Glia play crucial roles in ensheathing axons, a process that requires an intricate series of glia-neuron interactions. The membrane-anchored protein Wrapper is present in Drosophila midline glia and is required for ensheathment of commissural axons. By contrast, Neurexin IV is present on the membranes of neurons and commissural axons, and is highly concentrated at their interfaces with midline glia. Analysis of Neurexin IV and wrapper mutant embryos revealed identical defects in glial migration,ensheathment and glial subdivision of the commissures. Mutant and misexpression experiments indicated that Neurexin IV membrane localization is dependent on interactions with Wrapper. Cell culture aggregation assays and biochemical experiments demonstrated the ability of Neurexin IV to promote cell adhesion by binding to Wrapper. These results show that neuronal-expressed Neurexin IV and midline glial-expressed Wrapper act as heterophilic adhesion molecules that mediate multiple cellular events involved in glia-neuron interactions.

During development, CNS axons undergo a series of pathway choices to find their synaptic targets, and in the vertebrate spinal cord most axons cross the midline in commissures before joining longitudinal axon tracts. The floorplate cells of the spinal cord reside along the ventral midline, where they closely associate with commissural axons (Campbell and Peterson, 1993) and extend processes that subdivide axon bundles (Yoshioka and Tanaka,1989). Like the floorplate cells, the midline glia (MG) of the Drosophila CNS are centrally located and serve as a source of secreted factors required for attracting axons to the midline and ensuring that they do not recross (Garbe and Bashaw, 2004). In each segment, the MG ensheath the anterior (AC)and posterior (PC) commissures; each individual commissure is then further subdivided into axon-containing regions separated by MG projections(Noordermeer et al., 1998; Stollewerk and Klambt, 1997). The process of commissural axon ensheathment resembles vertebrate myelination,requiring that the glia migrate towards, recognize, adhere to and completely surround the axonal processes. These similarities make the DrosophilaMG an attractive system for studying the development and function of neuron-glia interactions.

Initially, there are ten MG composed of two classes: anterior midline glia(AMG) and posterior midline glia (PMG)(Dong and Jacobs, 1997; Kearney et al., 2004; Wheeler et al., 2006). The AMG initially consist of six cells. These cells migrate towards, make contact with and ensheath the developing axon commissures through a series of stereotyped movements and process extensions. On average, only three AMG closely contact the commissural axons, resulting in their continued survival, while the remaining AMG undergo apoptosis (Bergmann et al., 2002). All PMG die via apoptosis, and do not contribute to the mature MG (Dong and Jacobs,1997; Sonnenfeld and Jacobs,1995). Genetic studies have shown that the EGF, FGF and PVF signaling pathways are involved in aspects of MG development(Jacobs, 2000; Learte et al., 2008). However,members of these pathways are unlikely to mediate the adhesive interactions that underlie MG-neuron interactions. Analysis of the immunoglobulin (Ig)superfamily member wrapper showed that it is highly expressed in MG and is required for MG-neuron adhesion, as well as for the proper ensheathment and subdivision of the axon commissures(Noordermeer et al., 1998). One key issue is the identity of the binding partner on neuronal membranes that functions with Wrapper in MG-neuron adhesion.

A strong candidate is Neurexin IV (Nrx-IV), which is expressed throughout the CNS and encodes a transmembrane protein containing four extracellular Laminin G domains and two EGF domains(Baumgartner et al., 1996). While distantly related to other vertebrate and invertebrate Neurexin proteins, Drosophila Nrx-IV is orthologous to vertebrate Caspr(paranodin; Cntnap1) (Banerjee et al.,2006b). Caspr is localized to paranodal axo-glial junctions of myelinated neurons, where it binds to the Ig superfamily proteins contactin and neurofascin (Bhat, 2003; Charles et al., 2002). Drosophila Nrx-IV is also localized to septate junctions at axo-glial interfaces, and interacts with Contactin and Neuroglian(Banerjee et al., 2006a), which is highly related to neurofascin.

Since Nrx-IV is expressed in neurons and binds to Ig superfamily members, we tested the hypothesis that Nrx-IV and Wrapper physically interact and mediate MG-neuron interactions. Using a sim-Gal4 UAS-tau-GFPmidline cell marker strain (Wheeler et al., 2006) we were able to carefully examine midline cell morphology, movement and axonal ensheathment during embryonic development. Genetic analysis of Nrx-IV mutants revealed defects in MG-neuron and MG-axon interactions that are identical to those observed with wrapper mutants (Noordermeer et al., 1998). Nrx-IV protein was highly localized to the interface between MG and neuronal surfaces (both axon and cell body). The localization of Nrx-IV on neuronal membranes was dependent on the presence of Wrapper, and immunoprecipitation experiments demonstrated a physical interaction. Using cultured Drosophila S2 cells, we showed that mixing wrapper-and Nrx-IV-transfected cells resulted in cellular aggregation, and this effect was dependent upon the presence of both proteins. As in embryos,the Nrx-IV present in the aggregated cells was highly localized at sites of cell-cell contact. Thus, Nrx-IV and Wrapper function as heterophilic adhesion molecules that mediate MG migration and the ensheathment and subdivision of commissural axons.

Drosophila strains

Drosophila strains used were: ap-Gal4(Calleja et al., 1996), arm-Gal4 (Sanson et al.,1996), en-Gal4 (Ward et al., 1998a), Nrx-IV4304(Baumgartner et al., 1996), Nrx-IV-GFP (CA06597) (Buszczak et al., 2007; Laval et al.,2008; Morin et al.,2001), sim-Gal4 (Xiao et al., 1996), slit-Gal4(Scholz et al., 1997), UAS-GFP-lacZ.nls (Shiga et al.,1996), UAS-tau-GFP(Brand, 1995), UAS-wrapper (Noordermeer et al.,1998) and wrapper175(Noordermeer et al.,1998).

