In early embryogenesis of spiders, the cumulus is characteristically observed as a cellular thickening that arises from the center of the germ disc and moves centrifugally. This cumulus movement breaks the radial symmetry of the germ disc morphology, correlating with the development of the dorsal region of the embryo. Classical experiments on spider embryos have shown that a cumulus has the capacity to induce a secondary axis when transplanted ectopically. In this study, we have examined the house spider, Achaearanea tepidariorum, on the basis of knowledge from Drosophila to characterize the cumulus at the cellular and molecular level. In the cumulus,a cluster of about 10 mesenchymal cells, designated the cumulus mesenchymal(CM) cells, is situated beneath the epithelium, where the CM cells migrate to the rim of the germ disc. Germ disc epithelial cells near the migrating CM cells extend cytoneme-like projections from their basal side onto the surface of the CM cells. Molecular cloning and whole-mount in situ hybridization showed that the CM cells expressed a spider homolog of Drosophila decapentaplegic (dpp), which encodes a secreted protein that functions as a dorsal morphogen in the Drosophila embryo. Furthermore, the spider Dpp signal appeared to induce graded levels of the phosphorylated Mothers against dpp (Mad) protein in the nuclei of germ disc epithelial cells. Adding data from spider homologs of fork head,orthodenticle and caudal, we suggest that, in contrast to theDrosophila embryo, the progressive mesenchymal-epithelial cell interactions involving the Dpp-Mad signaling cascade generate dorsoventral polarity in accordance with the anteroposterior axis formation in the spider embryo. Our findings support the idea that the cumulus plays a central role in the axial pattern formation of the spider embryo.

Establishment of dorsoventral (DV) polarity is essential for bilaterians to shape their body. In the development of many bilaterians, the DV axis, which is orthogonal to the anteroposterior (AP) axis, becomes morphologically apparent through a chain of asymmetric cell movements and behaviors. In the development of the chelicerate spider embryo(Montgomery, 1909;Holm, 1940;Holm, 1952;Seitz, 1966), the first morphological DV asymmetry can be recognized by the formation and movement of the cumulus, which appears from the center of the symmetrical germ disc and moves straight to the rim. The cumulus is a cellular thickening that makes a bulge on the surface of the germ disc. The functional importance of the cumulus in early patterning of the spider embryo was demonstrated by Holm(Holm, 1952), who used embryos of the spider, Agelena labyrinthica. Extirpation of the cumulus produced abnormal embryos that lacked the dorsal area. By contrast,transplantation of a part of the cumulus to ectopic sites frequently resulted in twined embryos. Based on these data, Holm suggested that the cumulus, like the dorsal lip in amphibians, is an organizing center for the axial pattern formation of the spider embryo. Despite this fascinating idea, however, the cumulus has been poorly characterized in a cellular and molecular context.

Cellular and molecular mechanisms that control early patterning have been most extensively studied for the insect, Drosophila melanogaster. In the early Drosophila embryo, AP and DV asymmetries pre-exist as localized maternal components, which give rise to a region-specific zygotic expression of genes (St Johnston and Nüsslein-Volhard, 1992;Driever, 1993;Chasan and Anderson, 1993). The DV polarity of the Drosophila embryo comes from a regulated nuclear localization of the maternal transcription factor Dorsal. A gradient of nuclear Dorsal concentration along the DV axis subdivides the embryo into a series of domains with different fates: amnioserosa, dorsal ectoderm, ventral(neurogenic) ectoderm and mesoderm (Rusch and Levine, 1996; Stathopoulos and Levine, 2002). The boundaries of these domains are established by the activities of zygotic genes transcribed at different thresholds of the Dorsal gradient. One such zygotic gene is decapentaplegic(dpp), which is expressed in dorsal 40% of the cellular blastoderm(St Johnston and Gelbart,1987). It encodes a secreted protein belonging to the TGFβsuperfamily (Padgett et al.,1987). The dpp mutant embryo is strongly ventralized(Irish and Gelbart, 1987). Conversely, dpp overexpression expands the dorsal area(Ferguson and Anderson, 1992a;Ferguson and Anderson, 1992b). Thus, the Dpp protein acts as a dorsal morphogen in the Drosophilaembryo. In cells that receive the Dpp signal, the cytoplasmic Mothers against dpp (Mad) protein is phosphorylated by activated Dpp receptors, and translocated to the nucleus, where phosphorylated Mad (pMad) regulates transcription of downstream genes (Raftery and Sutherland, 1999; Rushlow et al., 2001). Another zygotic gene, short gastrulation(sog), is expressed at lateral regions next to thedpp-expressing domain(François et al.,1994). This gene encodes an extracellular protein(François et al., 1994)that antagonizes the Dpp activity(Marqués et al., 1997;Ashe and Levine, 1999). The dorsal region is established by the relative activities of Dpp, Sog and other factors (Ashe et al.,2000).

Striking similarities in the mechanisms of DV patterning are known betweenDrosophila and vertebrates. Vertebrate homologs of dpp andsog, BMP2/4 and chordin, respectively, function to organize the DV pattern in a similar fashion although the ventral side of vertebrates corresponds to the dorsal side of Drosophila(Holley et al., 1995;De Robertis and Sasai, 1996;Ferguson, 1996;Holley and Ferguson, 1997). Combined with the distant phylogenetic relationship betweenDrosophila and vertebrates, it is generally thought that the origin of the DV axis is shared by most bilaterians. However, there are very few studies investigating how the embryonic DV axis is specified in bilaterian animals other than insects and vertebrates. Although homologs ofdpp/BMP2/4 have been isolated in a wide range of metazoans, including the amphioxus, ascidian, sea urchin, gastropod, planarian and coral(Miya et al., 1997;Panopoulou et al., 1998;Orii et al., 1998;Angerer et al., 2000;Darras and Nishida, 2001;Hayward et al., 2002;Nederbragt et al., 2002),developmental analyses on these genes have not revealed plausible scenarios for the evolution of DV axis formation in the metazoans. It may be important to try to figure out the ancestral mode of DV patterning for the respective phyla or classes.

In non-insect arthropods, there is only limited knowledge concerning cellular and molecular mechanisms of early patterning. Molecular phylogenetics suggests that within the Arthropoda the Chelicerata are the living group phylogenetically most distant from the Insecta(Friedrich and Tautz, 1995;Hwang et al., 2001;Giribet et al., 2001;Cook et al., 2001). Comparative analysis is expected to provide data for understanding the ancestral developmental mechanisms of the arthropods. An increasing number of developmental genes have been isolated and examined in the spider and other chelicerates, illuminating similarities and differences betweenDrosophila and chelicerates(Telford and Thomas, 1998a;Telford and Thomas, 1998b;Damen and Tautz, 1998;Damen et al., 1998;Damen et al., 2000; Abzhanov et al., 1999; Stollewerk et al.,2001; Damen, 2002;Dearden et al., 2002).

In this study, in order to compare the mechanisms patterning the AP and DV axes of Drosophila and spider embryos, we used Achaearanea tepidariorum, which is easily accessible to analyzing the early embryogenesis. First, we describe a cluster of mesenchymal cells at the cumulus, which migrate from the center to the rim of the germ disc resulting in the transition from radial to axial symmetry. Next, we isolated spider genes homologous to Drosophila early patterning genes, dpp, fork head (fkh), orthodenticle (otd; oc —FlyBase) and caudal (cad), and examined their expressions. In the Drosophila cellular blastoderm, dpp is expressed to specify the dorsal part (Irish and Gelbart, 1987; St Johnston and Gelbart, 1987; Ferguson and Anderson, 1992a; Ferguson and Anderson, 1992b), and fkh, otd and cad are expressed to specify the anterior and posterior terminal domains(Weigel et al., 1989;Cohen and Jürgens, 1990;Finkelstein and Perrimon,1990; Macdonald and Struhl,1986; Wu and Lengyel,1998). The expression patterns of the spider genes helped understand the early development of the embryo. We found that the mesenchymal cells at the cumulus expressed the dpp homolog. Furthermore, using the crossreacting antibody against phosphorylated Mad (pMad), we showed that the Dpp signal was received by germ disc epithelial cells. We suggest that at the cumulus, progressive mesenchymal-epithelial cell interactions involving the Dpp-Mad signaling cascade generate DV polarity in accordance with the AP axis formation. Our findings offer molecular support for the functional importance of the cumulus in the axial pattern formation of the spider embryo.

### Spider

The cosmopolitan house spider, Achaearanea tepidariorum (Araneae,Theridiidae), was used. Spiders were originally collected on the campus of Kyoto University (Kyoto, Japan). They were cultured in the laboratory over four generations in cycles of light (15 hours) and dark (9 hours) at 25°C,and fed with flies and crickets. Egg sacs were kept in 100 mm dishes with wet cotton. Hatched larvae were kept in the same dishes until they became fourth or fifth instars. They were then individually transferred to small glass tubes(15×70 mm). Several days after the final ecdysis, females were transferred to larger cups and mated with mature males. Within several days,the females made an egg sac containing approximately 200-300 eggs, and repeated the egg laying approximately once a week for two months. Living embryos were observed in halocarbon oil 700 (Sigma).

