Stomata are epidermal pores that control gas exchange between plants and the atmosphere. In Arabidopsis, the ERECTA family (ERECTAf), including ERECTA, ERECTA-LIKE1 (ERL1), and ERL2, redundantly play pivotal roles in enforcing the so-called “one-cell-spacing” rule. Accumulating evidences demonstrated that the functional specificities of receptors are likely associated with their differential subcellular dynamics. The endoplasmic reticulum (ER)-resident chaperone complex SDF2-ERdj3B-BiP functions in many aspects of plant development. We employed pharmacological treatments combined with cell biological and biochemical approaches to demonstrate that the abundance of ERECTA was reduced in erdj3b-1 mutant, but the localization, and dynamics of ERECTA were barely affected. By contrast, the erdj3b mutation caused the retention of ERL1/ERL2 in the ER. Furthermore, we found that the function of SDF2-ERdj3B-BiP is implicated with the distinct roles of ERECTAf receptors. Our findings establish that the ERECTAf receptor-mediated signaling in stomatal development is ensured by the activities of ER quality control system (ERQC) that preferentially maintain protein abundance of ERECTA and proper subcellular dynamics of ERL1/ERL2, respectively, prior to the receptors reaching their destination, the plasma membrane, to execute their functions.
The functional specificity of ERECTA-family receptors in Arabidopsis stomatal development is ensured by molecular chaperones in the endoplasmic reticulum
These authors contributed equally to the work
- Award Group:
- Funder(s): National Natural Science Foundation of China
- Award Id(s): 31871377
- Funder(s):
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Ke-Zhen Yang, Chao-Ran Zuo, Ya-Jun Leng, Jun-Ling Yue, Hui-Chao Liu, Zhi-Bin Fan, Xue-Yi Xue, Juan Dong, Li-Qun Chen, Jie Le; The functional specificity of ERECTA-family receptors in Arabidopsis stomatal development is ensured by molecular chaperones in the endoplasmic reticulum. Development 2022; dev.200892. doi: https://doi.org/10.1242/dev.200892
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