In situ hybridization, immunostaining and immunoprecipitation

Embryo collection, in situ hybridization, immunostaining and immunoprecipitation were performed as previously described(Kearney et al., 2004; Banerjee et al., 2006a). Primary antibodies used were: mouse MAb BP102 (Developmental Studies Hybridoma Bank, DSHB), rat anti-Elav MAb 7E8A10 (DSHB), mouse anti-En MAb 4D9(Patel et al., 1989), chicken anti-GFP (Upstate), rabbit anti-GFP Ab290 (Abcam), guinea pig anti-Lim3(Broihier and Skeath, 2002),rabbit anti-Nrx-IV (Baumgartner et al.,1996), mouse anti-Nrt MAb BP106 (DSHB), guinea pig anti-Runt (East Asian Distribution Center) (Kosman et al.,1998), mouse anti-Wrapper MAb 10D3 (DSHB)(Noordermeer et al., 1998) and guinea pig anti-Wrapper. Generation of the guinea pig anti-Wrapper utilized a 6×His-tagged fusion protein containing amino acids 245-444 of Wrapper as immunogen. Midline cells were examined in abdominal segments A1-8. Owing to the three-dimensional structure of the midline cells, it was difficult to represent all relevant cells in a single focal plane; so, for clarity,irrelevant portions of single images within a stack of confocal images were subtracted before projections were generated.

Cell culture, RNAi and immunofluorescence

Cell culture and RNAi experiments were performed as described(Rogers and Rogers, 2008). wrapper (pAc-wrapper) and Nrx-IV(pAc-Nrx-IV) open reading frames were PCR amplified from full-length cDNA clones and cloned into the pAc-V5/His A vector (Invitrogen) providing expression induced by the constitutively active Actin 5C promoter(Han et al., 1989). For immunofluorescence experiments, cells were plated onto poly-lysine-coated coverslips 24 hours after transfection, fixed, and stained with antibodies against Nrx-IV and Wrapper. All experiments were performed in duplicate and more than 100 cells were analyzed for each sample. Control cells were transfected with pMt-GFP to assess background aggregation. For cell aggregation assays, S2 cells were transfected with either pAc-wrapperor pAc-Nrx-IV by electroporation (Amaxa Nucleofector, Lonza). After 24 hours, the cells were resuspended, mixed, gently rocked on a Nutator for 1 hour, and plated onto poly-lysine-treated coverslips. For RNAi experiments,cells were treated daily with 10 μg dsRNA for 6 days followed by transfection with pAc-wrapper for 1 day. Negative controls were conducted with dsRNA generated against pBluescript vector, whereas controls to ensure that cells were competent for RNAi treatment were conducted with dsRNA targeted to Rho1 (Rogers and Rogers, 2008). Nrx1 and Nrx2 dsRNAs were generated by PCR of an Nrx-IV cDNA with two primer sets: set 1,5′-TAATACGACTCACTATAGGGGTCAATAATGGCATTAAATCGGTGCAAGCAGACG-3′ and 5′-TAATACGACTCACTATAGGCCCAGTTCAGTGCTGTCTTTCATCACTCG-3′; set 2,5′-TAATACGACTCACTATAGGGCACAGTACTATATTGCTGGTGGAAAGGACAAAAATGG-3′and 5′-TAATACGACTCACTATAGGGCATCGGCAATGCGGAAGGTCACCACATTAC-3′.

Imaging MG migration and axonal ensheathment in sim-Gal4 UAS-tau-GFP embryos

We have recently employed a sim-Gal4 UAS-tau-GFP transgenic strain and confocal microscopy to study the development of Drosophila CNS midline cells (Wheeler et al.,2006; Wheeler et al.,2008). In sim-Gal4 UAS-tau-GFP embryos, GFP is localized to the cytoplasm of all midline cells - both neurons and glia(Wheeler et al., 2006). When examined in sagittal views, this allows visualization of the morphology of each midline cell type during development. Our identification of specific midline cell types employed immunostaining or in situ hybridization with more than 90 validated cell type-specific reagents(Wheeler et al., 2006; Wheeler et al., 2008). In this paper, we use this system to investigate the dynamics of MG development during embryonic stages 12-17 in both wild-type and mutant embryos. MG were identified by their distinct shape, relatively dorsal position within the CNS and expression of wrapper, which is high in AMG, low in PMG, and absent from neurons (Noordermeer et al.,1998; Wheeler et al.,2006). PMG were additionally identified by expression of engrailed (en). Antibodies recognizing all neurons(anti-Elav), their axons (MAb BP102), and the midline precursor 1 (MP1)neurons (anti-Lim3) provided a comprehensive view of MG interactions with nearby neurons and axons. At the beginning of CNS axonogenesis [stages 12/3 to 12/0; subdivisions of stage 12 according to Klambt et al.(Klambt et al., 1991)],commissural axons initially converged into a single axon bundle(Fig. 1A,B). At stage 12/3, the AMG resided in the anterior of the segment and had an elongated morphology as they migrated toward the axon commissure(Fig. 1A). Approximately three AMG made contact with the anterior surface of the commissure while the remaining AMG underwent apoptosis, in keeping with published observations(Bergmann et al., 2002). At stage 12/0, the AMG sent processes across both the dorsal and ventral surfaces of the commissure (Fig. 1B). As the commissure separated into AC and PC, the AMG membranes completely ensheathed the AC (Fig. 1C). AC ensheathment concluded with the movement of an AMG cell body between the commissures (Fig. 1D). After the AC was completely ensheathed, a single, dorsally located AMG migrated across the dorsal surface of the PC during stages 15-16(Fig. 1E). This AMG extended processes posteriorly to ensheath the PC(Fig. 1F). During stages 15-17,the AMG also sent cytoplasmic projections into both the AC and PC that became more elaborate as development proceeded(Fig. 1E,F). Using electron microscopy, Stollewerk and Klambt(Stollewerk and Klambt, 1997)showed that these cytoplasmic projections divide each commissure into distinct subdomains.

