Stress and survival of juvenile New Zealand green-lipped mussel, Perna canaliculus, is a poorly-understood bottleneck in the ecological and economic performance of a significant aquaculture crop. This species was therefore selected as a model organism for the development of a new method to quantify oxidative stress in whole individuals. An in vivo ROS-activated stain (CellROX™) was administered to anaesthetised, translucent juveniles that were subsequently formaldehyde-fixed and visualised using confocal microscopy. Subsequent application of image analysis to quantifying ROS-positive tissue areas was successfully used to detect stress differences in juvenile mussels exposed to varying levels of emersion. This integrated method can be used to localise and quantify ROS production in individual translucent bivalve life stages (larval and juvenile), while relative stability following fixation greatly expands potential practical field applications.
A new method to localise and quantify oxidative stress in live juvenile mussels
- Award Group:
- Funder(s): Ministry for Business, Innovation and Employment, New Zealand
- Award Id(s): CAWX1801
- Funder(s):
Currently Viewing Accepted Manuscript - Newer Version Available
- Split-screen
- Views Icon Views
- Open the PDF for in another window
-
Article Versions Icon
Versions
- Version of Record 14 December 2021
- Accepted Manuscript 29 November 2021
- Share Icon Share
-
Tools Icon
Tools
- Search Site
Natalí J. Delorme, Alfonso J. Schmidt, Leonardo N. Zamora, David J. Burritt, Norman L. C. Ragg; A new method to localise and quantify oxidative stress in live juvenile mussels. Biol Open 2021; bio.059030. doi: https://doi.org/10.1242/bio.059030
Download citation file:
Advertisement
Read & Publish Open Access publishing: what authors say
We have had great feedback from authors who have benefitted from our Read & Publish agreement with their institution and have been able to publish Open Access with us without paying an APC. Read what they had to say.
Biology Open 2023 - a year in review
In this Editorial, BiO Editor-in-Chief Dan Gorelick reviews the journal's performance in 2023 and discusses his plans to improve author experience.
The Forest of Biologists
Our Publisher Claire Moulton recently visited the two Woodland Trust UK sites where we are planting new native trees for published Research and Review papers and protecting ancient woodland on behalf of our peer reviewers.
A Year at the Forefront
This series of Review articles aims to highlight the key discoveries, technological innovations, new resources and new hypotheses that have made an impact in a specific biological field during the past year. This publishing opportunity is available to early-career researchers, without a publication charge. Find out about eligibility and how to submit a proposal.
How we support early-career researchers
Biology Open, its sister journals and its not-for-profit publisher, The Company of Biologists, support early-career researchers in numerous ways, helping them grow their network and raise their profile. Find out what we can do to support you.