Pluripotent stem cells can be classified into two distinct states, naïve and primed, which show different degrees of potency. One difficulty in stem cell research is the inability to distinguish these states in live cells. Studies on female mice have shown that reactivation of inactive X chromosomes occurs in the naïve state, while one of the X chromosomes is inactivated in the primed state. Therefore, we tried to distinguish the two states by monitoring X chromosome reactivation. Thus far, X chromosome reactivation has been analysed using fixed cells; here, we inserted different fluorescent reporter gene cassettes (mCherry and eGFP) into each X chromosome. Using these knock-in “MOMIJI” mice, we detected X chromosome reactivation accurately in live embryos, and confirmed that the pluripotent states of embryos were stable ex vivo, as represented by embryonic and epiblast stem cells in terms of X chromosome reactivation. Thus, MOMIJI mice provide a simple and accurate method for identifying stem cell status based on X chromosome reactivation.
Live imaging of X chromosome reactivation dynamics in early mouse development can discriminate naïve from primed pluripotent stem cells
Present address: KINKI University, Faculty of Biology-Oriented Science and Technology, Department of Genetic Engineering, Nishimitani 930, Kinokawa-city, Wakayama, Japan, 649-6493
Currently Viewing Accepted Manuscript - Newer Version Available
Shin Kobayashi, Yusuke Hosoi, Hirosuke Shiura, Kazuo Yamagata, Saori Takahashi, Yoshitaka Fujihara, Takashi Kohda, Masaru Okabe, Fumitoshi Ishino; Live imaging of X chromosome reactivation dynamics in early mouse development can discriminate naïve from primed pluripotent stem cells. Development 2016; dev.136739. doi: https://doi.org/10.1242/dev.136739
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