Both the MP1 neurons and PMG were also in proximity to AMG and the commissures. Their positions were constant relative to the migrating AMG. The MP1 neurons remained in close contact with the ventral-most AMG from stages 11-17 (Fig. 1B-F; see Fig. S1A-D in the supplementary material), and prior to commissure separation the MP1 neurons were closely associated with the commissure along its ventral side(Fig. 1B; see Fig. S1A in the supplementary material). The PMG migrated dorsally and in an anterior direction during stage 12, and at least one PMG abutted the posterior side of the PC from stages 12-16 (Fig. 1A-E; see Fig. S1E-H in the supplementary material) before undergoing apoptosis (Bergmann et al.,2002; Sonnenfeld and Jacobs,1995). The cell bodies of non-midline-derived neurons also made extensive contacts with the AMG (see Fig. 4D) and, together with the PMG and MP1 neurons, they might play important roles in AMG development. Overall, the view of MG development described above is in general agreement with that described by others(Jacobs, 2000), validating the use of sim-Gal4 UAS-tau-GFP to study MG development. However, there are some important differences in nomenclature and PMG migration (see Discussion).

Nrx-IV mutants have disrupted MG-neuron interactions

During an earlier analysis of the role of Nrx-IV in septate junctions (Banerjee and Bhat,2007; Baumgartner et al.,1996), a failure of commissure separation was observed in Nrx-IV mutants (Fig. 2A,B). Previously, disruption of the commissures was correlated with MG defects (Klambt et al.,1991). To investigate a potential Nrx-IV midline phenotype, Nrx-IV4304, a null allele, was used in combination with sim-Gal4 UAS-tau-GFP to examine the dynamics of AMG migration, axonal ensheathment and commissure subdivision. During stage 12,the migration of the AMG and their juxtaposition to the unseparated commissure were normal (Fig. 2C). Once the AMG contacted the commissure, they sent processes across the AC as in the wild type (Fig. 2D). However,instead of ensheathing the AC, both the dorsally and ventrally located AMG migrated past the AC towards the PC (compare Fig. 2E,F with Fig. 1C,D). Strikingly, the AMG were frequently dissociated from the MP1 neurons residing at a more distant and dorsal location away from the midline neurons(Fig. 2H,I). This phenotype was the most common defect observed in stage 17 Nrx-IV mutant segments(Table 1). In stage 17 wild-type embryos, 3.1±0.4 AMG (n=33 segments) were present,whereas in embryos with dissociated AMG this was reduced to 2.1±0.5 AMG(n=28 segments). The reduction was likely to be due to the lack of AMG ensheathment of the PC, and the corresponding inability of the AMG to receive a sufficient axon-derived survival signal(Bergmann et al., 2002). Additional Nrx-IV mutant phenotypes were: (1) dissociation with incomplete migration and ensheathment of the AC and PC(Fig. 2J; Table 1), and (2) a complete absence of AMG (Fig. 2K; Table 1). In all mutant segments, the AMG failed to extend glial projections to subdivide either the AC or PC (Fig. 2A,B; and compare Fig. 2I with Fig. 1F). The inability of the AMG to properly migrate along the AC coupled with their inability to send projections into the commissures indicated that Nrx-IV was required for interactions between AMG and commissural axons. Furthermore, the dissociation of AMG from the MP1 neurons indicated that interactions between these two cell types are important for AMG positioning.

Nrx-IV protein is present in neurons and localizes to MG-neuron interfaces

To understand how mutations in Nrx-IV lead to defects in AMG development, the CNS expression and protein localization of Nrx-IVwas analyzed in wild-type embryos by in situ hybridization and immunostaining. Transcripts were detected at a low level throughout the CNS(Fig. 3A; see Fig. S1I-K in the supplementary material), with no increase in expression levels at or near the midline. This was in sharp contrast to Nrx-IV immunostaining, which showed high levels of protein at the midline (Fig. 3B,C) (Baumgartner et al.,1996). We argue that the Nrx-IV midline staining is due to Nrx-IV neuronal expression and protein localization at MG-neuron interfaces, and is absent from MG (or present at insignificant levels).

There were two major locations at which Nrx-IV was concentrated(Fig. 3C): (1) along the axon commissures, and (2) at the boundaries between MG and neurons. At stages 12/0 to 13, Nrx-IV was found at the interface where the migrating AMG contacted the single, unseparated commissure (Fig. 3D). After commissure separation and ensheathment, Nrx-IV localized to the boundaries where commissures and AMG were juxtaposed and along the AMG projections that subdivided the commissures(Fig. 3E).