### Scanning electron microscopy

Spider eggs were dechorionated with commercial bleach, followed by careful washing with distilled water. For fixation, the eggs were incubated for several hours or over night in a two-phase solution of heptane, 4%paraformaldehyde and 0.7% glutaraldehyde in C & G's balanced saline (55 mM NaCl, 40 mM KCl, 15 mM MgSO4, 5 mM CaCl2 and 10 mM Tricine, pH 6.9) at 4°C. The fixative was replaced by phosphate-buffered saline (PBS) with 0.1% Tween20 (PBS-Tween), and the vitelline membranes were removed with forceps and glass needles. Some of the samples, used to observe the inside of the egg, were cut by a razor blade, followed by a removal of the yolk. Then, the samples were post-fixed with 2.5% glutaraldehyde in PBS for 1 hour or longer. After several washes with PBS, they were dehydrated through an ethanol series, followed by a replacement with t-butylalcohol. They were then critical-point dried using a freeze-drying device, JFD-300 (JEOL),sputtered with an ion sputtering device, JFC-1500 (JEOL), and examined at 10 kV in a scanning electron microscope (SEM), JSM-5300LLV (JEOL). Photographs were taken with Polapan 572 (Polaroid).

### Phalloidin staining

Eggs were fixed in the same fixative as used for the SEM preparation, or in a two-phase fixative of heptane and 4% paraformaldehyde in PBS for several hours or overnight at 4°C. After the removal of the vitelline envelopes,the eggs were stained with 1 U/ml phalloidin-fluorescein (Molecular Probes). The eggs fixed in the gulutaryaldehyde-containing fixative were structurally well preserved, but stained weakly as compared with those fixed in the other. Some of the samples were counterstained with 1 μM TOTO-3 (Molecular probes). The stained samples were observed under a Zeiss Axiophoto 2 microscope equipped with a BioRad laser confocal system (MRC1024).

### cDNA cloning

To isolate spider homologs of the Drosophila genes, dpp, fkh,otd and cad, we initially performed degenerate PCR. Primers used for amplification of the genes were as follows:

• the dpp forward primer, 5′ga(t/c)gtnggntgg(a/g)a(t/c)ga(t/c)tgg 3′ (for amino acid sequence DVGW(N/D)DW);

• the dpp reverse primer, 5′ cg(a/g)cancc(a/g)canccnacnac 3′ (for amino acid sequence VVGCGCR);

• the fkh forward primer, 5′ca(t/c)gcnaa(a/g)ccnccnta(t/c)(a/t)(g/c) 3′ (for amino acid sequence HAKPPYS);

• the fkh reverse primer, 5′gg(a/g)tgna(a/g)n(g/c)(a/t)cca(a/g)(t/a)a 3′ (for amino acid sequence(F/Y)W(T/S)LHP);

• the otd forward primer, 5′gnta(t/c)ccnga(t/c)at(t/c/a)tt(t/c)atg 3′ (for amino acid sequence RYPDIFM);

• the otd reverse primer, 5′gcnc(t/g)nc(t/g)(a/g)tt(t/c)tt(a/g)aacca 3′ (for amino acid sequence WFKNRRA);

• the cad forward primer, 5′gg(t/c)aa(a/g)acn(a/c)gnacnaa(a/g)ga 3′ (for amino acid sequence GKTRTKD);

• and the cad reverse primer, 5′tc(t/c)ttngcnc(t/g)nc(t/g)(a/g)tt(t/c)tg 3′ (for amino acid sequence QNRRAKE).

cDNA prepared from segmentation stage embryos was used as a template for the PCR amplifications. The cycles of PCR for dpp were: one cycle of 95°C for 5 minutes, 55°C for 2 minutes 30 seconds and 72°C for 40 seconds; and 35 cycles of 95°C for 40 seconds, 55°C for 40 seconds and 72°C for 40 seconds. For amplification of fkh, otd andcad, the annealing temperatures were changed to 45°C, 50°C and 50°C, respectively. Amplified fragments were subcloned using a TA-cloning® kit dual promoter (Invitrogen) and sequenced. Among them,candidates for the spider homologs of the Drosophila genes were found.

For cDNA library construction, two pools of polyA+ RNA were prepared from three egg sacs containing embryos at different stages and just hatched prelarvae of Achaearanea tepidariorum using a QuickPrep™ Micro mRNA Purification Kit (Amersham Pharmacia Biotech). From each RNA pool, oligo-dT primed cDNA libraries were constructed using SuperScript™ Lamda System for cDNA Synthesis and Cloning (Gibco BRL) and Gigapack®III Gold Packaging Extract (Stratagene).

For library screening, digoxigenin (DIG)-labeled DNA probes for the PCR-amplified fragments were made using a PCR DIG Probe Synthesis Kit (Roche). The embryo and prelarvae cDNA libraries were screened with the probes in a high stringent condition to obtain full-length cDNAs for the candidate genes. Both strands of representative cDNA clones were sequenced and their open reading frames were determined. As shown in the text, the isolated genes were concluded to be A. tepidariorum orthologs of Drosophila dpp, fkh,otd and cad, which were designated At.dpp, At.fkh,At.otd and At.cad, respectively. The sequences are available from the DNA data bank of Japan (DDBJ) with the following Accession Numbers:At.dpp, AB096072; At.fkh, AB096073; At.otd,AB096074; At.cad, AB096075.

### Phylogenetic analysis

The deduced amino acid sequences of the C-terminal region of the Dpp protein, the winged-helix region of Fkh and the homeodomains of Otd and Cad were manually aligned with the corresponding amino acid sequences of proteins from other species that were found using the BLAST search. Aligned sequences were used to construct phylogenetic trees by the neighbor-joining method(Saitou and Nei, 1987) using PHYLIP.

### In situ hybridization and antibody staining

RNA probes for in situ hybridization were prepared using T7 RNA polymerase(Gibco BRL, or Stratagene) and DIG RNA Labeling Mix (Roche) according to the standard method. After dechorionation with bleach, embryos were fixed in a two-phase solution of heptane and 5.5% formaldehyde in PEMS (100 mM PIPES, 1 mM EDTA, 2 mM MgSO4, pH 6.9). After fixation, the embryos were washed with PBS-Tween, followed by gradual replacement with methanol, and then the vitelline membranes were removed with forceps and glass needles. Alternatively, the vitelline membranes were removed in PBS-Tween before replacement with methanol. The former procedure protected the yolk mass from destruction, but the cumulus was hardly visible in the resultant samples. To observe the cumulus carefully, we followed the latter procedure, which allowed us to remove the yolk mass with the germ disc preserved. In the resultant samples, the cumulus was easily visible even after staining. Hybridization,washes and detection were performed in the same way as those for theDrosophila embryos (Lehmann and Tautz, 1994). For antibody staining, embryos were prepared in the same way as for the in situ hybridization staining. PS1 antibody raised against the phosphorylated human Smad1 C-terminal peptide(Persson et al., 1998) was used at a dilution of 1:500 to detect pMad protein. For the secondary antibody, biotin-conjugated antirabbit IgG (Amersham) was used at a dilution of 1:200. For detection, the elite ABC peroxidase kit (Vectastain) was used. For simultaneous detection for At.dpp RNA and the pMad protein, the in situ hybridization staining was followed by the antibody staining.

### Early embryogenesis of Achaearanea tepidariorum

The following is a brief description of embryogenesis up to the early segmentation stage of a house spider, Achaearanea tepidariorum, based on observations of the living embryos, a time-lapse movie of the embryogenesis(see Movie 1),and examinations performed in the past(Montgomery, 1909;Holm, 1940;Holm, 1952;Seitz, 1966). Eight stages were defined (Fig. 1). The spider egg is spherical in shape (Fig. 1). The morphological appearance at earliest stages do not predict the future embryonic axis. At stage 1, the nuclei divide deep from the egg surface to increase in number (Fig. 1A). Stage 2 begins with the arrival of the cleavage energids,which are composed of the nuclei and cytoplasmic components, at the periphery of the egg (Fig. 1B). During this stage, the energids continue to divide synchronously, being distributed evenly along the egg surface (Fig. 1B,C). At stage 3, the energids begin to shift towards one side of the egg along the surface (Fig. 1D). Within 10 hours, most of the energids become settled in half of the egg (Fig. 1E), entering stage 4. The energids give rise to a germ disc of relatively uniform epithelial cells. Although when the cellularization is completed in the species used has not been examined, a previous work on a closely related spider species, Achaearanea japonica, showed that every nucleus was already enclosed with plasma membrane at the 16-blastomere stage(Suzuki and Kondo, 1995). At the center of the germ disc, a white spot is seen(Fig. 1E). This is traditionally called the primary thickening (or cumulus anterior), at which mesenchymal cells are located beneath the surface epithelium. We use the term primary thickening. A sign of the primary thickening is already visible at stage 3. At the primary thickening there is a slight indentation that is generally called the blastopore. We are not sure whether the mesenchymal cells arise from invagination through the blastopore. Stage 5 is the stage characterized by the cumulus. At the beginning of stage 5 the primary thickening becomes enlarged, and then a larger thickening appears from one side of the primary thickening (Fig. 1F). This larger thickening is the cumulus. The cumulus, which shows a bulge of the surface epithelium(Fig. 2A), shifts straight to the rim of the germ disc (Fig. 1G; see Movie 1). It takes about 6 hours for the cumulus to shift. However, the primary thickening becomes indistinct after the cumulus leaves the center of the germ disc (Fig. 1G). In about 75% of stage 4 embryos, the primary thickening was positioned almost at the center of the germ disc, whereas in the remaining 25%, it was positioned to one side of the center. In these cases, the direction of the cumulus movement was not correlated with the asymmetric position of the primary thickening. Stage 5 ends with the arrival of the cumulus at the rim of the germ disc. During stage 6, dynamic rearrangement of the surface epithelial cells takes place in the germ disc (Fig. 1H; see Movie 1)(Holm, 1940;Holm, 1952;Seitz, 1966). Peripheral cells migrate circumferentially toward the opposite side of the egg with respect to the disappearing cumulus (Fig. 1H), contributing to the development of the anterior region of the embryo. At stage 7, the germ disc is changed to a fan-like shape, and a metameric pattern begins to be morphologically recognized(Fig. 1I). The caudal lobe is prominent, which is derived from the cells around the center of the germ disc(see Movie 1)(Holm, 1940;Holm, 1952;Seitz, 1966). At stage 8, the germ disc is completely transformed to a germ band(Fig. 1J). Opisthosoma segments emerge one by one from the caudal lobe.

