During the first phase of Arabidopsis female gamete formation (megasporogenesis), a somatic ovule cell differentiates into a megaspore mother cell and divides to generate four haploid megaspores. In the next phase (megagametogenesis), one of these megaspores undergoes syncytial mitosis and differentiates to form the female gametophyte. It’s known that a somatic small RNA (sRNA) pathway restricts reproductive potential to this functional megaspore but what controls the megasporogenesis to megagametogenesis transition? Here (p. 1399), Matthew Tucker and co-workers examine gene expression patterns in ovule tissues and show that an sRNA pathway is also involved in this phase of female gamete formation. The researchers report that ARGONAUTE5 (AGO5), a putative sRNA pathway effector, is expressed around reproductive cells during megasporogenesis and show that a unique semi-dominant ago5-4 insertion allele disrupts the initiation of megagametogenesis. Expression of a viral RNAi suppressor protein in the somatic cells flanking the megaspores produces a similar phenotype. Thus, the researchers conclude, at least two somatic sRNA pathways contribute to female gametophyte development in Arabidopsis.
sRNA paths to plant female gamete development
sRNA paths to plant female gamete development. Development 15 April 2012; 139 (8): e803. doi:
Download citation file:
Advertisement
Cited by
Call for papers: Uncovering Developmental Diversity
Development invites you to submit your latest research to our upcoming special issue: Uncovering Developmental Diversity. This issue will be coordinated by our academic Editor Cassandra Extavour (Harvard University, USA) alongside two Guest Editors: Liam Dolan (Gregor Mendel Institute of Molecular Plant Biology, Austria) and Karen Sears (University of California Los Angeles, USA).
Choose Development in 2024
In this Editorial, Development Editor-in-Chief James Briscoe and Executive Editor Katherine Brown explain how you support your community by publishing in Development and how the journal champions serious science, community connections and progressive publishing.
Journal Meeting: From Stem Cells to Human Development
Register now for the 2024 Development Journal Meeting From Stem Cells to Human Development. Early-bird registration deadline: 3 May. Abstract submission deadline: 21 June.
Pluripotency of a founding field: rebranding developmental biology
This collaborative Perspective, the result of a workshop held in 2023, proposes a set of community actions to increase the visibility of the developmental biology field. The authors make recommendations for new funding streams, frameworks for collaborations and mechanisms by which members of the community can promote themselves and their research.
Read & Publish Open Access publishing: what authors say
We have had great feedback from authors who have benefitted from our Read & Publish agreement with their institution and have been able to publish Open Access with us without paying an APC. Read what they had to say.