During development, precerebellar neurons migrate tangentially from the dorsal hindbrain to the floor plate. Their axons cross it but their cell bodies stop their ventral migration upon reaching the midline. It was previously shown, that Slit chemorepellents and their receptors Robo1 and Robo2 might control the migration of precerebellar neurons in a repulsive manner. Here we have used a conditional knockout strategy in mice to test this hypothesis. We show that the targeted inactivation of the expression of Robo1/2 receptors in precerebellar neurons does not perturb their migration and that they still stop at the midline. The selective ablation of the expression of all three Slits in floor plate cells has no effect on pontine neurons and only induces the migration of a small subset of inferior olivary neurons across the floor plate. Likewise, we show that the expression of Slits in the facial nucleus is dispensable for pontine neuron migration. Together, these results show that Robo1/2 receptors act non-cell autonomously in migrating precerebellar neurons, and that floor plate signals, other than Slits, should exist to prevent midline crossing.
ACCEPTED MANUSCRIPT
RESEARCH ARTICLE|
01 January 2017
Non-cell autonomous control of precerebellar neuron migration by Slits and Robos
In collection:
Neural development
Chloé Dominici,
Chloé Dominici
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France
Search for other works by this author on:
Quentin Rappeneau,
Quentin Rappeneau
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France
Search for other works by this author on:
Pavol Zelina,
Pavol Zelina
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France
Search for other works by this author on:
Stéphane Fouquet,
Stéphane Fouquet
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France
Search for other works by this author on:
Alain Chédotal
Alain Chédotal
*
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France
*Author for correspondence: alain.chedotal@inserm.fr;
Search for other works by this author on:
Chloé Dominici
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France
Quentin Rappeneau
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France
Pavol Zelina
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France
Stéphane Fouquet
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France
Alain Chédotal
*
Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Institut de la Vision, 17 Rue Moreau, 75012 Paris, France
*Author for correspondence: alain.chedotal@inserm.fr;
Received:
08 Feb 2017
Accepted:
11 Dec 2017
Online ISSN: 1477-9129
Print ISSN: 0950-1991
Funding
Fondation pour la Recherche Médicale
(DPC20111123002)
Agence Nationale de la Recherche
(ANR-14-CE13-0004-01)
Development dev.150375.
Article history
Received:
08 Feb 2017
Accepted:
11 Dec 2017
Currently Viewing Accepted Manuscript - Newer Version Available
17 Jan 2018
Citation
Chloé Dominici, Quentin Rappeneau, Pavol Zelina, Stéphane Fouquet, Alain Chédotal; Non-cell autonomous control of precerebellar neuron migration by Slits and Robos. Development 2017; dev.150375. doi: https://doi.org/10.1242/dev.150375
Download citation file:
