Rice (Oryza sativa) is one of our main food crops, feeding around 3.5 billion people worldwide. An increasing number of studies note the importance of the cytoskeleton, including actin filaments and microtubules, on rice development and environmental responses. Yet, reliable in vivo cytoskeleton markers are lacking in rice, which critically limits our knowledge of cytoskeletal functions in living cells. Therefore, we generated bright fluorescent marker lines of the actin and microtubule cytoskeletons in rice, suitable for live cell imaging in a wide variety of rice tissues. Using these lines, we show that actin bundles and microtubules engage and co-function during pollen grain development, how the cytoskeletal components are coordinated during root cell development and that the actin cytoskeleton is robust and facilitates microtubule responses during salt stress. Hence, we conclude that our cytoskeletal marker lines, highlighted by our exciting findings of cytoskeletal associations and dynamics, will substantially further future investigations in rice biology.
Fluorescent cytoskeletal markers reveal associations between the actin and microtubule cytoskeleton in rice cells
- Award Group:
- Funder(s): National Natural Science Foundation of China
- Award Id(s): 31970803
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- Award Group:
- Funder(s): Shanghai Jiao Tong University
- Award Id(s): 18X100040056
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- Award Group:
- Funder(s): China Postdoctoral Science Foundation
- Award Id(s): 2017M621451
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- Award Group:
- Funder(s): Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center)
- Award Id(s): B14016
- Funder(s):
- Award Group:
- Funder(s): Australian Research Council
- Award Id(s): DP190101941
- Funder(s):
- Award Group:
- Funder(s): VILLUM Center for Bioanalytical Sciences
- Award Id(s): 25915
- Funder(s):
- Award Group:
- Funder(s): Novo Nordisk Fonden
- Award Id(s): 19OC0056076
- Funder(s):
- Award Group:
- Funder(s): Danmarks Grundforskningsfond
- Award Id(s): DNRF155
- Funder(s):
- Award Group:
- Funder(s): Shanghai Jiao Tong University
- Award Id(s): MDS-JF-2019A05
- Funder(s):
- Award Group:
- Funder(s): National Natural Science Foundation of China
- Award Id(s): M-0141
- Funder(s):
Currently Viewing Accepted Manuscript - Newer Version Available
Zengyu Liu, Isabella Østerlund, Felix Ruhnow, Yiran Cao, Guoqiang Huang, Wenguo Cai, Jiao Zhang, Wanqi Liang, Zoran Nikoloski, Staffan Persson, Dabing Zhang; Fluorescent cytoskeletal markers reveal associations between the actin and microtubule cytoskeleton in rice cells. Development 2022; dev.200415. doi: https://doi.org/10.1242/dev.200415
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