Nrx-IV was localized at detectable, but low, levels around the membranes of neurons (Fig. 3B), and at high levels at sites of contact between lateral CNS neurons and AMG(Fig. 3F). Neurons also showed Nrx-IV localization where they contacted PMG, but the accumulation was weaker than with AMG (Fig. 3C). Nrx-IV was highly localized to the contacts between AMG and the MP1 neurons(Fig. 3G); this localization was prominent from stage 12/3 until the MP1 neurons underwent apoptosis during stage 17 (Miguel-Aliaga and Thor,2004). To further investigate the subcellular localization of Nrx-IV, we examined a protein-trap GFP fusion of Nrx-IV (Nrx-IV-GFP). The localization of Nrx-IV-GFP strongly resembled endogenous Nrx-IV protein with respect to cell type localization: it was present in neurons but absent or at low levels in MG (Fig. 3H,I). Although Nrx-IV-GFP localized at MG-neuron interfaces, it was less pronounced than endogenous Nrx-IV. Conversely, the Nrx-IV-GFP was more cytoplasmic than Nrx-IV and its localization was enhanced in the axon scaffold(Fig. 3I). Neither Nrx-IV immunostaining nor Nrx-IV-GFP was observed at appreciable levels at the membranes of AMG or PMG that were not contacting neurons(Fig. 3C,H). These data suggest that Nrx-IV is expressed in neurons and not MG.

To test the requirement for Nrx-IV in neurons or glia, we used a neural driver (elav-Gal4) and an MG driver (slit-Gal4) to express UAS-Nrx-IV in a protein-negative Nrx4304-null mutant and assayed Nrx-IV protein localization and rescue of the Nrx-IV MG migration defects. In elav-Gal4 rescue embryos, Nrx-IV was localized at the boundaries between neurons and both AMG and PMG (compare Fig. 3J with 3B), similar to wild type. Unlike in Nrx-IV mutants, in these rescue embryos AMG ensheathed the AC by stage 15 and migrated between the AC and PC(Fig. 3K-M; Table 2). However, by late stage 17, the AMG failed to ensheath the PC or to send projections into either commissure (data not shown). Thus, misexpression of Nrx-IV using elav-Gal4 was able to rescue the initial steps of MG migration, but not the later aspects. We noted that Nrx-IV protein levels were lower in the rescue embryos from stages 15 to 17 as compared with wild-type embryos (data not shown), a result consistent with the decrease in elav-Gal4expression reported previously (Lin and Goodman, 1994). This might explain the lack of full rescue late in development. slit-Gal4 rescue embryos did not show localization of Nrx-IV at MG-neuron boundaries (see Fig. S1L,N in the supplementary material)and the Nrx-IV mutant MG migration defects were not rescued either at stage 15 or 17 (Table 2; see Fig. S1L-O in the supplementary material). In summary, only when expressed in neurons did Nrx-IV accumulate at MG-neuron interfaces and partially rescue the Nrx-IV mutant phenotype.

Wrapper localizes to MG membranes and wrapper MG mutant phenotypes are identical to those of Nrx-IV

From stages 11-17, wrapper is expressed at high levels in AMG and at low levels in PMG (see Fig. S1E-H in the supplementary material)(Noordermeer et al., 1998; Wheeler et al., 2006). Unlike Nrx-IV, wrapper in situ hybridization showed strong MG expression(Fig. 4A). Wrapper protein was first observed at stage 12/3 and was present throughout AMG migration and ensheathment. The protein was uniformly localized on MG membranes(Fig. 4B), including where they contacted MP1 neurons (Fig. 4C), commissural axons (Fig. 4C,E), lateral CNS neuronal cell bodies(Fig. 4D) and other MG(Fig. 4B). At later stages,Wrapper was present on AMG projections during commissure subdivision(Fig. 4E).

Genetic analysis of wrapper175-null mutant embryos revealed MG phenotypes that were identical to Nrx-IV mutant embryos,and to the wrapper phenotypes reported previously using electron microscopy (Noordermeer et al.,1998). At stage 12, the AMG and PMG of wrapper mutants migrated dorsally and contacted the commissures normally. Defects appeared after the AMG contacted the AC. By stage 15, AMG failed to ensheath the AC,and an AMG cell body was not interposed between the AC and PC(Fig. 4F). The wrappermutant phenotype was highly penetrant: 99% of stage 17 segments possessed defects (n=139) (Table 1). In 76% of mutant segments, AMG surrounded the AC but failed to surround the PC (Fig. 4G), and in the other 24% of mutant segments, the AMG failed to surround either the AC or PC (Fig. 4H). In all segments examined, the AMG were dissociated from the MP1s(Fig. 4I) and failed to extend projections to subdivide the commissures(Fig. 4G). As in Nrx-IV mutants, the number of AMG present during stage 17 was reduced, from 3.1±0.4 (n=33) in wild type to 2.3±0.7(n=14). Thus, wrapper is required for AMG migration,commissural ensheathment and commissure subdivision. Phenotypes of Nrx-IV wrapper double mutants were similar to, but more severe than, either single mutant: the same percentage of segments showed defects as in the single mutants, but more segments showed incomplete ensheathment of either the AC or PC (Table 1). One interpretation of the more severe double-mutant phenotype is that both Nrx-IV and Wrapper might be interacting with additional proteins on the apposing cell.

Nrx-IV membrane localization is dependent on Wrapper

In wild-type embryos, Nrx-IV protein was present at low levels throughout the membranes of neuronal cell bodies and axons, but was highly concentrated where they contacted AMG (Fig. 3B; Fig. 5A). The similarity of the Nrx-IV and wrapper mutant phenotypes coupled with the concentration of Nrx-IV opposite Wrapper+MG membranes led us to hypothesize that the high-level membrane localization of Nrx-IV was dependent on the presence of Wrapper on the apposing membranes. To investigate this possibility, Nrx-IV localization was examined in wrapper mutant and misexpression embryos. In wrappermutants, Nrx-IV was not concentrated at the contact points of neurons with MG,but was instead distributed uniformly around neuronal cell bodies(Fig. 5A,B) and axons(Fig. 5C,D) at higher levels than observed in wild-type embryos.

