate conditional knockout mice and breeding. CRISPR/Cas9 Base editing Rbm24 Atoh1 Hair cells Inner ear...
Includes: Supplementary data
Journal Articles
In collection:
Plant development
Journal: Development
Development (2021) 148 (5): dev194894.
Published: 9 March 2021
... of Biologists Ltd 2021 Summary: An APETALA2-like transcription factor has profound roles in pre-fertilisation and post-fertilisation reproductive development in barley. Barley AP2 Floret Grain CRISPR/Cas9 MADS-box BOP1/2 B-sister Nucellus Integument Sex in angiosperms occurs...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (9): dev175976.
Published: 14 May 2019
...Masanori Abe; Reiko Kuroda ABSTRACT The establishment of left-right body asymmetry is a key biological process that is tightly regulated genetically. In the first application of CRISPR/Cas9 to a mollusc, we show decisively that the actin-related diaphanous gene Lsdia1 is the single maternal gene...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2019) 146 (8): dev172866.
Published: 23 April 2019
... to clarify their roles in sex reversal, biallelic mutants for crhr1 and crhr2 were produced by CRISPR/Cas9 technology. Remarkably, biallelic mutants of both loci ( crhr1 and crhr2 ) did not undergo female-to-male sex reversal upon exposure to HT. Inhibition of this process in double corticotropin-releasing...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2018) 145 (20): dev168906.
Published: 17 October 2018
... interaction and the compensatory mechanisms involved can make findings from single mutations, at best difficult to interpret, and, at worst, misleading. Hence, it is necessary to establish an efficient way to disrupt multiple genes simultaneously. CRISPR/Cas9-mediated base editing disrupts gene function...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (15): 2852–2858.
Published: 1 August 2017
...-directed repair (HDR) that provides efficient non-mosaic targeted insertion of small DNA fragments (40-50 nucleotides) in 4.4-25.7% of F0 tadpoles, with germline transmission. For both CRISPR/Cas9-mediated HDR gene editing and indel mutation, the gene-edited F0 embryos are uniformly heterozygous...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (6): 1025–1034.
Published: 15 March 2017
.../ Summary: In human cerebral organoids, depletion of the tumor suppressor retinoblastoma protein disrupts proliferation, promotes entry into S-phase, and causes increased apoptosis and aberrant neuronal migration. Retinoblastoma Human embryonic stem cells Organoid Brain CRISPR/Cas9 Cell cycle...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2017) 144 (4): 635–648.
Published: 15 February 2017
... compatible with targeted genetic manipulations. However, gene targeting, which is crucial for functional studies of embryonic stem cells, has not been exploited to date in NSC lines. Here, we deploy CRISPR/Cas9 technology to demonstrate a variety of sophisticated genetic modifications via gene targeting...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (23): 4405–4418.
Published: 1 December 2016
... these limitations by developing single-step optimized inducible gene knockdown or knockout (sOPTiKD or sOPTiKO) platforms. These are based on genetic engineering of human genomic safe harbors combined with an improved tetracycline-inducible system and CRISPR/Cas9 technology. We exemplify the efficacy...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2016) 143 (15): 2868–2875.
Published: 1 August 2016
...Ira L. Blitz; Margaret B. Fish; Ken W. Y. Cho CRISPR/Cas9 genome editing is revolutionizing genetic loss-of-function analysis but technical limitations remain that slow progress when creating mutant lines. First, in conventional genetic breeding schemes, mosaic founder animals carrying mutant...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2015) 142 (20): 3601–3611.
Published: 15 October 2015
...Fuyi Chen; Joel Rosiene; Alicia Che; Albert Becker; Joseph LoTurco The ability to induce targeted mutations in somatic cells in developing organisms and then track the fates of those cells is a powerful approach both for studying neural development and for modeling human disease. The CRISPR/Cas9...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2014) 141 (21): 4115–4120.
Published: 1 November 2014
...Alberto Stolfi; Shashank Gandhi; Farhana Salek; Lionel Christiaen The CRISPR/Cas9 system has ushered in a new era of targeted genetic manipulations. Here, we report the use of CRISPR/Cas9 to induce double-stranded breaks in the genome of the sea squirt Ciona intestinalis. We use electroporation...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2013) 140 (24): 4982–4987.
Published: 15 December 2013
...Alexander Hruscha; Peter Krawitz; Alexandra Rechenberg; Verena Heinrich; Jochen Hecht; Christian Haass; Bettina Schmid Gene modifications in animal models have been greatly facilitated through the application of targeted genome editing tools. The prokaryotic CRISPR/Cas9 type II genome editing...
Includes: Supplementary data