In Arabidopsis, rapid expansion of the coenocytic endosperm after fertilization has been proposed to drive early seed growth, which is in turn constrained by the seed coat. This hypothesis implies physical heterogeneity between the endosperm and seed coat compartments during early seed development, which to date has not been demonstrated. Here we combine tissue indentation with modelling to show that the physical properties of the developing seed are consistent with the hypothesis that elevated endosperm-derived turgor pressure drives early seed expansion. We provide evidence that whole-seed turgor is generated by the endosperm at early developmental stages. Furthermore, we show that endosperm cellularization and seed growth arrest are associated with a drop in endosperm turgor pressure. Finally we demonstrated that this decrease is perturbed when the function of POLYCOMB REPRESSIVE COMPLEX2 is lost, suggesting that turgor pressure changes could be a target of genomic imprinting. Our results indicate a developmental role for changes in endosperm turgor-pressure in the Arabidopsis seed.
ACCEPTED MANUSCRIPT
RESEARCH REPORT|
01 January 2016
Endosperm turgor pressure decreases during early Arabidopsis seed development
In collection:
Plant development
Léna Beauzamy,
Léna Beauzamy
+
1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France
Search for other works by this author on:
Chloé Fourquin,
Chloé Fourquin
+
1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France
Search for other works by this author on:
Nelly Dubrulle,
Nelly Dubrulle
1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France
Search for other works by this author on:
Yann Boursiac,
Yann Boursiac
2
Biochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche 5004, CNRS/INRA/Montpellier SupAgro/Université de Montpellier, Montpellier, France
Search for other works by this author on:
Arezki Boudaoud,
Arezki Boudaoud
*
1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France
Search for other works by this author on:
Gwyneth Ingram
Gwyneth Ingram
*
1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France
Search for other works by this author on:
Léna Beauzamy
+
1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France
Chloé Fourquin
+
1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France
Nelly Dubrulle
1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France
Yann Boursiac
2
Biochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche 5004, CNRS/INRA/Montpellier SupAgro/Université de Montpellier, Montpellier, France
Arezki Boudaoud
*
1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France
Gwyneth Ingram
*
1
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France
+
These authors contributed equally to the manuscript.
Received:
07 Mar 2016
Accepted:
24 May 2016
Online ISSN: 1477-9129
Print ISSN: 0950-1991
Association Nationale de la Recherche et de la Technologie
(ANR-10-CHEX-0011)
European Research Council
(CDREG, 32998)
Institut National de la Recherche Agronomique
(CESETAB)
© 2016. Published by The Company of Biologists Ltd
2016
Development dev.137190.
Article history
Received:
07 Mar 2016
Accepted:
24 May 2016
Currently Viewing Accepted Manuscript - Newer Version Available
15 Sep 2016
Citation
Léna Beauzamy, Chloé Fourquin, Nelly Dubrulle, Yann Boursiac, Arezki Boudaoud, Gwyneth Ingram; Endosperm turgor pressure decreases during early Arabidopsis seed development. Development 2016; dev.137190. doi: https://doi.org/10.1242/dev.137190
Download citation file: