Development entails patterned emergence of diverse cell types within the embryo. In mammals, cells positioned inside the embryo give rise to the inner cell mass (ICM) that eventually forms the embryo proper. Yet the molecular basis of how these cells recognise their ‘inside’ position to instruct their fate is unknown. Here we show that provision of extracellular matrix (ECM) to isolated embryonic cells induces ICM specification and alters subsequent spatial arrangement between epiblast (EPI) and primitive endoderm (PrE) cells that emerge within the ICM. Notably, this effect is dependent on integrin β1 activity and involves apical to basal conversion of cell polarity. We demonstrate that ECM-integrin activity is sufficient for ‘inside’ positional signalling and it is required for proper EPI/PrE patterning. Our findings thus highlight the significance of ECM-integrin adhesion in enabling position-sensing by cells to achieve tissue patterning.
ECM-integrin signalling instructs cellular position-sensing to pattern the early mouse embryo
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- Funder(s): European Research Council
- Award Id(s): 742732
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- Accepted Manuscript 14 December 2021
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Esther Jeong Yoon Kim, Lydia Sorokin, Takashi Hiiragi; ECM-integrin signalling instructs cellular position-sensing to pattern the early mouse embryo. Development 2021; dev.200140. doi: https://doi.org/10.1242/dev.200140
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