Fig. 1.

Fig. 1.

Fig. 2.

Morphology of the cumulus. The direction of the cumulus movement is shown by the largest arrows (A,B,D-F). (A) SEM image showing the surface view of a cumulus. The epithelium is bulged at the cumulus. (B) SEM image showing the inside view of a cumulus. Asterisks indicate CM cells, which extend lamellipodia-like processes (thick arrows). Note that germ disc epithelial cells extend thin, long cytoplasmic projections like cytonemes (thin arrows)from their basal side onto the surface of the CM cells. A single epithelial cell has a single projection. (C) SEM image showing the inside view of germ disc epithelial cells far from the cumulus in the same embryo as in B. No cytoneme-like projections are seen. (D,E) Laser scanning microscopy (LSM)images of a cumulus stained with phalloidin (green) and TOTO-3 (purple). A series of optical sections separated by intervals of 0.52 μm were obtained to cover the epithelial and mesenchymal layers of the cumulus. D and E were constructed by overlaying 16 and nine successive optical sections,respectively, selected from the data set. D focuses on the CM cells, and E on the interface between the CM cells and the germ disc epithelial cells. Lamellipodia-like processes abundant with F-actin ingress into the spaces between the lateral surfaces of the epithelial cells as indicated by arrows (a and b). (F) LSM image showing a sagittal section of a cumulus stained with phalloidin-fluorescein. Cytoplasmic projections extend from the basal side of the epithelial cells are seen (arrows). Scale bar: 10 μm in A-C; 20 μm in D-F.

Fig. 2.

Morphology of the cumulus. The direction of the cumulus movement is shown by the largest arrows (A,B,D-F). (A) SEM image showing the surface view of a cumulus. The epithelium is bulged at the cumulus. (B) SEM image showing the inside view of a cumulus. Asterisks indicate CM cells, which extend lamellipodia-like processes (thick arrows). Note that germ disc epithelial cells extend thin, long cytoplasmic projections like cytonemes (thin arrows)from their basal side onto the surface of the CM cells. A single epithelial cell has a single projection. (C) SEM image showing the inside view of germ disc epithelial cells far from the cumulus in the same embryo as in B. No cytoneme-like projections are seen. (D,E) Laser scanning microscopy (LSM)images of a cumulus stained with phalloidin (green) and TOTO-3 (purple). A series of optical sections separated by intervals of 0.52 μm were obtained to cover the epithelial and mesenchymal layers of the cumulus. D and E were constructed by overlaying 16 and nine successive optical sections,respectively, selected from the data set. D focuses on the CM cells, and E on the interface between the CM cells and the germ disc epithelial cells. Lamellipodia-like processes abundant with F-actin ingress into the spaces between the lateral surfaces of the epithelial cells as indicated by arrows (a and b). (F) LSM image showing a sagittal section of a cumulus stained with phalloidin-fluorescein. Cytoplasmic projections extend from the basal side of the epithelial cells are seen (arrows). Scale bar: 10 μm in A-C; 20 μm in D-F.

### Morphological characterization of CM cells

Germ discs with the migrating cumulus were observed from the inside by SEM. Mesenchymal cells were found to be present at a position corresponding to the epithelial cell bulge of the cumulus (Fig. 2B, asterisks). We designated these cells cumulus mesenchymal (CM)cells. The CM cells were rather loosely associated with each other and showed rufflings of plasma membrane like lamellipodia(Fig. 2B, thick arrows). F-actin staining visualized the lamellipodia-like projections, some of which ingressed into the spaces between the columnar epithelial cells(Fig. 2D,E). Using the samples stained for F-actin and DNA, the number of the CM cells were counted. The average number was 8.7±1.1 (n=17 embryos). In addition to the CM cells, a small number of mesenchymal cells existed, associated with the basal surface of the epithelium but distributed differently from the CM cells(not shown).

Marked differences in morphologies were also observed among the epithelial cells. The epithelial cells located in the trail of the CM cells extended long, thin cytoplasmic projections from their basal side onto the surface of the CM cells (Fig. 2B, thin arrows). The epithelial cells positioned very close to the CM cells had short,thick projections. These cytoplasmic projections were faintly detectable by phalloidin-fluorescein (Fig. 2F). These observations remind one of the cytonemes described inDrosophila larval imaginal discs(Morata and Basler, 1999;Ramírez-Weber and Kornberg,1999; Ramírez-Weber and Kornberg, 2000). In most other epithelial cells of the germ disc,no cytoneme-like projections were found(Fig. 2C).

### Cloning of spider homologs of Drosophila dpp, fkh, otd andcad

To search for developmental genes expressed in early spider embryos, we isolated cDNA clones for A. tepidariorum homologs of Drosophila dpp, fkh, otd and cad. Details for each cDNA clone are described below and in the Materials and Methods. Phylogenetic trees were constructed from the alignments shown in Fig. 3 (see Fig. S1).

Fig. 3.

Characterization of amino acid sequences deduced from spider cDNA clones,At.dpp, At.fkh, At.otd and At.cad. Conserved amino acid residues in alignments are highlighted. Gaps introduced to optimize the alignments are indicated by dashes (A,D). (A) Amino acid sequence of the C-terminal domain of At.Dpp aligned with known Dpp/BMP2/4 family proteins andDrosophila 60A and Screw proteins. (Database Accession Numbers:Dm.Dpp, U63857; Tc.Dpp, U63132; Sg.Dpp, AF374725; Bf.BMP2/4, AF068750;Hr.BMPb, D85464; DrBMP-2, AF072456; DrBMP-4, D49972; Dm.60A, M84795; Dm.Screw,U17573.) (B) Amino acid sequence of the winged-helix (forkhead) domain of At.Fkh aligned with known Fkh/HNF-3 family proteins and DrosophilaFD1 and FD3-FD5 proteins. (Database Accession Numbers: Dm.Fkh, J03177; Tc.Fkh,AF217810; Bf.HNF3-,1 X96519; Hr.HNF-3, AB007406; DrAxial, Z22762; Dm.FD1 and FD3-FD5, M96440 and M96442-M96444.) (C) Amino acid sequence of the homeodomain and C-terminal hexapeptide of At.Otd aligned with known Otd/Otx family proteins and Drosophila Otp, Prd and Gsb proteins. Dashes indicate the varied numbers of amino acid residues omitted. (Database Accession Numbers: Dm.Otd, X58983; Tc.Otd1 and 2, AJ223627 and AJ223614; Bf.Otx,AF043740; Ci.Otx, AF305499; DrOtx2, D26173; Dm.Otp, NM-079075; Dm.Prd, M14548;Dm.Gsb, NM-079139.) (D) Amino acid sequence of the homeodomain of At.Cad aligned with known Cad/Cdx family proteins and Drosophila Dfd, Scr and Antp proteins. (Database Accession Numbers: Dm.Cad, NM-134301; Bm.Cad,D16683; Tc.CadA and B AJ00542 and AJ005422; DrCad1, X66958, Dm.Dfd, X05136;Dm.Scr, X14475; Dm.Antp, M20704.) Abbreviated species are as follows: Dm,Drosophila melanogaster; Tc, Tribolium castaneum; Sg,Schistocerca gregaria; Bm, Bombyx mori; Bf,Branchiostoma floridae; Hr, Halocynthia roretzi; Ci,Ciona intestinalis; Dr, Danio rerio.

Fig. 3.

Characterization of amino acid sequences deduced from spider cDNA clones,At.dpp, At.fkh, At.otd and At.cad. Conserved amino acid residues in alignments are highlighted. Gaps introduced to optimize the alignments are indicated by dashes (A,D). (A) Amino acid sequence of the C-terminal domain of At.Dpp aligned with known Dpp/BMP2/4 family proteins andDrosophila 60A and Screw proteins. (Database Accession Numbers:Dm.Dpp, U63857; Tc.Dpp, U63132; Sg.Dpp, AF374725; Bf.BMP2/4, AF068750;Hr.BMPb, D85464; DrBMP-2, AF072456; DrBMP-4, D49972; Dm.60A, M84795; Dm.Screw,U17573.) (B) Amino acid sequence of the winged-helix (forkhead) domain of At.Fkh aligned with known Fkh/HNF-3 family proteins and DrosophilaFD1 and FD3-FD5 proteins. (Database Accession Numbers: Dm.Fkh, J03177; Tc.Fkh,AF217810; Bf.HNF3-,1 X96519; Hr.HNF-3, AB007406; DrAxial, Z22762; Dm.FD1 and FD3-FD5, M96440 and M96442-M96444.) (C) Amino acid sequence of the homeodomain and C-terminal hexapeptide of At.Otd aligned with known Otd/Otx family proteins and Drosophila Otp, Prd and Gsb proteins. Dashes indicate the varied numbers of amino acid residues omitted. (Database Accession Numbers: Dm.Otd, X58983; Tc.Otd1 and 2, AJ223627 and AJ223614; Bf.Otx,AF043740; Ci.Otx, AF305499; DrOtx2, D26173; Dm.Otp, NM-079075; Dm.Prd, M14548;Dm.Gsb, NM-079139.) (D) Amino acid sequence of the homeodomain of At.Cad aligned with known Cad/Cdx family proteins and Drosophila Dfd, Scr and Antp proteins. (Database Accession Numbers: Dm.Cad, NM-134301; Bm.Cad,D16683; Tc.CadA and B AJ00542 and AJ005422; DrCad1, X66958, Dm.Dfd, X05136;Dm.Scr, X14475; Dm.Antp, M20704.) Abbreviated species are as follows: Dm,Drosophila melanogaster; Tc, Tribolium castaneum; Sg,Schistocerca gregaria; Bm, Bombyx mori; Bf,Branchiostoma floridae; Hr, Halocynthia roretzi; Ci,Ciona intestinalis; Dr, Danio rerio.

