There were errors published in Development133, 2371-2381.
The authors have become aware of errors with the display of data in Fig. 4E, Fig. 5A and Fig. 6. These errors are detailed below, and the authors have also provided the original data for these figures, which are included as part of the correction.
In Fig. 4E, the Rnf2 and trimH3-K27 lanes were spliced together without marking, removing intervening lanes from the original gel. In preparing the figure, the Hoxb6 Rnf2 lanes were duplicated as Hoxb8 Rnf2. In addition, contrast adjustments were applied to the original image that obscured weak bands in the Rnf2–/– lanes that we assume are the result of non-specific binding of chromatin to the beads during the immunoprecipitation procedure. Finally, the control lanes are wrongly labelled as Hprt; the negative control used for this experiment was in fact Adam34. These errors are corrected in the revised Fig. 4 and legend shown below, and the original data for this experiment are provided as ‘Fig. 4E original data’.
Fig. 4 (revised)
In Fig. 5A, for all samples except Hoxa4, the order in which the lanes are shown in the figure does not represent the order in which they were run on the original gel, where samples were run in the order: Suz12, Eed3, Rnf2, trimH3-K27, Input. For Hoxb1 and Hoxb4, the lanes were exchanged in the figure but this splicing was not appropriately marked. For Hoxb6 and Hoxb8, we failed to exchange these lanes and therefore the labelling was incorrect in the published figure. In addition, the control lanes were wrongly labelled as Hprt; the negative control used was Adam34. These errors are corrected in the revised Fig. 5 and legend shown below, and the original data for this experiment are provided as ‘Fig. 5A original data’.
Fig. 5 (revised)
In Fig. 6A, the original experiment included samples taken from Rnf110+/–; Bmi1–/– animals. These were removed from the published version, but this splicing was not marked. In Fig. 6B, the original experiment included samples taken from Phc1–/–; Phc2+/–. We chose to show only data from the double heterozygote and double homozygote mutant, but in the case of Hoxb8 accidentally removed the Phc1+/–; Phc2+/– lanes instead of Phc1–/–; Phc2+/–. As can be seen in the original data, the results are highly similar for both genotypes. These errors are corrected in the revised Fig. 6 and legend shown below, and the original data for these experiments are provided as ‘Fig. 6 original data’.
Fig. 6 (revised)
The authors apologise to readers for these errors. The editors of the journal and the authors’ Research Integrity Office have examined this paper in detail and find that, although there were substantive errors in data presentation, these do not affect the conclusions of the paper.
Fig. 4E original data
Without lane splicing:
From laboratory notebook:
Fig. 5A original data
Without lane splicing:
From laboratory notebook:
Fig. 6 original data
Without lane splicing:
