Despite the growing interest in the rabbit model for developmental and stem cell biology, the characterization of embryos at the molecular level is still poorly documented. We conducted a transcriptome analysis of rabbit pre-implantation embryos from E2.7 (morula stage) to E6.6 (early primitive streak stage) using bulk and single-cell RNA-sequencing. In parallel, we studied oxidative phosphorylation and glycolysis and analysed active and repressive epigenetic modifications during blastocyst formation and expansion. We generated a transcriptomic, epigenetic, and metabolic map of the pluripotency continuum in rabbit preimplantation embryos and identified novel markers of naive pluripotency that might be instrumental for deriving naive pluripotent stem cell lines. Although the rabbit is evolutionarily closer to mice than to primates, we found that the transcriptome of rabbit epiblast cells shares common features with that of humans and non-human primates.
Major transcriptomic, epigenetic and metabolic changes underly the pluripotency continuum in rabbit preimplantation embryos
- Award Group:
- Funder(s): Agence Nationale de la Recherche
- Award Id(s): ANR-18-CE13-023 Oryctocell
- Funder(s):
- Award Group:
- Funder(s): Fondation pour la Recherche Medicale
- Award Id(s): DEQ20170336757
- Funder(s):
- Award Group:
- Funder(s): Agence Nationale de la Recherche
- Award Id(s): ANR-11-LABX-0042
- Funder(s):
- Award Group:
- Funder(s): Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
- Funder(s):
Currently Viewing Accepted Manuscript - Newer Version Available
Wilhelm Bouchereau, Luc Jouneau, Catherine Archilla, Irène Aksoy, Anais Moulin, Nathalie Daniel, Nathalie Peynot, Sophie Calderari, Thierry Joly, Murielle Godet, Yan Jaszczyszyn, Marine Pratlong, Dany Severac, Pierre Savatier, Véronique Duranthon, Marielle Afanassieff, Nathalie Beaujean; Major transcriptomic, epigenetic and metabolic changes underly the pluripotency continuum in rabbit preimplantation embryos. Development 2022; dev.200538. doi: https://doi.org/10.1242/dev.200538
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