#### At.dpp

The isolated cDNA clone encodes a polypeptide of 370 amino acids with a putative signal sequence and a putative proteolytic cleavage site(Panganiban et al., 1990)between 255 and 256 amino acid residues. The C-terminal 102 amino acid residues of the deduced protein shows 65% and 74% identity with the corresponding region of Drosophila Dpp and zebrafish BMP2,respectively, and lower percentages of identity with those ofDrosophila 60A (50% identity) and Screw (42% identity)(Fig. 3A) The topology of a phylogenetic tree constructed using amino acid sequences from the C-terminal regions of the TGFβ superfamily proteins strongly suggested that the isolated gene was an ortholog of Drosophila dpp. It was designated asAt.dpp.

#### At.fkh

Drosophila fkh is a winged-helix domain (forkhead domain)containing transcription factor expressed in several embryonic tissues such as hindgut, stomodeum and yolk (Weigel et al., 1989). The cDNA clone isolated from the spider predicts a protein of 406 amino acids which contains a winged-helix domain 82% identical to that of Drosophila Fkh and 83% to that of zebrafish Axial. The protein is more closely related to Drosophila Fkh than to otherDrosophila winged-helix domain-containing proteins(Fig. 3B). In addition to the winged-helix domain, two other short domains at the C termini, which were conserved in most of the known Fkh/HNF3 class proteins(Lai et al., 1991), were found in the spider protein (not shown). Phylogenetic analysis based on the winged-helix domains confirmed that the isolated gene was an ortholog ofDrosophila fkh. It was designate as At.fkh.

#### At.otd

Drosophila otd encodes a Paired-type homeodomain protein, which is expressed in the head region (Finkelstein et al., 1990; Cohen and Jürgens, 1990;Finkelstein and Perrimon,1990). The cDNA clone isolated from the spider encodes a protein of 303 amino acids that contains a homeodomain. This homeodomain closely resembles those of Drosophila and other animal Otd/Otx proteins, but less closely resembles those of Drosophila Orthopedia (Otp), Paired(Prd) and Gooseberry (Gsb) (Fig. 3C). The C-terminal sequence of six amino acids, LKFESL, has an affinity to the sequence, W(K/R)FQVL, which is found at the C termini of most of the known Otd/Otx proteins except insect Otd proteins. Phylogenetic analysis based on the homeodomains confirmed that the isolated gene was an ortholog of Drosophila otd. It was designated as At.otd.

Drosophila cad encodes a homeodomain protein that is expressed from the earliest stage of embryogenesis and is required for normal posterior development (Mlodzik et al.,1985; Macdonald and Struhl,1986; Mlodzik and Gehring,1987; Wu and Lengyel,1998). The cDNA isolated from the spider encodes a protein of 300 amino acids that contains a homeodomain. This homeodomain is highly similar to those of Drosophila and other animal Cad/Cdx proteins, but less similar to those of Drosophila Deformed (Dfd), Sex comb reduced (Scr)and Antennapedia (Antp) (Fig. 3D). Phylogenetic analysis based on the homeodomains confirmed that the isolated gene was an ortholog of Drosophila cad. It was designated as At.cad.

### Expression of dpp in the CM cells

We examined the expression patterns of the isolated genes in stage 4-7 embryos by whole-mount in situ hybridization. Transcripts for At.dppwere not detected in stage 4 embryos, but were detected in stage 5 embryos. The signal was seen as a spot in the germ disc. The spot of At.dppexpression, which was found at different positions from embryo to embryo(Fig. 4A-D), corresponded to the position of the cumulus. The variation is probably due to variation in the age of the embryos. Even in embryos with the cumulus positioned almost at the center of the germ disc, At.dpp transcripts were already detectable(Fig. 4A,B), although we are not sure whether the At.dpp signal was asymmetric or not with respect to the position of the blastopore. Magnified images showed thatAt.dpp transcripts were expressed in the CM cells, but not in the surface epithelial cells (Fig. 4E,F). The number of At.dpp-positive cells was 9.3±2.0 (n=16), which was comparable with the number of CM cells counted in the embryos stained for F-actin and DNA (see above).At.dpp transcripts were probably expressed in all the CM cells. At late stage 6, when the cumulus disappeared, the spot of At.dppexpression was not detected (not shown). At later stages, At.dppbegan to be expressed in developing limb buds(Fig. 4G), and the expression persisted at the extending limbs (Fig. 4H). This At.dpp expression resembles that ofdpp homologs in insect limbs(Sanchez-Salazar et al., 1996;Niwa et al., 2000;Dearden and Akam, 2001),suggesting a conserved function of dpp in limb bud formation between the insects and spiders.

Fig. 4.

Expression of At.dpp transcripts in stage 5 embryos revealed by whole-mount in situ hybridization. (A,B) Early stage 5 embryo viewed from the top (A) and the lateral side (B) of the germ disc. The signal is seen as a spot at the center of the germ disc. The rim of the germ disc is marked by dots in B as well as in C and D. (C) Mid stage 5 embryo. A spot of signal is seen at an intermediate position between the center and the rim of the germ disc. (D) Late stage 5 embryo. A spot of signal is seen close to the rim of the germ disc. The spots of dpp expression correspond to the positions of the cumulus in the embryos. (E,F) Close-up of the cumulus in a stage 5 embryo. E focuses on the surface epithelial cell layer and F on the CM cells. The signal is seen in the CM cells (F) but not the surface epithelial cells (E). (G,H) Embryos at about 65 hours (G) and 75 hours (H) AEL. The strong signal of At.dpp is seen in developing limb buds (G) and the tip of the extending limbs (H). The ventral midline is weakly stained (H). Ch,chelicerae; Pp, pedipalp; L1-4, leg segments 1-4.

Fig. 4.

Expression of At.dpp transcripts in stage 5 embryos revealed by whole-mount in situ hybridization. (A,B) Early stage 5 embryo viewed from the top (A) and the lateral side (B) of the germ disc. The signal is seen as a spot at the center of the germ disc. The rim of the germ disc is marked by dots in B as well as in C and D. (C) Mid stage 5 embryo. A spot of signal is seen at an intermediate position between the center and the rim of the germ disc. (D) Late stage 5 embryo. A spot of signal is seen close to the rim of the germ disc. The spots of dpp expression correspond to the positions of the cumulus in the embryos. (E,F) Close-up of the cumulus in a stage 5 embryo. E focuses on the surface epithelial cell layer and F on the CM cells. The signal is seen in the CM cells (F) but not the surface epithelial cells (E). (G,H) Embryos at about 65 hours (G) and 75 hours (H) AEL. The strong signal of At.dpp is seen in developing limb buds (G) and the tip of the extending limbs (H). The ventral midline is weakly stained (H). Ch,chelicerae; Pp, pedipalp; L1-4, leg segments 1-4.

### Localized phosphorylation of Mad in the epithelium

To identify the cells potentially responding to theAt.dpp-expressing CM cells in the spider embryo, we used the PS1 antibody, which was raised against the phosphorylated human Smad1 C-terminal peptide (Persson et al., 1998)and crossreacts with Drosophila pMad(Tanimoto et al., 2000). Staining with this antibody visualizes nuclei of the cells responding to the Dpp signal in Drosophila(Tanimoto et al., 2000;Rushlow et al., 2001;Dorfman and Shilo, 2001). In the staining of stage 5 spider embryos with PS1, specific signals were obtained in the germ disc epithelium. Only the epithelial cells located at and around the cumulus, but not the CM cells, showed stained nuclei(Fig. 5A,B). Accompanied by the cumulus, the pMad-positive region shifted in the germ disc epithelium. These indicated that the expression of nuclear pMad was transient in the epithelial cells. Double labeling for pMad and At.dpp transcripts confirmed that the region of Mad phosphorylation in the epithelium overlay theAt.dpp-expressing CM cells (Fig. 5C,D). There was a concentric gradient of Mad phosphorylation within the positive region, peaking at the epithelial cells closest to the CM cells (Fig. 5B-D).

Fig. 5.

Expression of pMad in stage 5 embryos. Embryos were stained with the PS1 antibody. (A) Mid stage 5 embryo viewed from the top of the germ disc. (B)Close-up of the same embryo as in A, flat-mounted. Nuclei of germ disc epithelial cells, but not CM cells, at and around the cumulus are stained. Note that the levels of signal are graded in a concentric manner. (C,D) Mid stage 5 embryo double-labeled for pMad (brown) and At.dpp transcripts(purple). C focuses on the surface epithelial cell layer and D on the CM cells. Note that the highest levels of pMad are observed just above the CM cells, expressing At.dpp transcripts.

Fig. 5.

Expression of pMad in stage 5 embryos. Embryos were stained with the PS1 antibody. (A) Mid stage 5 embryo viewed from the top of the germ disc. (B)Close-up of the same embryo as in A, flat-mounted. Nuclei of germ disc epithelial cells, but not CM cells, at and around the cumulus are stained. Note that the levels of signal are graded in a concentric manner. (C,D) Mid stage 5 embryo double-labeled for pMad (brown) and At.dpp transcripts(purple). C focuses on the surface epithelial cell layer and D on the CM cells. Note that the highest levels of pMad are observed just above the CM cells, expressing At.dpp transcripts.