To determine whether Nrx-IV localization could be influenced by misexpressing wrapper, en-Gal4 was used to express UAS-wrapper in a subset of CNS cells. In en-Gal4 UAS-GFP-lacZ.nls control embryos, cells that contacted GFP+cells did not show concentrations of Nrx-IV at their membranes(Fig. 5E). However, when wrapper was misexpressed in these cells, the Wrapper+cells induced high levels of adjacent Nrx-IV(Fig. 5F). Similar results were observed when UAS-wrapper was misexpressed in a different set of cells using apterous-Gal4 (data not shown). These data indicated that Wrapper has the ability to induce Nrx-IV membrane localization on adjacent cell membranes. Interestingly, Nrx-IV accumulation only occurred in Wrapper- cells that bordered Wrapper+ cells; Nrx-IV did not accumulate at high levels between adjacent Wrapper+ cells(Fig. 5F). The ectopic neuronal Wrapper protein appeared more punctate than MG Wrapper, whereas the apposing Nrx-IV appeared relatively uniform. However, close examination(Fig. 5F) showed that Wrapper was also uniformly present along the membrane at low levels in addition to the presence of puncta. Nevertheless, the apparent difference in Wrapper levels between MG (endogenous Wrapper) and neurons (ectopic Wrapper) might indicate the existence of co-factors present in MG, but absent from neurons, that stabilize Wrapper at the membrane or prevent its degradation.

Nrx-IV and Wrapper proteins physically interact

The cellular and genetic experiments described above strongly suggested that Nrx-IV and Wrapper interact at the MG-neuron interface. To test this hypothesis, we carried out immunoprecipitation experiments from embryonic lysates using both Nrx-IV and Wrapper antibodies(Fig. 6A). Since wrapper is expressed in only a small number of embryonic cells,immunoprecipitation experiments were carried out in an arm-Gal4 UAS-wrapper strain, in which wrapper is expressed at high levels. Under these conditions, anti-Wrapper immunoprecipitated Nrx-IV, and anti-Nrx-IV immunoprecipitated Wrapper. Together with the localization of the proteins, these data strongly suggest that Nrx-IV and Wrapper directly bind at the MG-neuron interface.

Nrx-IV and Wrapper interact to induce cell adhesion in S2 cells

To test the hypothesis that Nrx-IV and Wrapper act as heterophilic cell adhesion partners, S2 cells were used to assay cell adhesion(Hortsch and Bieber, 1991). Immunoblot analysis of S2 cell extracts revealed that Nrx-IV was abundantly present as a 155 kDa protein (Fig. 6B); by contrast, Wrapper was undetectable (data not shown). S2 cells were transfected at high concentration with pAc-wrapper and pAc-Nrx-IV. Transfection of Nrx-IV alone did not result in the formation of aggregates (Fig. 6D), whereas transfection of wrapper alone resulted in the formation of small aggregates (2-15 cells/aggregate)(Fig. 6E). Mixing together of Nrx-IV- and wrapper-transfected cells resulted in the appearance of large aggregates (>100 cells/aggregate)(Fig. 6F), indicating that Nrx-IV and Wrapper bind in trans and mediate cell adhesion.

Since transfection of wrapper alone in S2 cells induced small aggregates, we tested whether this aggregation was due to the presence of endogenous Nrx-IV or a different adhesion molecule. RNAi was used to remove endogenous Nrx-IV in cells transfected with wrapper alone. Two distinct dsRNAs (Nrx1 and Nrx2) each depleted Nrx-IV protein levels by more than 95% as compared with control levels(Fig. 6B). S2 cells were then treated with either Nrx-IV or control dsRNAs, transfected with wrapper, and assayed for aggregation(Fig. 6C). Cultures depleted of Nrx-IV showed only 12.5±0.7% and 23.4±6.2% of Wrapper+ cells in aggregates, respectively. By contrast,in cells treated with negative control dsRNA, 93.1±0.1% of Wrapper+ cells were found in aggregates of two or more cells. These observations indicated that Wrapper and Nrx-IV were able to induce cell adhesion only when both proteins were present.

To analyze Nrx-IV and Wrapper protein localization in the aggregates, we examined the small aggregates generated by transfection of wrapperalone. wrapper-transfected cells were readily identifiable by their prominent cortical labeling with anti-Wrapper antibody(Fig. 6G-I). The sites of cell-cell contact with Wrapper- cells displayed a pronounced recruitment of Nrx-IV into cortical patches(Fig. 6G-I); these were never observed in untransfected cells. We did not observe enrichment of Wrapper to Nrx-IV+ patches, and did not see Nrx-IV+ patch formation at sites where two Wrapper+ cells were in contact(Fig. 6H,I). This phenomenon was also observed in en-Gal4 UAS-wrapper embryos, in which Nrx-IV was absent from sites of contact between Wrapper+ cells(Fig. 5F). This result might be due to the ability of high levels of Wrapper to saturably bind pools of Nrx-IV intracellularly - this competition would not leave sufficient Nrx-IV to bind to Wrapper in adjacent cells and form observable Nrx-IV+ membrane patches. Alternatively, Nrx-IV-Wrapper intracellular interactions could inhibit Nrx-IV transport and assembly into the membrane, or promote its internalization or degradation. Overall, these data show that expression of wrapper causes membrane accumulation of Nrx-IV similar to that observed in the embryonic CNS.

In this paper, we provide genetic, cellular and biochemical evidence that Nrx-IV and Wrapper physically interact to mediate cell adhesion between MG and neurons. The data presented here on Nrx-IV and wrappermutant phenotypes are in close agreement with results previously published for wrapper (Noordermeer et al.,1998), and show that both genes have identical MG phenotypes, a likely outcome for two genes involved in heterophilic cell adhesion. The Nrx-IV-Wrapper interactions occur between MG and three neuronal cell types or structures: (1) MP1s, (2) cell bodies of lateral CNS neurons, and (3)commissural axons. Together, these interactions control the position of MG and the ensheathment and subdivision of commissures.

