The tracheal basal cells (BCs) function as stem cells to maintain the epithelium in steady state and repair it after injury. The airway is surrounded by cartilage ventrolaterally and smooth muscle dorsally. Lineage tracing using Krt5-CreER shows dorsal BCs produce more, larger, clones than ventral BCs. Large clones were found between cartilage and smooth muscle where subpopulation of dorsal BCs exists. Three-dimensional organoid culture of BCs demonstrated that dorsal BCs show higher colony forming efficacy to ventral BCs. Gene ontology analysis revealed that genes expressed in dorsal BCs are enriched in wound healing while ventral BCs are enriched in response to external stimulus and immune response. Significantly, ventral BCs express Myostatin, which inhibits the growth of smooth muscle cells, and HGF, which facilitates cartilage repair. The results support the hypothesis that BCs from the dorso-ventral airways have intrinsic molecular and behavioural differences relevant to their in vivo function.
Dorso-ventral heterogeneity in tracheal basal stem cells
- Award Group:
- Funder(s): Japan Society for the Promotion of Science
- Award Id(s): JP16K19462
- Funder(s):
- Award Group:
- Funder(s): NODAI Genome Research Center, Tokyo University of Agriculture
- Award Id(s): Cooperative Research Grant of the Genome Research for BioResource
- Funder(s):
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Tomomi Tadokoro, Keisuke Tanaka, Shun Osakabe, Mimoko Kato, Hisato Kobayashi, Brigid L.M. Hogan, Hideki Taniguchi; Dorso-ventral heterogeneity in tracheal basal stem cells. Biol Open 2021; bio.058676. doi: https://doi.org/10.1242/bio.058676
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