The Notch pathway mediates cell-to-cell communication in a variety of tissues, developmental stages and organisms. Pathway activation relies on the interaction between transmembrane ligands and receptors on adjacent cells. As such, pathway activity could be influenced by the size, composition or dynamics of contacts between membranes. The initiation of Notch signalling in the Drosophila embryo occurs during cellularization, when lateral cell membranes and adherens junctions are first being deposited, allowing us to investigate the importance of membrane architecture and specific junctional domains for signaling. By measuring Notch dependent transcription in live embryos we established that it initiates while lateral membranes are growing and that signalling onset correlates with a specific phase in their formation. However, the length of the lateral membranes per se was not limiting. Rather, the adherens
ACCEPTED MANUSCRIPT
RESEARCH ARTICLE|
06 September 2021
Membrane architecture and adherens junctions contribute to strong Notch pathway activation
Julia Falo-Sanjuan
,
Julia Falo-Sanjuan
Department of Physiology, Development and Neuroscience, University of Cam- bridge, Downing Street, Cambridge CB2 3DY
, UK
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Sarah J. Bray
Sarah J. Bray
*
Department of Physiology, Development and Neuroscience, University of Cam- bridge, Downing Street, Cambridge CB2 3DY
, UK
*Author for correspondence: sjb32@cam.ac.uk
Search for other works by this author on:
Julia Falo-Sanjuan
Department of Physiology, Development and Neuroscience, University of Cam- bridge, Downing Street, Cambridge CB2 3DY
, UK
Sarah J. Bray
*
Department of Physiology, Development and Neuroscience, University of Cam- bridge, Downing Street, Cambridge CB2 3DY
, UK
*Author for correspondence: sjb32@cam.ac.uk
Received:
27 May 2021
Accepted:
24 Aug 2021
Online ISSN: 1477-9129
Print ISSN: 0950-1991
Funding Group:
- Award Group:
- Funder(s): Wellcome Trust
- Award Id(s): 212207/Z/18/Z
- Funder(s):
Funding Group:
- Award Group:
- Funder(s): Medical Research Council
- Award Id(s): MR/T014156/
- Funder(s):
© 2021. Published by The Company of Biologists Ltd
2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Development dev.199831.
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Received:
27 May 2021
Accepted:
24 Aug 2021
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Julia Falo-Sanjuan, Sarah J. Bray; Membrane architecture and adherens junctions contribute to strong Notch pathway activation. Development 2021; dev.199831. doi: https://doi.org/10.1242/dev.199831
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