### Expression of fkh in three different populations of cells

Expression of At.fkh was detected in three populations of cells. The first population was mesenchymal cells dispersed around the center of the germ disc at stage 5 (Fig. 6B-D). These cells probably derived from theAt.fkh-positive cells at the primary thickening at stage 4(Fig. 6A). Judging from their distribution patterns, these mesenchymal cells were different from the CM cells. The second population was cells at the rim of the germ disc(Fig. 6B,C,E,G). These cells appeared to migrate circumferentially and settle to the anterior region(Fig. 6H, part a) in a similar way to the At.otd-expressing cells (see below). The third population was surface epithelial cells at and around the cumulus approaching the rim of the germ disc at late stage 5 (Fig. 6E,F). During stage 6 and 7, the At.fkh-expressing cells spread over the yolk on the dorsal surface of the embryo(Fig. 6G,H, arrow; see Movie 1).

Fig. 6.

Expression of At.fkh transcripts revealed by whole-mount in situ hybridization. (A) Stage 4 embryo viewed from the top of the germ disc.fkh transcripts are found around the blastopore (white arrow). (B)Early stage 5 embryo. Expression of fkh transcripts are seen in cells dispersing from the center of the germ disc and in cells encircling the germ disc. (C,D) Mid-stage 5 embryo flat mounted. (D) High magnification of the boxed area in C, focused on the mesenchymal cell layer. TheAt.fkh-positive cells are mesenchymal cells located below the surface epithelial cell layer. (E,F) Late stage 5 embryo. The cumulus is magnified in F. In addition to the dispersing mesenchymal cells and the peripheral cells,expression of fkh transcripts is observed in epithelial cells at the cumulus (arrow in E). (G,H) Stage 6 (G) and 7 (H) embryos. Expression offkh transcripts is observed in the emerging dorsal region of the embryos (arrows), where the At.fkh-positive cells appear to spread over the yolk. The peripheral At.fkh-positive cells appear to migrate circumferentially to the emerging anterior region of the embryo (H, a). a,anterior; p, posterior; v, ventral.

Fig. 6.

Expression of At.fkh transcripts revealed by whole-mount in situ hybridization. (A) Stage 4 embryo viewed from the top of the germ disc.fkh transcripts are found around the blastopore (white arrow). (B)Early stage 5 embryo. Expression of fkh transcripts are seen in cells dispersing from the center of the germ disc and in cells encircling the germ disc. (C,D) Mid-stage 5 embryo flat mounted. (D) High magnification of the boxed area in C, focused on the mesenchymal cell layer. TheAt.fkh-positive cells are mesenchymal cells located below the surface epithelial cell layer. (E,F) Late stage 5 embryo. The cumulus is magnified in F. In addition to the dispersing mesenchymal cells and the peripheral cells,expression of fkh transcripts is observed in epithelial cells at the cumulus (arrow in E). (G,H) Stage 6 (G) and 7 (H) embryos. Expression offkh transcripts is observed in the emerging dorsal region of the embryos (arrows), where the At.fkh-positive cells appear to spread over the yolk. The peripheral At.fkh-positive cells appear to migrate circumferentially to the emerging anterior region of the embryo (H, a). a,anterior; p, posterior; v, ventral.

### Expression of otd in the future anterior region

In stage 5 embryos, transcripts for At.otd were expressed in the peripheral cells of the germ disc epithelium, three or four cells wide in a circle (Fig. 7A,B). At stage 6,when the germ disc began to be rearranged, At.otd expression was observed in an open' circle (Fig. 7C). This change in the pattern of At.otd expression appeared to be associated with the cumulus approaching(Fig. 7E,F), which might negatively regulate the expression and/or induce the circumferential migration of the At.otd-positive cells. By the end of stage 6, theAt.otd-expressing cells settled in the future head region(Fig. 7D).At.otd-negative cells, which possibly included theAt.fkh-positive cells described above(Fig. 6H), were present more anteriorly than the At.otd-positive cells.

Fig. 7.

Expression of At.otd and At.cad transcripts. Embryos stained by whole-mount in situ hybridization with the At.otd probe(A-D), At.otd and At.dpp mixed probes (E,F) orAt.cad probe (G-J). (A,B) Stage 5 embryo viewed from the top (A) and the lateral side (B) of the germ disc. Expression of At.otdtranscripts is observed in peripheral cells of the germ disc epithelium, three or four cells wide. (C) Stage 6 embryo viewed from the top of the germ disc. The pattern of At.otd expression is changed to an open' circle. (D)Stage 7 embryo viewed laterally. Expression of At.otd is seen at the emerging anterior region of the embryo. (E,F) Late stage 5 (E) and early stage 6 (F) embryos. The pattern of the At.otd-positive cells becomes theopen' circle at almost the same time when the CM cells expressingAt.dpp (arrowheads) reach the rim of the germ disc. (G,H) Stage 7 embryos viewed posteriorly (G) and laterally (H). Expression ofAt.cad transcripts is found at the most posterior region of the embryo corresponding to the caudal lobe. (I,J) High magnifications of the posterior end of a stage 7 embryo flat-mounted. I focuses on the surface of the embryo, and J on the inside. A pit is found at the center of theAt.cad-expressing region (arrow in I). Below the pit, there is a population of cells expressing At.cad transcripts (arrow in J). Mesenchymal cells not expressing At.cad transcripts are also found. The margin of the forming germ band is indicated by dots in D and H. a,anterior; p, posterior; d, dorsal; v, ventral.

Fig. 7.

Expression of At.otd and At.cad transcripts. Embryos stained by whole-mount in situ hybridization with the At.otd probe(A-D), At.otd and At.dpp mixed probes (E,F) orAt.cad probe (G-J). (A,B) Stage 5 embryo viewed from the top (A) and the lateral side (B) of the germ disc. Expression of At.otdtranscripts is observed in peripheral cells of the germ disc epithelium, three or four cells wide. (C) Stage 6 embryo viewed from the top of the germ disc. The pattern of At.otd expression is changed to an open' circle. (D)Stage 7 embryo viewed laterally. Expression of At.otd is seen at the emerging anterior region of the embryo. (E,F) Late stage 5 (E) and early stage 6 (F) embryos. The pattern of the At.otd-positive cells becomes the`open' circle at almost the same time when the CM cells expressingAt.dpp (arrowheads) reach the rim of the germ disc. (G,H) Stage 7 embryos viewed posteriorly (G) and laterally (H). Expression ofAt.cad transcripts is found at the most posterior region of the embryo corresponding to the caudal lobe. (I,J) High magnifications of the posterior end of a stage 7 embryo flat-mounted. I focuses on the surface of the embryo, and J on the inside. A pit is found at the center of theAt.cad-expressing region (arrow in I). Below the pit, there is a population of cells expressing At.cad transcripts (arrow in J). Mesenchymal cells not expressing At.cad transcripts are also found. The margin of the forming germ band is indicated by dots in D and H. a,anterior; p, posterior; d, dorsal; v, ventral.

### Expression of cad in the caudal lobe

No significant signal of At.cad expression was observed in stage 4-6 embryos. In stage 7 embryos, At.cad transcripts were found in the caudal lobe (Fig. 7G,H). At the center of the At.cad-expressing domain, a pit was present(Fig. 7I).At.cad-positive cells were also present underneath the pit(Fig. 7J), implying an invagination through the pit.

### Mesenchymal-epithelial cell interactions at the cumulus

In this work we have described the early embryogenesis of the spider with special attention to the cumulus. At the beginning of stage 5, the cumulus arose from the primary thickening (Fig. 1F). The cumulus was observed as a cellular thickening that consisted of clustered mesenchymal cells (CM cells) and germ disc epithelial cells (Fig. 2A,B). SEM and fluorescent microscopy visualized the physical interactions between the CM cells and the germ disc epithelial cells(Fig. 2), which may have contributed to the bulge formation. The CM cells migrated on the basal surface of the epithelium, whereas the germ disc epithelial cells appeared not to change their positions. Taken together, the movement of the cumulus indicates the migration of the clustered CM cells accompanied with the bulge formation of germ disc epithelial cells.

We showed that the CM cells, but not germ disc epithelial cells, expressedAt.dpp transcripts (Fig. 4E,F). Two lines of evidence suggest that the germ disc cells receive the Dpp signals from the CM cells. First, the nuclei of germ disc cells near the CM cells represented graded levels of pMad(Fig. 5). InDrosophila and vertebrates, phosphorylation and nuclear translocation of Mad/Smad proteins are shown to be caused by the activated specific receptors for Dpp/BMP ligands (Raftery and Sutherland, 1999). During migration, the CM cells were always centered in the pMad-positive circular region(Fig. 5), implying that the appearance of pMad in the germ disc epithelium depended on the CM cells. Second, the germ disc epithelial cells directly contacted the CM cells by using cytoneme-like projections, which were extended from the basal side of the epithelial cells onto the surface of the CM cells(Fig. 2B). InDrosophila larval imaginal discs, the cytonemes, which are projected from cells receiving signals, are thought to be the place for cell-cell communication (Morata and Basler,1999; Ramírez-Weber and Kornberg, 1999;Ramírez-Weber and Kornberg,2000). Similar to this case, the germ disc epithelial cells were likely to use cytoneme-like projections to receive the Dpp signals from the CM cells. Taken together, we suggest that the cumulus is a place for mesechymal-epithelial cell interactions that are involved in the pattern formation of the spider embryo.

Nuclear pMad/Smad drives transcription of specific downstream genes(Raftery and Sutherland, 1999;Rushlow et al., 2001). The expression of At.fkh in an area of the germ disc associated with the migrating CM cells (Fig. 6E,F)appeared to overlap that of pMad (Fig. 5). This suggested that At.fkh is a potential downstream target gene for the Dpp-Mad signaling cascade. The At.fkh-expressing cells at the cumulus, spreading over the yolk during stage 6 and 7(Fig. 6G,H), appeared to contribute to extra-embryonic tissue.

