... the morphology of the adult organs. In the ovary, Chsy mutants exhibited altered stiffness of the basement membrane and muscle dysfunction, leading to a gradual degradation of the gross organ structure as mutant animals aged. Our observations show that normal CS function is required for the maintenance...

...Daniel S. Costa; Isabel W. Kenny-Ganzert; Qiuyi Chi; Kieop Park; Laura C. Kelley; Aastha Garde; David Q. Matus; Junhyun Park; Shaul Yogev; Bob Goldstein; Theresa V. Gibney; Ariel M. Pani; David R. Sherwood ABSTRACT Cell invasion through basement membrane (BM) barriers is important in development...

... conditional null mutants showed defective cell-cell and cell-matrix adhesion, both of which have been shown to play instructive roles in salivary gland branching. Loss of FGF signaling led to disordered cell-basement membrane interactions in vivo as well as in organ culture. This was partially restored upon...

... is required for the assembly but not the maintenance of the basement membrane. Lastly, we showed that further deletion of C4st1, a chondroitin sulfate (CS) sulfation enzyme, did not affect the assembly of the ILM but, when combined with Ext1 deletion, it aggravated the retinal permeability by disrupting...

...Uwe Töpfer; Karla Yanín Guerra Santillán; Elisabeth Fischer-Friedrich; Christian Dahmann ABSTRACT The basement membrane is a specialized extracellular matrix (ECM) that is crucial for the development of epithelial tissues and organs. In Drosophila , the mechanical properties of the basement...

...Johanna I. Englund; Alexandra Ritchie; Leander Blaas; Hanne Cojoc; Nalle Pentinmikko; Julia Döhla; Sharif Iqbal; Manuel Patarroyo; Pekka Katajisto ABSTRACT Epithelial attachment to the basement membrane (BM) is essential for mammary gland development, yet the exact roles of specific BM components...

...Véronique Van De Bor; Vincent Loreau; Marilyne Malbouyres; Delphine Cerezo; Audrey Placenti; Florence Ruggiero; Stéphane Noselli ABSTRACT Basement membranes (BM) are extracellular matrices assembled into complex and highly organized networks essential for organ morphogenesis and function. However...

... and lateral nasal processes. We demonstrate that Prickle1 , a key component of planar cell polarity signaling, is expressed in progenitors of the PTD and throughout tear duct morphogenesis. Disruption of Prickle1 stalls tear duct elongation; in particular, the loss of basement membrane deposition and aberrant...

... performed a microarray analysis that identified Fgfbp1 as a novel Wnt/β-catenin-regulated gene in mouse brain endothelial cells (mBECs). Fgfbp1 is expressed in the CNS endothelium and secreted into the vascular basement membrane during BBB formation. Endothelial genetic ablation of Fgfbp1 results...

... Wallingford ABSTRACT Laminin alpha 5 (LAMA5) is a member of a large family of proteins that trimerise and then polymerise to form a central component of all basement membranes. Consequently, the protein plays an instrumental role in shaping the normal development of the kidney, skin, neural tube, lung...

... differences between these two CNS regions with regard to how neurovascular barrier properties arise and are maintained. Table 2. Expression of major basement membrane receptors in the CNS neurovascular unit Table 1. Cellular sources of major vascular basement membrane molecules...

... been proposed that after Fat2-dependent planar polarization of the follicle cell basal domain, oriented basement membrane (BM) fibrils and F-actin stress fibers constrain follicle growth, promoting its axial elongation. However, the relationship between BM fibrils and stress fibers and their respective...

... in the developing optic cup require neural crest. Ultrastructural analysis reveals that basement membrane formation around the developing eye is also dependent on neural crest, but only specifically around the retinal pigment epithelium. Neural crest cells produce the extracellular matrix protein nidogen: impairing...

...-dependent synthesis of laminin alpha1 controls basement membrane assembly in the myotome . Development   136 , 3495 - 3504 . 10.1242/dev.036087 Bartel , D. P. ( 2009 ). MicroRNAs: target recognition and regulatory functions . Cell   136 , 215 - 233 . 10.1016/j.cell.2009.01.002...

...Julien Chlasta; Pascale Milani; Gaël Runel; Jean-Luc Duteyrat; Leticia Arias; Laurie-Anne Lamiré; Arezki Boudaoud; Muriel Grammont The regulation of morphogenesis by the basement membrane (BM) may rely on changes in its mechanical properties. To test this, we developed an atomic force microscopy...

... depends on epidermal cellular protrusions that directly contact pObs only where an otherwise occluding basement membrane remains incompletely assembled. Progressively separated pObs pools then continue regenerating independently to collectively re-form a now branched skeletal structure. * Authors...

... development, yet the underlying mechanism has remained elusive. We show that a unique composition of blood vessels facilitates the role of the endothelium in bone mineralization and morphogenesis. Immunostaining and electron microscopy showed that the endothelium in developing bones lacks basement membrane...

...William P. Daley; Elise M. Gervais; Samuel W. Centanni; Kathryn M. Gulfo; Deirdre A. Nelson; Melinda Larsen The basement membrane is crucial for epithelial tissue organization and function. However, the mechanisms by which basement membrane is restricted to the basal periphery of epithelial tissues...

... 12.5 kidneys revealed an almost complete absence of basement membrane proteins and reduced levels of α6 integrin and FGF2. mRNA levels for fibroblast growth factor 2 (FGF2) and mediators of the GDNF/RET and WNT11 signaling pathway were also decreased. Furthermore, collecting duct cells derived from...

... in the fully delaminated epicardium. The present gene-targeting experiments demonstrate that mouse embryos lacking Flrt2 expression arrest at mid-gestation owing to cardiac insufficiency. The defects in integrity of the epicardial sheet and disturbed organization of the underlying basement membrane closely...