MG-MP1 interaction

In the present paper, we provide direct evidence that MP1 neurons closely interact with AMG, suggesting an important role in their development. Beginning at late stage 12, there is a strong accumulation of Nrx-IV at the interface between the MP1s and a subset of AMG, and the Nrx-IV concentration is maintained as the AMG migrate and ensheath the commissures. Nrx-IV accumulation at the MG-MP1 boundaries was abolished in wrappermutants, and both Nrx-IV and wrapper mutants had gaps between the AMG and MP1s. These results indicate that the MP1s physically adhere to the AMG, and this adhesive interaction is required for proper positioning of MG and ensheathment of the commissures.

MG-lateral CNS neuronal cell body interaction

Nrx-IV protein is present in most, if not all, CNS neurons. However,protein levels are generally low. The exceptions are the neurons that flank the MG. These cells show a strong accumulation of Nrx-IV at the interfaces with MG. This indicates an aspect of midline cell biology not commonly considered - that MG interact closely with adjacent lateral CNS neurons. This might act to physically constrain migrating MG at the midline and restrict their lateral movement. In this sense, the lateral CNS neurons, the MP1 neurons and axon commissures work together to construct the MG cytoarchitectural scaffold. Alternatively, the adhesion between lateral CNS neurons and MG might allow developmental signals to pass between these cell types.

MG-commissural axon interaction

A key functional role of MG is their interaction with commissural axons,and these interactions require complex MG movements and morphological changes. The AMG extend cytoplasmic processes between the commissures, followed by an AMG cell body. These MG structures effectively partition the AC from the PC. Previously, it was proposed that commissure separation is caused by the interposition of MG into the unseparated commissure(Klambt et al., 1991). However, we noted that in wrapper mutants, the AC and PC were well separated, even though MG processes were commonly absent between the commissures (Fig. 4F-H). It is possible that in wrapper mutants, MG initially caused commissure separation and then quickly retracted or underwent apoptosis, indicating that MG function was required only transiently. Alternatively, commissure separation could be independent of MG interposition and the MG partition already-separated commissures. In contrast to wrapper mutants, Nrx-IV mutants have poorly separated commissures. This difference is most likely to reflect an additional function of Nrx-IV because: (1)the MG phenotypes were similar between Nrx-IV and wrappermutants, (2) the mutants of each gene were null, (3) neither had a recognizable maternal effect, and (4) Nrx-IV was more widely expressed.

Throughout commissure ensheathment, axons have strong accumulations of Nrx-IV along their interface with the AMG. This suggests a continual requirement of Nrx-IV and Wrapper to mediate MG-axonal adhesion and is consistent with the wide variety of MG-axon adhesion defects observed in both Nrx-IV and wrapper mutants and the inability of elav-Gal4 UAS-Nrx-IV to rescue late Nrx-IV mutant phenotypes. By contrast, MG remained relatively well associated with each other, suggesting that neither wrapper nor Nrx-IV plays an important role in MG-MG adhesion.

MG projections also subdivide each commissure into discrete compartments. Previous work employing electron microscopy proposed that the MG subdivided each commissure into three dorsoventral regions(Stollewerk and Klambt, 1997). This subdivision also requires Nrx-IV and wrapper function because Nrx-IV and Wrapper accumulated in the AMG commissural projections, and the projections were absent in both Nrx-IV and wrappermutants (Noordermeer et al.,1998). Both the organizing principles and the significance of these commissural subdomains are unknown, and it remains to be determined whether the MG are a cause of the subdivision or are filling in axonal regions that are already subdivided.

Perspectives on MG migration

The view of MG migration presented here builds on previous work, but also differs in several aspects. These include nomenclature, MG-neuron interactions and PMG migration. Klambt et al. (Klambt et al., 1991) proposed a model in which three pairs of MG (MGA,MGM and MGP) arise in the anterior of the segment and, during migration,separate and ensheath the AC and PC. The MGA and MGM migrate posteriorly and ensheath the AC; the MGA ultimately resides anterior to the AC and the MGM between the AC and PC. By contrast, the MGP migrate anteriorly from the adjacent posterior segment and partially ensheath the PC. More recent observations, including some from this paper, point toward a different view. Analysis of 52 genes expressed in MG(Kearney et al., 2004)indicates that (to date) only two distinct MG cell types can be identified,which we have termed AMG and PMG. There are six AMG in the anterior of the segment (this class includes MGA and MGM, which, to our knowledge, cannot be distinguished molecularly) and four PMG that reside in the posterior of the segment and are identical to MGP in terms of gene expression. Of the six initial AMG, only three survive (Bergmann et al., 2002). These cells migrate posteriorly, ensheath both the AC and PC, and elaborate projections into the commissures. By contrast, all PMG die by stage 17 (Dong and Jacobs,1997; Sonnenfeld and Jacobs,1995), and therefore do not ensheath the PC. Initially, it was proposed that PMG/MGP migrate from the adjacent posterior segment. In our experiments, we see no evidence for this. Instead, PMG arise in the En+ posterior of the segment and migrate anterodorsally toward the commissure. Before undergoing apoptosis, a single PMG abuts the PC from the posterior side. Thus, the PMG are positioned to influence commissure development.