### Similarities and differences in body axis formation of theDrosophila and spider embryos

Expression patterns of spider homologs of Drosophilaregion-specific genes, shown in this study, offer molecular clues for determining homologous domains in the early spider and Drosophilaembryos (Fig. 8). In theDrosophila cellular blastoderm, otd is expressed at a region close to the anterior end (Finkelstein and Perrimon, 1990), whereas At.otd was expressed at a peripheral region of the germ disc encircled along the equator of the spider egg (Fig. 7A,B). Of theseAt.otd-expressing cells, cells around the cumulus might lack theAt.otd expression (see above), but the remainingAt.otd-expressing cells likely migrated circumferentially during stage 6 (Fig. 7C,F; see Movie 1)and settled to the anterior region of the germ band(Fig. 7D). Drosophila cad is expressed at a region close to the posterior end in the cellular blastoderm (Macdonald and Struhl,1986), whereas At.cad was expressed in the caudal lobe(Fig. 7G,H), which was derived from the central area of the germ disc (see Movie 1). These results, together with previous observations of spider embryos(Holm, 1940;Holm, 1952;Seitz, 1966), strongly suggest that the peripheral region of the germ disc corresponds to the anterior end of the Drosophila embryo, and the central region corresponds to the posterior end. It is possible that in the spider germ disc, the AP positional information pre-exists as a series of concentric circles. Based on this topology, At.fkh-expressing cells were located at the future anterior and posterior ends of the stage 5 embryo(Fig. 6B,C), similar to the pattern of fkh expression in the Drosophila cellular blastoderm (Weigel et al.,1989) and in the early embryo of other insects, Bombyxand Tribolium (Kokubo et al.,1996; Schröder et al.,2000). As the fkh-expressing cells become foregut and hindgut in these insects (Weigel et al.,1989; Kokubo et al.,1996; Schröder et al.,2000), the two populations of At.fkh-expressing cells are probably fated to be gut precursors.

Fig. 8.

Illustrations showing different modes of axial patterning of theDrosophila and spider embryo. The Drosophila cellular blastoderm stage (A) and the spider germ disc stage (B) are compared. a,anterior; p, posterior; v, ventral; d, dorsal. In each illustration, theotd- or At.otd-expressing anterior region of the embryo is indicated by yellow, and the posterior end of the embryo by purple. The area shown in gray indicate yolk (B). (A) In the Drosophila embryo,dpp is expressed in blastoderm cells at the 40% most dorsal region(green). This expression initiates simultaneously along the AP axis. In the same cell population that dpp is expressed, phosphorylation of Mad is induced. (B) In the spider embryo, future anterior cells (yellow) are located at the rim, whereas future posterior cells (purple) that form the caudal lobe exist around the center of the germ disc. The CM cells, which expressAt.dpp (green), migrate from the center to the rim of the germ disc(black arrow), progressively inducing phosphorylation of Mad in epithelial cells (light green). The area that once expressed pMad is shaded in green(right panel). It develops into the dorsal tissues. The site diametrically opposite to the cumulus is defined as the anterior pole (a, right panel). Broken line indicates the ventral midline; blue arrows indicate the directions of cell movement during stage 6.

Fig. 8.

Illustrations showing different modes of axial patterning of theDrosophila and spider embryo. The Drosophila cellular blastoderm stage (A) and the spider germ disc stage (B) are compared. a,anterior; p, posterior; v, ventral; d, dorsal. In each illustration, theotd- or At.otd-expressing anterior region of the embryo is indicated by yellow, and the posterior end of the embryo by purple. The area shown in gray indicate yolk (B). (A) In the Drosophila embryo,dpp is expressed in blastoderm cells at the 40% most dorsal region(green). This expression initiates simultaneously along the AP axis. In the same cell population that dpp is expressed, phosphorylation of Mad is induced. (B) In the spider embryo, future anterior cells (yellow) are located at the rim, whereas future posterior cells (purple) that form the caudal lobe exist around the center of the germ disc. The CM cells, which expressAt.dpp (green), migrate from the center to the rim of the germ disc(black arrow), progressively inducing phosphorylation of Mad in epithelial cells (light green). The area that once expressed pMad is shaded in green(right panel). It develops into the dorsal tissues. The site diametrically opposite to the cumulus is defined as the anterior pole (a, right panel). Broken line indicates the ventral midline; blue arrows indicate the directions of cell movement during stage 6.

One possibly important difference in the mode of AP axis formation is that in the spider embryo, it appears to occur in accordance with DV axis formation, whereas in the Drosophila embryo, the AP and DV axes are formed independently. Although a concentric series of AP positional information may pre-exist on the spider germ disc, the expression patterns of the AP patterning genes do not allow one to find the AP axis in the early germ disc. The anterior pole of the spider embryo can be defined only after the onset of the cumulus movement (Fig. 8).

Drosophila dpp and probably other insect dpp homologs are involved in DV patterning of the embryo. dpp is expressed in the dorsal ectoderm during the germband extending stages, and this expression probably contributes to pattern formation within segments, such as positioning of the limb buds (Sanchez-Salazar et al.,1996; Niwa et al.,2000; Dearden and Akam,2001). This role of dpp is probably conserved between the insects and spiders, as suggested by the later expression of At.dpp(Fig. 4G,H). At least inDrosophila, however, the earliest function of dpp is the specification of the DV pattern in the cellular blastoderm, not within segments of the germband (Ferguson and Anderson, 1992a; Rusch and Levine, 1996). The most dorsal region of the cellular blastoderm,where dpp is expressed at the strongest level, becomes extra-embryonic tissue (the amnioserosa), and the next regions become dorsal ectoderm in Drosophila. In the spider, the area of the germ disc that the At.dpp-expressing CM cells have directly influenced during their migration (Figs 1,2,5) develops into dorsal structures including the extra-embryonic tissue. These may suggest a conserved function of dpp for dorsal fate specification of the early embryo. The expression of At.dpp in the CM cells(Fig. 4) is probably comparable with the dorsal expression of dpp in the Drosophila cellular blastoderm, and might be related to the expression of dpp in an early cell population fated to be extra-embryonic tissue in more basal insect embryos (Sanchez-Salazar et al.,1996; Dearden and Akam,2001).

In the Drosophila blastoderm the expression of dpp, as well as some other zygotic genes involving DV patterning, is initiated in an asymmetric manner according to the gradient of the nuclear Dorsal protein peaking at the most ventral region (Rusch and Levine, 1996; Stathopoulos and Levine, 2002). In the spider embryo, however, the early detectable asymmetry was in the migration of the At.dpp-expressing CM cells rather than the expression of At.dpp itself. What mechanisms regulate the CM cell migration? The answer to this question may be the key to the developmental origin of the DV axis of the spider. A localized cue(s) that attracts or repulses the CM cells might pre-exist on the germ disc. Alternatively, the CM cells might sense only the AP positional information. In the latter case, the direction of the cell migration is determined at the start point randomly, or according to a local unevenness. Further molecular investigations are needed to find the earliest asymmetries potentially present in the germ disc and primary thickening.

Two important differences concern the Dpp signal betweenDrosophila and the spider (Fig. 8). First, the Dpp signal is produced and transduced within the surface epithelial cells in the Drosophila embryo(Dorfman and Shilo, 2001), in contrast to the spider embryo, in which the Dpp signal is produced by the mesenchymal cells (the CM cells) (Fig. 4), and is transmitted to the surface epithelial cells(Fig. 5). Second, the activation of the Dpp-Mad signaling pathway takes place simultaneously along the AP axis in the Drosophila embryo(Dorfman and Shilo, 2001), but took place progressively from the center to the rim of the germ disc in the spider embryo (Figs 4,5). These differences have implications for evolutionary change in the mechanism governing DV axis formation.

dpp/BMP2/4 class genes have been identified in non-chelicerate animals, including insects and non-arthropod invertebrates, and their expression patterns have been examined in early embryos(Sanchez-Salazar et al., 1996;Miya et al., 1997;Panopoulou et al., 1998;Angerer et al., 2000;Niwa et al., 2000;Darras and Nishida, 2001;Dearden and Akam, 2001;Hayward et al., 2002). However, of the cells expressing the dpp/BMP2/4 homologs in early embryos, none resembles the spider CM cells in morphology and behavior. Within the Chelicerata, the horseshoe crab(Sekiguchi, 1973), as well as many spider species (Montgomery,1909; Holm, 1940;Holm, 1952;Seitz, 1966), shows a cellular thickening similar to the cumulus in early embryogenesis. Even in early embryos of myriapod species, a cumulus-like cell mass has been reported(Heymons, 1901;Sakuma and Machida, 2002). In these cellular structures, cells that correspond to the CM cells described in this study might be present. Studies on dpp homologs will help to determine whether they are homologous to the spider cumulus. To understand the ancestral mode of DV patterning for the arthropods, the evolutionary and developmental origin of the CM cells is an important subject to be studied. For this purpose, crustaceans, which are suggested to be a sister group of insects (Friedrich and Tautz,1995; Hwang et al.,2001; Giribet et al.,2001; Cook et al.,2001), are not negligible. Early patterning of crustacean embryos at the cellular level is now being studied(Wolff and Scholtz, 2002;Gerberding et al., 2002).

