Recent evidence has shown that presenilin enhancer2 (Pen-2) plays an essential role in corticogenesis by regulating the switch of apical progenitors (APs) to basal progenitors (BPs). The hippocampus is a brain structure required for advanced functions including spatial navigation, learning and memory. However, it remains unknown whether Pen-2 is important for hippocampal morphogenesis. To address this question, we generated Pen-2 conditional knockout (cKO) mice, in which Pen-2 is inactivated in neural progenitor cells (NPCs) in the hippocampal primordium. We showed that Pen-2 cKO mice exhibited hippocampal malformation and decreased population of NPCs in the neuroepithelium of the hippocampus. We found that deletion of Pen-2 neither affected the proliferative capability of APs nor the switch of APs to BPs in the hippocampus, and that it caused enhanced transition of APs to neurons. We demonstrated that expression of Notch1 intracellular domain (N1ICD) significantly increased the population of NPCs in the Pen-2 cKO hippocampus. Collectively, this study uncovers a critical role of Pen-2 in the maintenance of NPCs during hippocampal development.
Presenilin enhancer2 is critical for the transition of apical progenitors to neurons but not basal progenitors in the developing hippocampus
- Award Group:
- Funder(s): National Natural Science Foundation of China
- Award Id(s): 91849113 and 31271123
- Funder(s):
Currently Viewing Accepted Manuscript - Newer Version Available
Yingqian Xia, Yizhi Zhang, Min Xu, Xiaochuan Zou, Jun Gao, Mu-Huo Ji, Guiquan Chen; Presenilin enhancer2 is critical for the transition of apical progenitors to neurons but not basal progenitors in the developing hippocampus. Development 2022; dev.200272. doi: https://doi.org/10.1242/dev.200272
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