Neurexin IV and immunoglobulin superfamily protein interactions

The experiments described in this paper strongly support the view that Nrx-IV and Wrapper directly bind and mediate cell adhesion. By contrast,neither protein mediates homophilic cell adhesion(Baumgartner et al., 1996; Noordermeer et al., 1998). Wrapper is an Ig superfamily protein, and experiments in both flies and vertebrates indicate that Nrx-IV can bind to additional Ig superfamily proteins. In Drosophila septate junctions, Nrx-IV forms a complex with Contactin and Neuroglian(Faivre-Sarrailh et al.,2004), which are two Ig superfamily proteins. It was proposed that Nrx-IV binds to Contactin at the membrane in a cis configuration(Faivre-Sarrailh et al.,2004), and that Contactin is required for proper Nrx-IV membrane localization (Laval et al.,2008). Contactin is present in the CNS, and might play a similar role in neurons. It is unknown whether Nrx-IV binds Neuroglian or Contactin in trans, similar to the mechanism proposed here for Nrx-IV-Wrapper binding. However, at paranodal axo-glial junctions in mice, the Nrx-IV homolog Caspr binds in cis to contactin, and in trans to the Neuroglian homolog neurofascin. In summary, Nrx-IV binding to Wrapper indicates a general feature of Nrx-IV,which is its ability to bind diverse Ig superfamily proteins.

One of the remarkable aspects of Nrx-IV is its strong membrane accumulation at sites where neurons are apposed to MG. In one sense, this resembles the accumulation of Nrx-IV in septate junctions. However, from a mechanistic perspective the situation appears different. In septate junctions, Nrx-IV membrane localization is constrained by interactions with Contactin and Neuroglian (Faivre-Sarrailh et al.,2004; Laval et al.,2008), as well as with cytoskeleton-associated proteins important for membrane localization (Laval et al.,2008; Ward et al.,1998b; Wu et al.,2007). By contrast, the localization of Nrx-IV in neurons appears relatively fluid and dispersed, only accumulating at high levels when in contact with a Wrapper+ membrane. It remains possible that once Wrapper and Nrx-IV bind, additional proteins might bind to Nrx-IV to stabilize its membrane localization. These interactions could further regulate the dynamics of MG-neuron interactions.

We thank Jasperina Noordermeer for fly stocks; Raehum Paik for generating the anti-Wrapper guinea pig antibody; the Developmental Studies Hybridoma Bank, the Bloomington Drosophila Stock Center, the East Asian Distribution Center for Segmentation Antibodies; and FlyTrap for strains and reagents. This work was supported by NIH grants R37 RD25251 to S.T.C., NS050356 to M.A.B., GM081645 to S.L.R. and an NRSA postdoctoral fellowship to S.R.W. Deposited in PMC for release after 12 months.