### Regulative development of the early spider embryo

If it is assumed that common cellular and molecular mechanisms govern the early development of the spiders, Agelena labyrinthica andAchaearanea tepidariorum, the organizing activity of the cumulus demonstrated by Holm (Holm,1952) might be explained on the basis of our findings. He removed the cumulus resulting in ventralized embryos with the AP pattern retained to some extent. Loss of the source of Dpp signals might account for the ventralization. Transplanting a part of the cumulus ectopically resulted in twin embryos. Two moving sources of Dpp signals might set up two separate fields defined by positional values in the germ disc. Combining our findings and Holm's experimental data, it is strongly suggested that the cumulus plays a central role in the axial pattern formation of the spider embryo. Our molecular evidence clearly indicates that the spider cumulus is not homologous to the organizing center of vertebrate embryos, in which the activity of Sog/chordin, the Dpp/BMP2/4 antagonist, is dominant(Sasai et al., 1994). Studies on spider homologs of Sog/chordin are needed for a better understanding of DV patterning in the spider embryo.

The experiments by Holm (Holm,1952) and experiments on other spiders(Sekiguchi, 1957;Seitz, 1966;Seitz, 1970), indicate that the spiders adopt more regulative development than Drosophila. Like the spiders, the development of the horseshoe crab(Itow et al., 1991) and some insects (Sander, 1976) seems to be regulative. Probably in these arthropod species, the axial pattern formation largely relies on cell-cell interactions. In this study, we showed Dpp-mediated interactions between mesenchymal and epithelial cells at the cumulus. Probably, subsequent cell-cell interactions specify the fates of individual cells in the germ disc. Further investigations on patterning genes in the spider embryo will clarify the molecular basis for the regulative development of chelicerate embryos, which may give hints about the ancestral mode of arthropod development.

Movie and supplemental figure available online

We acknowledge K. Tanaka for advice on spider breeding, T. Tabata for providing us with PS1 antibody, A. Yoshida for technical instruction of SEM and M. Akam for comments on the manuscript. We thank M. Irie, M. Okubo and S. Okajima for technical assistance. We are also grateful to Sh. Tsukita, all the members of Tsukita Cell Axis Project and JT Biohistory Research Hall for helpful discussion and encouragement.

