The scanty information on astrogenesis following stressor effect, albeit imperative roles of astroglia in normal physiology and pathophysiology, incited us to assess temporal astrogenesis and astrocyte density in an intra-generational protein malnutrition (PMN) rat model. Standard immunohistochemical procedures for glial lineage markers and their intensity measurements and qRT-PCR studies were performed to reveal the spatio-temporal origin and density of astrocytes. Reduced A2B5+ glia restricted precursor population in ventricles and poor dissemination to cortex at embryonic days (E)11-14, low BLBP+ secondary radial glia in SVZ of E16 LP brains reflect compromised progenitor pooling. Contrary to large sized BLBP+ gliospheres in HP at E16, small gliospheres and discrete BLBP+ cells in LP brains evidence loss of colonization and low proliferative potential. Delayed emergence of GFAP expression, precocious astrocyte maturation and significantly reduced astrocyte number speculate impaired temporal and compromised astrogenesis within LP-F1 brains. Our findings of protein deprivation induced impairments in temporal astrogenesis, compromised density and astrocytic dysfunction, strengthen the hypothesis “astrocytes as possible drivers of neurodevelopmental disorders” and may increase our understanding of stressor associated brain development, opening up windows for effective therapeutic interventions against debilitating neurodevelopmental disorders.
Intra-generational protein malnutrition impairs temporal astrogenesis in rat brain
Currently Viewing Accepted Manuscript - Newer Version Available
- Split-screen
- Views Icon Views
- Open the PDF for in another window
-
Article Versions Icon
Versions
- Version of Record 15 July 2017
- Accepted Manuscript 01 January 2017
- Share Icon Share
-
Tools Icon
Tools
- Search Site
Aijaz Ahmad Naik, Nisha Patro, Pankaj Seth, Ishan K. Patro; Intra-generational protein malnutrition impairs temporal astrogenesis in rat brain. Biol Open 2017; bio.023432. doi: https://doi.org/10.1242/bio.023432
Download citation file:
Advertisement
Read & Publish Open Access publishing: what authors say
We have had great feedback from authors who have benefitted from our Read & Publish agreement with their institution and have been able to publish Open Access with us without paying an APC. Read what they had to say.
Gatekeeping at BiO
In his Editorial, BiO Editor-in-Chief Dan Gorelick outlines the criteria by which articles submitted to BiO are assessed, as part of initiatives to increase transparency of journal 'gatekeeping'.
The Forest of Biologists
Our Publisher Claire Moulton recently visited the two Woodland Trust UK sites where we are planting new native trees for published Research and Review papers and protecting ancient woodland on behalf of our peer reviewers.
A Year at the Forefront
This series of Review articles aims to highlight the key discoveries, technological innovations, new resources and new hypotheses that have made an impact in a specific biological field during the past year. This publishing opportunity is available to early-career researchers, without a publication charge. Find out about eligibility and how to submit a proposal.
How we support early-career researchers
Biology Open, its sister journals and its not-for-profit publisher, The Company of Biologists, support early-career researchers in numerous ways, helping them grow their network and raise their profile. Find out what we can do to support you.