The mitochondrial matrix AAA+ Lon protease (LONP1) degrades misfolded or unassembled proteins, which play a pivotal role in mitochondrial quality control. During heart development, a metabolic shift from anaerobic glycolysis to mitochondrial oxidative phosphorylation takes place, and this process relies highly on functional mitochondria. However, the relationship between mitochondrial quality control machinery and metabolic shifts is elusive. Here, we interfered with mitochondrial quality control by inactivating Lonp1 in embryonic cardiac tissue and found severely impaired heart development, leading to embryonic lethality. Mitochondrial swelling, cristae loss and abnormal protein aggregates were evident in the mitochondria of Lonp1-deficient cardiomyocytes. Accordingly, the p-eIF2α-ATF4 pathway was triggered, and nuclear translocation of ATF4 was observed. We further demonstrated that ATF4 negatively regulates the expression of Tfam while promoting that of Glut1, which was responsible for the disruption of the metabolic shift to oxidative phosphorylation. Meanwhile, elevated levels of reactive oxygen species were observed in Lonp1 mutant cardiomyocytes. This study revealed that LONP1 safeguards metabolic shifts in the developing heart by controlling mitochondrial protein quality and implies that disrupted mitochondrial quality control may cause prenatal cardiomyopathy.
LONP1-mediated mitochondrial quality control safeguards metabolic shifts in heart development
- Award Group:
- Funder(s): National Key Research and Development Program of China
- Award Id(s): 2019YFA0801601
- Funder(s):
Currently Viewing Accepted Manuscript - Newer Version Available
Ke Zhao, Xinyi Huang, Wukui Zhao, Bin Lu, Zhongzhou Yang; LONP1-mediated mitochondrial quality control safeguards metabolic shifts in heart development. Development 2022; dev.200458. doi: https://doi.org/10.1242/dev.200458
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