Gamete formation from germline stem cells (GSCs) is essential for sexual reproduction. However, the regulation of GSC differentiation are incompletely understood. Set2, which deposits H3K36me3 modifications, is required for GSC differentiation during Drosophila oogenesis. We discovered that the H3K36me3 reader Male-specific lethal 3 (MSL3) and histone acetyltransferase complex Ada2a-containing (ATAC) cooperate with Set2 to regulate GSC differentiation in female Drosophila. MSL3, acting independent from the rest of the male specific lethal complex, promotes transcription of genes including a germline enriched ribosomal protein S19 paralog, RpS19b. RpS19b upregulation is required for translation of RNA-binding Fox protein 1 (Rbfox1), a known meiotic cell cycle entry factor. Thus, MSL3 regulates GSC differentiation by modulating translation of a key factor that promotes transition to an oocyte fate.
ACCEPTED MANUSCRIPT
RESEARCH ARTICLE|
08 December 2021
MSL3 promotes germline stem cell differentiation in female Drosophila
In collection:
Reproductive biology
Alicia McCarthy
,
Alicia McCarthy
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
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Kahini Sarkar,
Kahini Sarkar
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
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Elliot T. Martin,
Elliot T. Martin
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
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Maitreyi Upadhyay,
Maitreyi Upadhyay
‡
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
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Seoyeon Jang,
Seoyeon Jang
2
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390
, USA
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Nathan D. Williams,
Nathan D. Williams
§
2
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390
, USA
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Paolo E. Forni,
Paolo E. Forni
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
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Michael Buszczak,
Michael Buszczak
2
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390
, USA
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Prashanth Rangan
Prashanth Rangan
*
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
*Author for correspondence and Lead Contact: prangan@albany.edu
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Alicia McCarthy
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
Kahini Sarkar
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
Elliot T. Martin
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
Maitreyi Upadhyay
‡
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
Seoyeon Jang
2
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390
, USA
Nathan D. Williams
§
2
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390
, USA
Paolo E. Forni
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
Michael Buszczak
2
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390
, USA
Prashanth Rangan
*
1
Department of Biological Sciences/RNA Institute, University at Albany SUNY, Albany, NY 12202
, USA
*Author for correspondence and Lead Contact: prangan@albany.edu
‡
Current address: Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138
§
Current address: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
Received:
29 Mar 2021
Accepted:
19 Nov 2021
Online ISSN: 1477-9129
Print ISSN: 0950-1991
Funding Group:
- Award Group:
- Funder(s): National Institutes of Health
- Award Id(s): R01GM111770
- Funder(s):
Development dev.199625.
Article history
Received:
29 Mar 2021
Accepted:
19 Nov 2021
Currently Viewing Accepted Manuscript - Newer Version Available
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Citation
Alicia McCarthy, Kahini Sarkar, Elliot T. Martin, Maitreyi Upadhyay, Seoyeon Jang, Nathan D. Williams, Paolo E. Forni, Michael Buszczak, Prashanth Rangan; MSL3 promotes germline stem cell differentiation in female Drosophila. Development 2021; dev.199625. doi: https://doi.org/10.1242/dev.199625
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