Canoe/Afadin and the GTPase Rap1 specify the subapical domain during cellularization in Drosophila embryos. The timing of domain formation is unclear. The subapical domain may gradually mature or emerge synchronously with basal and lateral domain. The mechanism for activation of Rap1 by potential guanyl nucleotide exchange factors (GEF) or GTPase activating proteins (GAP) is unknown. Here, we retraced the emergence of the subapical domain at the onset of cellularization by in vivo imaging with CanoeYFP in comparison to the lateral and basal markers, ScribbledGFP and CherrySlam. CanoeYFP accumulates at a subapical position at about the same time as the lateral marker ScribbledGFP but a few minutes prior to basal CherrySlam. Furthermore, we show that the unconventional GEF complex ELMO-Sponge is subapically enriched and is required for subapical restriction of Canoe. The localization dynamics of ELMO-Sponge suggests a patterning mechanism for positioning the subapical region adjacent to the apical region. While marking the disc-like apical regions before cellularization, ELMO-Sponge redistributes to a ring-like pattern surrounding the apical region at the onset of cellularization.
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RESEARCH ARTICLE|
01 January 2017
ELMO and Sponge specify subapical restriction of Canoe and formation of the subapical domain in early Drosophila embryos
Anja Schmidt,
Anja Schmidt
#
Institute for Developmental Biochemistry, University of Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany
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Zhiyi Lv,
Zhiyi Lv
#
Institute for Developmental Biochemistry, University of Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany
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Jörg Großhans
Jörg Großhans
*
Institute for Developmental Biochemistry, University of Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany
*Author for correspondence: joerg.grosshans@med.uni-goettingen.de
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Anja Schmidt
#
Institute for Developmental Biochemistry, University of Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany
Zhiyi Lv
#
Institute for Developmental Biochemistry, University of Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany
Jörg Großhans
*
Institute for Developmental Biochemistry, University of Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany
*Author for correspondence: joerg.grosshans@med.uni-goettingen.de
#
equal contribution
Received:
30 Jul 2017
Accepted:
18 Dec 2017
Online ISSN: 1477-9129
Print ISSN: 0950-1991
Funding
Deutsche Forschungsgemeinschaft
(UE 194/1-1)
Development dev.157909.
Article history
Received:
30 Jul 2017
Accepted:
18 Dec 2017
Currently Viewing Accepted Manuscript - Newer Version Available
26 Jan 2018
Citation
Anja Schmidt, Zhiyi Lv, Jörg Großhans; ELMO and Sponge specify subapical restriction of Canoe and formation of the subapical domain in early Drosophila embryos. Development 2017; dev.157909. doi: https://doi.org/10.1242/dev.157909
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