Banerjee, S. and Bhat, M. A. (
2007
). Neuron-glial interactions in blood-brain barrier formation.
Annu. Rev. Neurosci.
30
,
235
-258.
Banerjee, S., Pillai, A. M., Paik, R., Li, J. and Bhat, M. A. (
2006a
). Axonal ensheathment and septate junction formation in the peripheral nervous system of Drosophila.
J. Neurosci.
26
,
3319
-3329.
Banerjee, S., Sousa, A. D. and Bhat, M. A.(
2006b
). Organization and function of septate junctions: an evolutionary perspective.
Cell Biochem. Biophys.
46
,
65
-77.
Baumgartner, S., Littleton, J. T., Broadie, K., Bhat, M. A.,Harbecke, R., Lengyel, J. A., Chiquet-Ehrismann, R., Prokop, A. and Bellen, H. J. (
1996
). A Drosophila neurexin is required for septate junction and blood-nerve barrier formation and function.
Cell
87
,
1059
-1068.
Bergmann, A., Tugentman, M., Shilo, B. Z. and Steller, H.(
2002
). Regulation of cell number by MAPK-dependent control of apoptosis: a mechanism for trophic survival signaling.
Dev. Cell
2
,
159
-170.
Bhat, M. A. (
2003
). Molecular organization of axo-glial junctions.
Curr. Opin. Neurobiol.
13
,
552
-559.
Brand, A. (
1995
). GFP in Drosophila.
Trends Genet
.
11
,
324
-325.
Broihier, H. T. and Skeath, J. B. (
2002
). Drosophila homeodomain protein dHb9 directs neuronal fate via crossrepressive and cell-nonautonomous mechanisms.
Neuron
35
,
39
-50.
Buszczak, M., Paterno, S., Lighthouse, D., Bachman, J., Planck,J., Owen, S., Skora, A. D., Nystul, T. G., Ohlstein, B., Allen, A. et al.(
2007
). The carnegie protein trap library: a versatile tool for Drosophila developmental studies.
Genetics
175
,
1505
-1531.
Calleja, M., Moreno, E., Pelaz, S. and Morata, G.(
1996
). Visualization of gene expression in living adult Drosophila.
Science
274
,
252
-255.
Campbell, R. M. and Peterson, A. C. (
1993
). Expression of a lacZ transgene reveals floor plate cell morphology and macromolecular transfer to commissural axons.
Development
119
,
1217
-1228.
Charles, P., Tait, S., Faivre-Sarrailh, C., Barbin, G.,Gunn-Moore, F., Denisenko-Nehrbass, N., Guennoc, A. M., Girault, J. A.,Brophy, P. J. and Lubetzki, C. (
2002
). Neurofascin is a glial receptor for the paranodin/Caspr-contactin axonal complex at the axoglial junction.
Curr. Biol.
12
,
217
-220.
Dong, R. and Jacobs, J. R. (
1997
). Origin and differentiation of supernumerary midline glia in Drosophila embryos deficient for apoptosis.
Dev. Biol.
190
,
165
-177.
Faivre-Sarrailh, C., Banerjee, S., Li, J., Hortsch, M., Laval,M. and Bhat, M. A. (
2004
). Drosophila contactin, a homolog of vertebrate contactin, is required for septate junction organization and paracellular barrier function.
Development
131
,
4931
-4942.
Garbe, D. S. and Bashaw, G. J. (
2004
). Axon guidance at the midline: from mutants to mechanisms.
Crit. Rev. Biochem. Mol. Biol.
39
,
319
-341.
Han, K., Levine, M. S. and Manley, J. L.(
1989
). Synergistic activation and repression of transcription by Drosophila homeobox proteins.
Cell
56
,
573
-583.
Hortsch, M. and Bieber, A. J. (
1991
). Sticky molecules in not-so-sticky cells.
Trends Biochem. Sci.
16
,
283
-287.
Jacobs, J. R. (
2000
). The midline glia of Drosophila: a molecular genetic model for the developmental functions of glia.
Prog. Neurobiol.
62
,
475
-508.
Kearney, J. B., Wheeler, S. R., Estes, P., Parente, B. and Crews, S. T. (
2004
). Gene expression profiling of the developing Drosophila CNS midline cells.
Dev. Biol.
275
,
473
-492.
Klambt, C., Jacobs, J. R. and Goodman, C. S.(
1991
). The midline of the Drosophila central nervous system: a model for the genetic analysis of cell fate, cell migration, and growth cone guidance.
Cell
64
,
801
-815.
Kosman, D., Small, S. and Reinitz, J. (
1998
). Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins.
Dev. Genes Evol.
208
,
290
-294.
Laval, M., Bel, C. and Faivre-Sarrailh, C.(
2008
). The lateral mobility of cell adhesion molecules is highly restricted at septate junctions in Drosophila.
BMC Cell Biol.
9
,
38
-49.
Learte, A. R., Forero, M. G. and Hidalgo, A.(
2008
). Gliatrophic and gliatropic roles of PVF/PVR signaling during axon guidance.
Glia
56
,
164
-176.
Lin, D. M. and Goodman, C. S. (
1994
). Ectopic and increased expression of Fasciclin II alters motoneuron growth cone guidance.
Neuron
13
,
507
-523.
Miguel-Aliaga, I. and Thor, S. (
2004
). Segment-specific prevention of pioneer neuron apoptosis by cell-autonomous,postmitotic Hox gene activity.
Development
131
,
6093
-6105.
Morin, X., Daneman, R., Zavortink, M. and Chia, W.(
2001
). A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila.
Proc. Natl. Acad. Sci. USA
98
,
15050
-15055.
Noordermeer, J. N., Kopczynski, C. C., Fetter, R. D., Bland, K. S., Chen, W. Y. and Goodman, C. S. (
1998
). Wrapper, a novel member of the Ig superfamily, is expressed by midline glia and is required for them to ensheath commissural axons in Drosophila.
Neuron
21
,
991
-1001.
Patel, N. H., Kornberg, T. B. and Goodman, C. S.(
1989
). Expression of engrailed during segmentation in grasshopper and crayfish.
Development
107
,
201
-212.
Rogers, S. L. and Rogers, G. C. (
2008
). Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy.
Nat. Protoc.
3
,
606
-611.
Sanson, B., White, P. and Vincent, J. P.(
1996
). Uncoupling cadherin-based adhesion from wingless signalling in Drosophila.
Nature
383
,
627
-630.
Scholz, H., Sadlowski, E., Klaes, A. and Klambt, C.(
1997
). Control of midline glia development in the embryonic Drosophila CNS.
Mech. Dev.
64
,
137
-151.
Shiga, Y., Tanaka-Matakatsu, M. and Hayashi, S.(
1996
). A nuclear GFP/b-galactosidase fusion protein as a marker for morphogenesis in living Drosophila.
Dev. Growth Differ.
38
,
99
-106.
Sonnenfeld, M. J. and Jacobs, J. R. (
1995
). Apoptosis of the midline glia during Drosophila embryogenesis: a correlation with axon contact.
Development
121
,
569
-578.
Stollewerk, A. and Klambt, C. (
1997
). The midline glial cells are required for regionalization of commissural axons in the embryonic CNS of Drosophila.
Dev. Genes Evol.
207
,
402
-409.
Ward, M. P., Mosher, J. T. and Crews, S. T.(
1998a
). Regulation of bHLH-PAS protein subcellular localization during Drosophila embryogenesis.
Development
125
,
1599
-1608.
Ward, R. E., 4th, Lamb, R. S. and Fehon, R. G.(
1998b
). A conserved functional domain of Drosophila coracle is required for localization at the septate junction and has membrane-organizing activity.
J. Cell Biol.
140
,
1463
-1473.
Wheeler, S. R., Kearney, J. B., Guardiola, A. R. and Crews, S. T. (
2006
). Single-cell mapping of neural and glial gene expression in the developing Drosophila CNS midline cells.
Dev. Biol.
294
,
509
-524.
Wheeler, S. R., Stagg, S. B. and Crews, S. T.(
2008
). Multiple Notch signaling events control Drosophila CNS midline neurogenesis, gliogenesis and neuronal identity.
Development
135
,
3071
-3079.
Wu, V. M., Yu, M. H., Paik, R., Banerjee, S., Liang, Z., Paul,S. M., Bhat, M. A. and Beitel, G. J. (
2007
). Drosophila Varicose, a member of a new subgroup of basolateral MAGUKs, is required for septate junctions and tracheal morphogenesis.
Development
134
,
999
-1009.
Xiao, H., Hrdlicka, L. A. and Nambu, J. R.(
1996
). Alternate functions of the single-minded and rhomboid genes in development of the Drosophila ventral neuroectoderm.
Mech. Dev.
58
,
65
-74.
Yoshioka, T. and Tanaka, O. (
1989
). Ultrastructural and cytochemical characterisation of the floor plate ependyma of the developing rat spinal cord.
J. Anat.
165
,
87
-100.

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