IN THIS ISSUE
Summary: This Spotlight article briefly describes how several different religions perceive the human embryo, and considers how this might influence their view of human embryological research.
Summary: This Spotlight discusses the potential of interspecies chimeras for human stem cell research but also the significant barriers and ethical issues surrounding their generation.
Summary: This Review discusses how different classes of miRNAs fit into the hierarchy of gene regulatory networks that control development, from early embryogenesis to cell differentiation.
STEM CELLS AND REGENERATION
BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice
Summary: A Gli1 transgenic mouse model reveals that BMP signaling regulates mesenchymal stem cell fate during molar root development.
Synergism between canoe and scribble mutations causes tumor-like overgrowth via Ras activation in neural stem cells and epithelia
Summary: In Drosophila, simultaneous loss of Cno and Scrib results in disrupted asymmetric cell division and Ras-PI3K-Akt activation in neuroblasts, and in ectopic Ras-MAPK signaling in epithelia.
Yorkie and Hedgehog independently restrict BMP production in escort cells to permit germline differentiation in the Drosophila ovary
Summary: Integrated and independent activities of the Yki and Hh signaling pathways guide the maintenance, differentiation and proliferation of somatic and germline stem cells of the Drosophila germarium.
Three-dimensional structural analysis reveals a Cdk5-mediated kinase cascade regulating hepatic biliary network branching in zebrafish
Highlighted article: Inactivation of Cdk5, or its regulator Cdk5r1a, leads to reduced network branching and disrupts actin organization in the zebrafish adult intrahepatic biliary network.
Setd1b, encoding a histone 3 lysine 4 methyltransferase, is a maternal effect gene required for the oogenic gene expression program
Highlighted article: Oocyte-specific knockout of Setd1b in mouse disrupts the zona pellucida, metabolic processes and zygote morphology, identifying Setd1b as a key regulator of oocyte development.
Highlighted article: Loss of Sin3a in the mouse early foregut endoderm leads to defects in lung development and cell cycle arrest of epithelial progenitor cells.
SoxF factors induce Notch1 expression via direct transcriptional regulation during early arterial development
Summary: Identification of novel enhancers in vertebrate Notch1 genes places SoxF transcription factors directly upstream of Notch signalling in controlling arterial specification and differentiation.
Antagonistic regulation of the second mitotic wave by Eyes absent-Sine oculis and Combgap coordinates proliferation and specification in the Drosophila retina
Summary: Combgap acts as a novel negative regulator of Eya-So in transcriptional outputs that coordinate cell cycle exit with the transition to differentiation during Drosophila retinal development.
Summary: Development of dHb neurons and posteriorly directed outgrowth of their axons are regulated by Shh, Wnt, Fgf, and chemokine signaling pathways in zebrafish.
Architectural protein Pita cooperates with dCTCF in organization of functional boundaries in Bithorax complex
Summary: The chromatin architectural protein Pita functions much like dCTCF in the organization of Drosophila BX-C, providing insight into how boundaries impact BX-C gene regulation and segment specification.
COP9 signalosome subunits protect Capicua from MAPK-dependent and -independent mechanisms of degradation
Summary: Mutations in the COP9 signalosome component CSN1b promote degradation of the tumor suppressor Capicua, by both a cullin-mediated EGFR-dependent mechanism and by an alternative route.
SPEF2 functions in microtubule-mediated transport in elongating spermatids to ensure proper male germ cell differentiation
Summary: The cilia-related protein SPEF2 is involved in intracellular transport during sperm tail formation via an interaction with cytoplasmic dynein 1, IFT20 and GOLGA3 proteins.
TECHNIQUES AND RESOURCES
Summary: A simple method to target GFP-tagged proteins for proteasomal degradation enables spatially controlled protein elimination in C. elegans.