Abzhanov, A., Popadic, A. and Kaufman, T. C.(
2000
). Chelicerate Hox genes and the homology of arthropod segments.
Evol. Dev.
1
,
77
-89.
Angerer, L. M., Oleksyn, D. W., Logan, C. Y., McClay, D. R.,Dale, L. and Angerer, R. C. (
2000
). A BMP pathway regulates cell fate allocation along the sea urchin animal-vegetal embryonic axis.
Development
127
,
1105
-1114.
Ashe, H. L. and Levine, M. (
1999
). Local inhibition and long-range enhancement of Dpp signal transduction by Sog.
Nature
398
,
427
-431.
Ashe, H. L., Mannervik, M. and Levine, M.(
2000
). Dpp signaling thresholds in the dorsal ectoderm of theDrosophila embryo.
Development
127
,
3305
-3312.
Chasan, R. and Anderson, K. V. (
1993
). Maternal control of dorsal-ventral polarity and pattern in the embryo. In
The Development of Drosophila melanogaster
(ed. M. Bate and A. Martinez Arias), pp.
387
-424. New York:Cold Spring Harbor Laboratory Press.
Cohen, S. M. and Jürgens, G. (
1990
). Mediation of Drosophila head development by gap-like segmentation genes.
Nature
346
,
482
-485.
Cook, C. E., Smith, M. L., Telford, M. J., Bastianello, A. and Akam, M. (
2001
). Hox genes and the phylogeny of the arthropods.
Curr. Biol.
11
,
759
-763.
Damen, W. G. M. (
2002
). Parasegmental organization of the spider embryo implies that the parasegment is an evolutionary conserved entity in arthropod embryogenesis.
Development
129
,
1239
-1250.
Damen, W. G. M., Hausdorf, M., Seyfarth, E.-A. and Tautz, D.(
1998
). A conserved mode of head segmentation in arthropods revealed by the expression pattern of Hox genes in a spider.
Proc. Natl. Acad. Sci. USA
95
,
10665
-10670.
Damen, W. G. M. and Tautz, D. (
1998
). A Hox class 3 orthologue from the spider Cupiennius salei is expressed in a Hox-gene-like fashion.
Dev. Genes Evol.
208
,
586
-590.
Damen, W. G. M., Weller, M. and Tautz, D.(
2000
). Expression patterns of hairy, even-skipped, andrunt in the spider Cupiennius salei imply that these genes were segmentation genes in a basal arthropod.
Proc. Natl. Acad. Sci. USA
97
,
4515
-4519.
Darras, S. and Nishida, H. (
2001
). The BMP/CHORDIN antagonism controls sensory pigment cell specification and differentiation in the ascidian embryo.
Dev. Biol.
236
,
271
-288.
De Robertis, E. M. and Sasai, Y. (
1996
). A common plan for dorsoventral patterning in Bilateria.
Nature
380
,
37
-40.
Dearden, P. K. and Akam, M. (
2001
). Early embryo patterning in the grasshopper, Schistocerca gregaria:wingless, dacapentaplegic and caudal expression.
Development
128
,
3435
-3444.
Dearden, P. K., Donly, C. and Grbic, M. (
2002
). Expression of pair-rule gene homologues in a chelicerate: early patterning of the two-spotted spider mite Tetranychus urticae.
Development
129
,
5461
-5472.
Dorfman, R. and Shilo, B.-Z. (
2001
). Biphasic activation of the BMP pathway patterns the Drosophila embryonic dorsal region.
Development
128
,
965
-972.
Driever, W. (
1993
). Maternal control of anterior development in the Drosophila embryo. In
The Development of Drosophila melanogaster
(ed. M. Bate and A. Martinez Arias), pp.
301
-324. New York: Cold Spring Harbor Laboratory Press.
Ferguson, E. L. (
1996
). Conservation of dorsal-ventral patterning in arthropods and chordates.
Curr. Opin. Genet. Dev.
6
,
424
-431.
Ferguson, E. L. and Anderson, K. V. (
1992a
).decapentaplegic acts as a morphogen to organize dorsal-ventral pattern in the Drosophila embryo.
Cell
71
,
451
-461.
Ferguson, E. L. and Anderson, K. V. (
1992b
). Localized enhancement and repression of the activity of the TGF-β family member, decapentaplegic, is necessary for dorsal-ventral pattern formation in the Drosophila embryo.
Development
114
,
583
-597.
Finkelstein, R. and Perrimon, N. (
1990
). Theorthodenticle gene is regulated by bicoid and torsoand specifies Drosophila head development.
Nature
346
,
485
-488.
Finkelstein, R., Smouse, D., Capaci, T. M., Spradling, A. C. and Perrimon, N. (
1990
). The orthodenticle gene encodes a novel homeo domain protein involved in the development of theDrosophila nervous system and ocellar visual structures.
Genes Dev.
4
,
1516
-1527.
François, V., Solloway, M., O'Neill, J. W., Emery, J. and Bier, E. (
1994
). Dorsal-ventral patterning of theDrosophila embryo depends on a putative negative growth factor encoded by the short gastrulation gene.
Genes Dev.
8
,
2602
-2616.
Friedrich, M. and Tautz, D. (
1995
). Ribosomal DNA phylogeny of the major extant arthropod classes and the evolution of myriapods.
Nature
376
,
165
-167.
Gerberding, M., Browne, W. E. and Patel, N. H.(
2002
). Cell lineage analysis of the amphipod crustaceanParhyale hawaiensis reveals an early restriction of cell fates.
Development
129
,
5789
-5801.
Giribet, G., Edgecombe, G. D. and Wheeler, W. C.(
2001
). Arthropod phylogeny based on eight molecular loci and morphology.
Nature
413
,
157
-161.
Hayward, D. C., Samuel, G., Pontynen, P. C., Catmull, J., Saint,R., Miller, D. J. and Ball, E. E. (
2002
). Localized expression of a dpp/BMP2/4 ortholog in a coral embryo.
Proc. Natl. Acad. Sci. USA
99
,
8106
-8111.
Heymons, R. (
1901
). Die Entwicklungsgeschichte der Scolopender.
Zoologica (Stuttg.)
13
,
1
-244.
Holley, S. A. and Ferguson, E. L. (
1997
). Fish are like flies are like frogs: conservation of dorsal-venrtal patterning mechanisms.
BioEssays
19
,
281
-284.
Holley, S. A., Jackson, P. D., Sasai, Y., Lu, B., de Robertis,E. M., Hoffman, F. M. and Ferguson, E. L. (
1995
). A conserved system for dorsal-ventral patterning in insects and vertebrates involvingsog and chordin.
Nature
376
,
249
-253.
Holm, Å. (
1940
). Studien über die Entwicklung und Entwicklungsbiologie der Spinnen.
Zool. BiDr Uppsala
19
,
1
-214.
Holm, Å. (
1952
). Experimentelle Untersuchungen über die Entwicklung und Entwicklungsphysiologie des Spinnenembryos.
Zool. BiDr Uppsala
29
,
293
-424.
Hwang, U. W., Friedrich, M., Tautz, D., Park, C. J. and Kim,W. (
2001
). Mitochondrial protein phylogeny joins myriapods with chelicerates.
Nature
413
,
154
-157.
Irish, V. F. and Gelbart, W. M. (
1987
). The decapentaplegic gene is required for dorsal-ventral patterning of theDrosophila embryo.
Genes Dev.
1
,
868
-879.
Itow, T., Kenmochi, S. and Mochizuki, T.(
1991
). Induction of secondary embryos by intra- and interspecific grafts of center cells under the blastopore in horseshoe crabs.
Dev. Growth Differ.
33
,
251
-258.
Kokubo, H., Takiya, S., Mach, V. and Suzuki, Y.(
1996
). Spatial and temporal expression pattern of Bombyx fork head/SGF-1 gene in embryogenesis.
Dev. Genes Evol.
206
,
80
-85.
Lai, E., Prezioso, V. R., Tao, W., Chen, W. S. and Darnell Jr,J. E. (
1991
). Hepatocyte nuclear factor 3α belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head.
Genes Dev.
5
,
416
-427.
Lehmann, R. and Tautz, D. (
1994
). In situ hybridization to RNA. In Drosophila melanogaster:
Practical Uses in Cell and Molecular Biology
(ed. L. S. B. Goldstein and E. A. Fyrberg), pp.
575
-598. San Diego: Academic Press.
Macdonald, P. M. and Struhl, G. (
1986
). A molecular gradient in early Drosophila embryos and its role in specifying the body pattern.
Nature
324
,
537
-545.
Marqués, G., Musacchio, M., Shimell, M. J.,Wünnenberg-Stapleton, K., Cho, K. W. Y. and O'Connor, M. B.(
1997
). Production of a DPP activity gradient in the early Drosophila embryo through the opposing actions of the SOG and TLD proteins.
Cell
91
,
417
-426.
Miya, T., Morita, K., Suzuki, A., Ueno, N. and Satoh, N.(
1997
). Functional analysis of an ascidian homologue of vertebrate Bmp-2/Bmp-4 suggests its role in the inhibition of neural fate specification.
Development
124
,
5149
-5159.
Mlodzik, M., Fjose, A. and Gehring, W. J.(
1985
). Isolation of caudal, a Drosophila homeo box-containing gene with maternal expression, whose transcripts form a concentration gradient at the pre-blastoderm stage.
EMBO J.
4
,
2961
-2969.
Mlodzik, M. and Gehring, W. J. (
1987
). Expression of the caudal gene in the germ line of Drosophila:Formation of an RNA and protein gradient during early embryogenesis.
Cell
48
,
465
-478.
Montgomery, T. H., Jr (
1909
). The development of Theridium, an Aranead, up to the stage of reversion.
J. Morphol.
20
,
297
-352.
Morata, G. and Basler, K. (
1999
). Cells in search of a signal.
Nat. Cell Biol.
1
,
E60
-E61.
Nederbragt, A. J., van Loon, A. E. and Dictus, W. J. A. G.(
2002
). Expression of Patella vulgata orthorogs ofengrailed and dpp-BMP2/4 in adjacent domains during molluscan shell development suggests a conserved compartment boundary mechanism.
Dev. Biol.
246
,
341
-355.
Niwa, N., Inoue, Y., Nozawa, A., Saito, M., Misumi, Y., Ohuchi,H., Yoshioka, H. and Noji, S. (
2000
). Correlation of diversity of leg morphology in Gryllus bimaculatus (cricket) with divergence in dpp expression pattern during leg development.
Development
127
,
4373
-4381.
Orii, H., Kato, K., Agata, K. and Watanabe, K.(
1998
). Molecular cloning of Bone Morphogenetic Protein (BMP)gene from the planarian Dugesia japonica.
Zool. Sci.
15
,
871
-877.
Padgett, R. W., St Johnston, R. D. and Gelbart, W. M.(
1987
). A transcript from a Drosophila pattern gene predicts a protein homologous to the transforming growth factor-β family.
Nature
325
,
81
-84.
Panganiban, G. E. F., Rashka, K. E., Neitzel, M. D. and Hoffmann, F. M. (
1990
). Biochemical characterization of theDrosophila dpp protein, a member of the transforming growth factorβ family of growth factors.
Mol. Cell. Biol.
10
,
2669
-2677.
Panopoulou, G. D., Clark, M. D., Holland, L. Z., Lehrach, H. and Holland, N. D. (
1998
). AmpihBMP2/4, an amphioxus bone morphogenetic protein closely related to Drosophila decapentaplegic and vertebrate BMP2 and BMP4: Insights into evolution of dorsoventral axis specification.
Dev. Dyn.
213
,
130
-139.
Persson, U., Izumi, H., Souchelnytskyi, S., Itoh, S., Grimsby,S., Engström, U., Heldin, C.-H., Funa, K. and ten Dijke, P.(
1998
). The L45 loop in type I receptors for TGF-β family members is a critical determinant in specifying Smad isoform activation.
FEBS Lett.
434
,
83
-87.
Raftery, L. A. and Sutherland, D. J. (
1999
). TGF-β family signal transduction in Drosophila development: FromMad to Smads.
Dev. Biol.
210
,
251
-268.
Ramírez-Weber, F.-A. and Kornberg, T. B.(
1999
). Cytonemes: cellular processes that project to the principal signaling center in Drosophila imaginal discs.
Cell
97
,
599
-607.
Ramírez-Weber, F.-A. and Kornberg, T. B.(
2000
). Signaling reaches to new dimensions inDrosophila imaginal discs.
Cell
103
,
189
-192.
Rusch, J. and Levine, M. (
1996
). Threshold responses to the dorsal regulatory gradient and the subdivision of primary tissue territories in the Drosophila embryo.
Curr. Opin. Genet. Dev.
6
,
416
-423.
Rushlow, C., Colosimo, P. F., Lin, M.-c., Xu, M. and Kirov,N. (
2001
). Transcriptional regulation of theDrosophila gene zen by competing Smad and Brinker inputs.
Genes Dev.
15
,
340
-351.
Saitou, N. and Nei, M. (
1987
). The neighbor-joining method: A new method for reconstructing phylogenetic trees.
Mol. Biol. Evol.
4
,
406
-425.
Sakuma, M. and Machida, R. (
2002
). Germ band formation of a centipede Scolopocrytops rubiginosus L. Koch(Chilopoda: Scolopendromorpha).
Proc. Arthropod. Embryol. Soc. Jpn.
37
,
19
-23.
Sanchez-Salazar, J., Pletcher, M. T., Bennett, R. L., Brown, S. J., Dandamudi, T. J., Denell, R. E. and Doctor, J. S. (
1996
). The Tribolium decapentaplegic gene is similar in sequence, structure,and expression to the Drosophila dpp gene.
Dev. Genes Evol.
206
,
237
-246.
Sander, K. (
1976
). Specification of the basic body pattern in insect embryogenesis.
12
,
125
-238.
Sasai, Y., Lu, B., Steinbeisser, H., Geissert, D., Gont, L. K. and de Robertis, E. M. (
1994
). Xenopus chordin: A novel dorsalizing factor activated by organizer-specific homeobox genes.
Cell
79
,
779
-790.
Schröder, R., Eckert, C., Wolff, C. and Tautz, D.(
2000
). Conserved and divergent aspects of terminal patterning in the beetle Tribolium castaneum.
Proc. Natl. Acad. Sci. USA
97
,
6591
-6596.
Seitz, V. K.-A. (
1966
). Normale Entwicklung des Arachniden-Embryos Cupiennius salei Keyserling und seine Regulationsbefähigung nach Röntgenbestrahlungen.
Zool. Jb. Anat. Bd.
83
,
327
-447.
Seitz, V. K.-A. (
1970
). Embryonale Defekt- und Doppelbildungen im Ei der Spinne Cupiennius Salei (Ctenidae).
Zool. Jb. Anat. Bd.
87
,
588
-639.
Sekiguchi, K. (
1957
). Reduplication in spider eggs produced by centrifugation.
Sci. Rep. Tokyo Kyoiku Daigaku,Sec. B
8
,
227
-280.
Sekiguchi, K. (
1973
). A normal plate of the development of the Japanese horse-shoe crab, Tachypleus tridentatus.
Sci. Rep. Tokyo Kyoiku Daigaku, sec. B
15
,
153
-162.
St Johnston, D. and Nüsslein-Volhard, C.(
1992
). The origin of pattern and polarity in the Drosophila embryo.
Cell
68
,
201
-219.
St Johnston, R. D. and Gelbart, W. M. (
1987
). Decapentaplegic transcripts are localized along the dorsal-ventral axis of theDrosophila embryo.
EMBO J.
6
,
2785
-2791.
Stathopoulos, A. and Levine, M. (
2002
). Dorsal gradient networks in the Drosophila embryo.
Dev. Biol.
246
,
57
-67.
Stollewerk, A., Weller, M. and Tautz, D.(
2001
). Neurogenesis in the spider Cupiennius salei.
Development
128
,
2673
-2688.
Suzuki, H. and Kondo, A. (
1995
). Early embryonic development, including germ-disk stage, in the theridiid spiderAchaearanea japonica (Bös. et Str.).
J. Morphol.
224
,
147
-157.
Tanimoto, H., Itoh, S., ten Dijke, P. and Tabata, T.(
2000
). Hedgehog creates a gradient of DPP activity inDrosophila wing imaginal discs.
Mol. Cell
5
,
59
-71.
Telford, M. J. and Thomas, R. H. (
1998a
). Expression of homeobox genes shows chelicerate arthropods retain their deutocerebral segment.
Proc. Natl. Acad. Sci. USA
95
,
10671
-10675.
Telford, M. J. and Thomas, R. H. (
1998b
). Of mites and zen: expression studies in a chelicerate arthropod confirmzen is a divergent Hox gene.
Dev. Genes Evol.
208
,
591
-594.
Weigel, D., Jürgens, G., Küttner, F., Seifert, E. and Jäckle, H. (
1989
). The homeotic gene fork headencodes a nuclear protein and is expressed in the terminal regions of the Drosophila embryo.
Cell
57
,
645
-658.
Wolff, C. and Scholtz, G. (
2002
). Cell lineage,axis formation, and the origin of germ layers in the amphipod crustaceanOrchestia cavimana.
Dev. Biol.
250
,
44
-58.
Wu, L. H. and Lengyel, J. A. (
1998
). Role ofcaudal in hindgut specification and gastrulation suggests homology between Drosophila amnioproctodeal invagination and vertebrate blastopore.
Development
125
,
2433
-2442.